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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-13087937-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=13087937&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 13087937,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001367363.2",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.604G>A",
          "hgvs_p": "p.Ala202Thr",
          "transcript": "NM_001080555.4",
          "protein_id": "NP_001074024.1",
          "transcript_support_level": null,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 604,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000651961.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001080555.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.604G>A",
          "hgvs_p": "p.Ala202Thr",
          "transcript": "ENST00000651961.1",
          "protein_id": "ENSP00000498528.1",
          "transcript_support_level": null,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 604,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001080555.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000651961.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.535G>A",
          "hgvs_p": "p.Ala179Thr",
          "transcript": "ENST00000432710.7",
          "protein_id": "ENSP00000405032.2",
          "transcript_support_level": 1,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 339,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 1020,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000432710.7"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.496G>A",
          "hgvs_p": "p.Ala166Thr",
          "transcript": "ENST00000337630.10",
          "protein_id": "ENSP00000336816.6",
          "transcript_support_level": 1,
          "aa_start": 166,
          "aa_end": null,
          "aa_length": 326,
          "cds_start": 496,
          "cds_end": null,
          "cds_length": 981,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000337630.10"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.728G>A",
          "hgvs_p": "p.Arg243His",
          "transcript": "ENST00000351606.10",
          "protein_id": "ENSP00000336857.6",
          "transcript_support_level": 1,
          "aa_start": 243,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 728,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000351606.10"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.620G>A",
          "hgvs_p": "p.Arg207His",
          "transcript": "ENST00000396302.7",
          "protein_id": "ENSP00000379596.3",
          "transcript_support_level": 1,
          "aa_start": 207,
          "aa_end": null,
          "aa_length": 285,
          "cds_start": 620,
          "cds_end": null,
          "cds_length": 858,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000396302.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "FAM234B",
          "gene_hgnc_id": 29288,
          "hgvs_c": "c.970+11794C>T",
          "hgvs_p": null,
          "transcript": "ENST00000537625.1",
          "protein_id": "ENSP00000437974.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 324,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 975,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000537625.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.443-674G>A",
          "hgvs_p": null,
          "transcript": "ENST00000537302.5",
          "protein_id": "ENSP00000441718.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 298,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 897,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000537302.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.724G>A",
          "hgvs_p": "p.Ala242Thr",
          "transcript": "NM_001367363.2",
          "protein_id": "NP_001354292.1",
          "transcript_support_level": null,
          "aa_start": 242,
          "aa_end": null,
          "aa_length": 402,
          "cds_start": 724,
          "cds_end": null,
          "cds_length": 1209,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001367363.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.676G>A",
          "hgvs_p": "p.Ala226Thr",
          "transcript": "NM_001367359.2",
          "protein_id": "NP_001354288.1",
          "transcript_support_level": null,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 386,
          "cds_start": 676,
          "cds_end": null,
          "cds_length": 1161,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001367359.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.604G>A",
          "hgvs_p": "p.Ala202Thr",
          "transcript": "NM_001367362.2",
          "protein_id": "NP_001354291.1",
          "transcript_support_level": null,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 371,
          "cds_start": 604,
          "cds_end": null,
          "cds_length": 1116,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001367362.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.728G>A",
          "hgvs_p": "p.Arg243His",
          "transcript": "NM_001367358.2",
          "protein_id": "NP_001354287.1",
          "transcript_support_level": null,
          "aa_start": 243,
          "aa_end": null,
          "aa_length": 354,
          "cds_start": 728,
          "cds_end": null,
          "cds_length": 1065,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001367358.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.565G>A",
          "hgvs_p": "p.Ala189Thr",
          "transcript": "NM_001368007.2",
          "protein_id": "NP_001354936.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 349,
          "cds_start": 565,
          "cds_end": null,
          "cds_length": 1050,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001368007.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.800G>A",
          "hgvs_p": "p.Arg267His",
          "transcript": "NM_001367361.2",
          "protein_id": "NP_001354290.1",
          "transcript_support_level": null,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001367361.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.535G>A",
          "hgvs_p": "p.Ala179Thr",
          "transcript": "NM_001206842.3",
          "protein_id": "NP_001193771.1",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 339,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 1020,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001206842.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.496G>A",
          "hgvs_p": "p.Ala166Thr",
          "transcript": "NM_153823.5",
          "protein_id": "NP_722545.2",
          "transcript_support_level": null,
          "aa_start": 166,
          "aa_end": null,
          "aa_length": 326,
          "cds_start": 496,
          "cds_end": null,
          "cds_length": 981,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_153823.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.728G>A",
          "hgvs_p": "p.Arg243His",
          "transcript": "NM_001080554.4",
          "protein_id": "NP_001074023.1",
          "transcript_support_level": null,
          "aa_start": 243,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 728,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001080554.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.659G>A",
          "hgvs_p": "p.Arg220His",
          "transcript": "NM_001367364.2",
          "protein_id": "NP_001354293.1",
          "transcript_support_level": null,
          "aa_start": 220,
          "aa_end": null,
          "aa_length": 298,
          "cds_start": 659,
          "cds_end": null,
          "cds_length": 897,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001367364.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.659G>A",
          "hgvs_p": "p.Arg220His",
          "transcript": "ENST00000545401.6",
          "protein_id": "ENSP00000445884.2",
          "transcript_support_level": 3,
          "aa_start": 220,
          "aa_end": null,
          "aa_length": 298,
          "cds_start": 659,
          "cds_end": null,
          "cds_length": 897,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000545401.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GSG1",
          "gene_hgnc_id": 19716,
          "hgvs_c": "c.620G>A",
          "hgvs_p": "p.Arg207His",
          "transcript": "NM_031289.5",
          "protein_id": "NP_112579.2",
          "transcript_support_level": null,
          "aa_start": 207,
          "aa_end": null,
          "aa_length": 285,
          "cds_start": 620,
          "cds_end": null,
          "cds_length": 858,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_031289.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
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        {
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          "gene_symbol": "GSG1",
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          "feature": "ENST00000542415.5"
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      ],
      "gene_symbol": "GSG1",
      "gene_hgnc_id": 19716,
      "dbsnp": "rs768853667",
      "frequency_reference_population": 0.00001796758,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 29,
      "gnomad_exomes_af": 0.0000177861,
      "gnomad_genomes_af": 0.0000197109,
      "gnomad_exomes_ac": 26,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.24770376086235046,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.384,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1469,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.28,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.492,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001367363.2",
          "gene_symbol": "GSG1",
          "hgnc_id": 19716,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.724G>A",
          "hgvs_p": "p.Ala242Thr"
        },
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000537625.1",
          "gene_symbol": "FAM234B",
          "hgnc_id": 29288,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.970+11794C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}