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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-51048366-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=51048366&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 51048366,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001243689.2",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "NM_015416.5",
          "protein_id": "NP_056231.3",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 360,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1083,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000262055.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015416.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "ENST00000262055.9",
          "protein_id": "ENSP00000262055.4",
          "transcript_support_level": 1,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 360,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1083,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_015416.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000262055.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.-52T>C",
          "hgvs_p": null,
          "transcript": "ENST00000550929.5",
          "protein_id": "ENSP00000450163.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 304,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 915,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000550929.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "n.10T>C",
          "hgvs_p": null,
          "transcript": "ENST00000547318.5",
          "protein_id": "ENSP00000448442.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000547318.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "ENST00000882989.1",
          "protein_id": "ENSP00000553048.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 405,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1218,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882989.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "ENST00000950003.1",
          "protein_id": "ENSP00000620062.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 404,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1215,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950003.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "ENST00000882986.1",
          "protein_id": "ENSP00000553045.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882986.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "NM_001243689.2",
          "protein_id": "NP_001230618.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 373,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1122,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001243689.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "ENST00000418425.6",
          "protein_id": "ENSP00000389903.2",
          "transcript_support_level": 2,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 373,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1122,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000418425.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "NM_001351315.2",
          "protein_id": "NP_001338244.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 367,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1104,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001351315.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "ENST00000882983.1",
          "protein_id": "ENSP00000553042.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 359,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1080,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882983.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "ENST00000950000.1",
          "protein_id": "ENSP00000620060.1",
          "transcript_support_level": null,
          "aa_start": 4,
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          "aa_length": 359,
          "cds_start": 10,
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          "cds_length": 1080,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950000.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "ENST00000882984.1",
          "protein_id": "ENSP00000553043.1",
          "transcript_support_level": null,
          "aa_start": 4,
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          "cds_start": 10,
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          "cds_length": 1077,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882984.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "ENST00000950004.1",
          "protein_id": "ENSP00000620063.1",
          "transcript_support_level": null,
          "aa_start": 4,
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          "aa_length": 357,
          "cds_start": 10,
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          "cdna_start": null,
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        },
        {
          "aa_ref": "S",
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          "strand": true,
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          ],
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          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "ENST00000950005.1",
          "protein_id": "ENSP00000620064.1",
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          "cds_start": 10,
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          "cds_length": 1053,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000950005.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "NM_001351316.2",
          "protein_id": "NP_001338245.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
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          "cds_start": 10,
          "cds_end": null,
          "cds_length": 1011,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "NM_001351316.2"
        },
        {
          "aa_ref": "S",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
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          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "NM_001351317.2",
          "protein_id": "NP_001338246.1",
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          "biotype": "protein_coding",
          "feature": "NM_001351317.2"
        },
        {
          "aa_ref": "S",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
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          ],
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          "intron_rank": null,
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          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "ENST00000926726.1",
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000926726.1"
        },
        {
          "aa_ref": "S",
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          "protein_coding": true,
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          "intron_rank": null,
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          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
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          "protein_id": "ENSP00000620061.1",
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          "cds_start": 10,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950002.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro",
          "transcript": "ENST00000882985.1",
          "protein_id": "ENSP00000553044.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 308,
          "cds_start": 10,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882985.1"
        },
        {
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          "intron_rank_end": null,
          "gene_symbol": "LETMD1",
          "gene_hgnc_id": 24241,
          "hgvs_c": "n.79+68T>C",
          "hgvs_p": null,
          "transcript": "NR_147098.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_147098.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000307060",
          "gene_hgnc_id": null,
          "hgvs_c": "n.-142A>G",
          "hgvs_p": null,
          "transcript": "ENST00000823174.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000823174.1"
        }
      ],
      "gene_symbol": "LETMD1",
      "gene_hgnc_id": 24241,
      "dbsnp": "rs141650005",
      "frequency_reference_population": 0.0000018587522,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000136812,
      "gnomad_genomes_af": 0.0000065735,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.33037444949150085,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.218,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1687,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 3.455,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001243689.2",
          "gene_symbol": "LETMD1",
          "hgnc_id": 24241,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.10T>C",
          "hgvs_p": "p.Ser4Pro"
        },
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000823174.1",
          "gene_symbol": "ENSG00000307060",
          "hgnc_id": null,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.-142A>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}