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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-44523364-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=44523364&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 44523364,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001145107.2",
      "consequences": [
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NELL2",
          "gene_hgnc_id": 7751,
          "hgvs_c": "c.1925A>G",
          "hgvs_p": "p.His642Arg",
          "transcript": "NM_001145108.2",
          "protein_id": "NP_001138580.1",
          "transcript_support_level": null,
          "aa_start": 642,
          "aa_end": null,
          "aa_length": 816,
          "cds_start": 1925,
          "cds_end": null,
          "cds_length": 2451,
          "cdna_start": 2371,
          "cdna_end": null,
          "cdna_length": 3553,
          "mane_select": "ENST00000429094.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145108.2"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NELL2",
          "gene_hgnc_id": 7751,
          "hgvs_c": "c.1925A>G",
          "hgvs_p": "p.His642Arg",
          "transcript": "ENST00000429094.7",
          "protein_id": "ENSP00000390680.2",
          "transcript_support_level": 1,
          "aa_start": 642,
          "aa_end": null,
          "aa_length": 816,
          "cds_start": 1925,
          "cds_end": null,
          "cds_length": 2451,
          "cdna_start": 2371,
          "cdna_end": null,
          "cdna_length": 3553,
          "mane_select": "NM_001145108.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000429094.7"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NELL2",
          "gene_hgnc_id": 7751,
          "hgvs_c": "c.1925A>G",
          "hgvs_p": "p.His642Arg",
          "transcript": "ENST00000452445.6",
          "protein_id": "ENSP00000394612.2",
          "transcript_support_level": 1,
          "aa_start": 642,
          "aa_end": null,
          "aa_length": 816,
          "cds_start": 1925,
          "cds_end": null,
          "cds_length": 2451,
          "cdna_start": 2018,
          "cdna_end": null,
          "cdna_length": 3196,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000452445.6"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NELL2",
          "gene_hgnc_id": 7751,
          "hgvs_c": "c.1922A>G",
          "hgvs_p": "p.His641Arg",
          "transcript": "ENST00000395487.6",
          "protein_id": "ENSP00000378866.2",
          "transcript_support_level": 1,
          "aa_start": 641,
          "aa_end": null,
          "aa_length": 815,
          "cds_start": 1922,
          "cds_end": null,
          "cds_length": 2448,
          "cdna_start": 2018,
          "cdna_end": null,
          "cdna_length": 3203,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395487.6"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NELL2",
          "gene_hgnc_id": 7751,
          "hgvs_c": "c.1781A>G",
          "hgvs_p": "p.His594Arg",
          "transcript": "ENST00000551601.5",
          "protein_id": "ENSP00000449332.1",
          "transcript_support_level": 1,
          "aa_start": 594,
          "aa_end": null,
          "aa_length": 768,
          "cds_start": 1781,
          "cds_end": null,
          "cds_length": 2307,
          "cdna_start": 1970,
          "cdna_end": null,
          "cdna_length": 3148,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000551601.5"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NELL2",
          "gene_hgnc_id": 7751,
          "hgvs_c": "c.2075A>G",
          "hgvs_p": "p.His692Arg",
          "transcript": "NM_001145107.2",
          "protein_id": "NP_001138579.1",
          "transcript_support_level": null,
          "aa_start": 692,
          "aa_end": null,
          "aa_length": 866,
          "cds_start": 2075,
          "cds_end": null,
          "cds_length": 2601,
          "cdna_start": 2225,
          "cdna_end": null,
          "cdna_length": 3407,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145107.2"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NELL2",
          "gene_hgnc_id": 7751,
          "hgvs_c": "c.2075A>G",
          "hgvs_p": "p.His692Arg",
          "transcript": "ENST00000437801.6",
          "protein_id": "ENSP00000416341.2",
          "transcript_support_level": 2,
          "aa_start": 692,
          "aa_end": null,
          "aa_length": 866,
          "cds_start": 2075,
          "cds_end": null,
          "cds_length": 2601,
          "cdna_start": 2447,
          "cdna_end": null,
          "cdna_length": 3279,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000437801.6"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NELL2",
          "gene_hgnc_id": 7751,
          "hgvs_c": "c.1994A>G",
          "hgvs_p": "p.His665Arg",
          "transcript": "NM_001145110.2",
          "protein_id": "NP_001138582.1",
          "transcript_support_level": null,
          "aa_start": 665,
          "aa_end": null,
          "aa_length": 839,
          "cds_start": 1994,
          "cds_end": null,
          "cds_length": 2520,
          "cdna_start": 2147,
          "cdna_end": null,
          "cdna_length": 3329,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145110.2"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
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          "gene_symbol": "NELL2",
          "gene_hgnc_id": 7751,
          "hgvs_c": "c.1994A>G",
          "hgvs_p": "p.His665Arg",
          "transcript": "ENST00000333837.8",
          "protein_id": "ENSP00000327988.4",
          "transcript_support_level": 2,
          "aa_start": 665,
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          "cds_start": 1994,
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          "cds_length": 2520,
          "cdna_start": 2086,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000333837.8"
        },
        {
          "aa_ref": "H",
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
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          "gene_symbol": "NELL2",
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          "hgvs_c": "c.1925A>G",
          "hgvs_p": "p.His642Arg",
          "transcript": "NM_006159.2",
          "protein_id": "NP_006150.1",
          "transcript_support_level": null,
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        {
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          ],
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          "exon_count": 21,
          "intron_rank": null,
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          "gene_symbol": "NELL2",
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          "hgvs_c": "c.1925A>G",
          "hgvs_p": "p.His642Arg",
          "transcript": "ENST00000868481.1",
          "protein_id": "ENSP00000538540.1",
          "transcript_support_level": null,
          "aa_start": 642,
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          "cds_start": 1925,
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        {
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          "hgvs_p": "p.His642Arg",
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        {
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          ],
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          "gene_symbol": "NELL2",
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          "hgvs_c": "c.1922A>G",
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        {
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          ],
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          "gene_symbol": "NELL2",
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        {
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        {
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          "gene_symbol": "NELL2",
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        {
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          "gene_symbol": "NELL2",
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        },
        {
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          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
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          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NELL2",
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          "hgvs_c": "n.272A>G",
          "hgvs_p": null,
          "transcript": "ENST00000547636.1",
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000547636.1"
        }
      ],
      "gene_symbol": "NELL2",
      "gene_hgnc_id": 7751,
      "dbsnp": "rs773598023",
      "frequency_reference_population": 6.8405257e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84053e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.85505610704422,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.835,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.1537,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.36,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 8.01,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001145107.2",
          "gene_symbol": "NELL2",
          "hgnc_id": 7751,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2075A>G",
          "hgvs_p": "p.His692Arg"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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