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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-54401586-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=54401586&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 54401586,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_002205.5",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "c.2386G>A",
          "hgvs_p": "p.Gly796Ser",
          "transcript": "NM_002205.5",
          "protein_id": "NP_002196.4",
          "transcript_support_level": null,
          "aa_start": 796,
          "aa_end": null,
          "aa_length": 1049,
          "cds_start": 2386,
          "cds_end": null,
          "cds_length": 3150,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000293379.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002205.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "c.2386G>A",
          "hgvs_p": "p.Gly796Ser",
          "transcript": "ENST00000293379.9",
          "protein_id": "ENSP00000293379.4",
          "transcript_support_level": 1,
          "aa_start": 796,
          "aa_end": null,
          "aa_length": 1049,
          "cds_start": 2386,
          "cds_end": null,
          "cds_length": 3150,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002205.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000293379.9"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "c.2440G>A",
          "hgvs_p": "p.Gly814Ser",
          "transcript": "ENST00000945317.1",
          "protein_id": "ENSP00000615376.1",
          "transcript_support_level": null,
          "aa_start": 814,
          "aa_end": null,
          "aa_length": 1067,
          "cds_start": 2440,
          "cds_end": null,
          "cds_length": 3204,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945317.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "c.2413G>A",
          "hgvs_p": "p.Gly805Ser",
          "transcript": "ENST00000945318.1",
          "protein_id": "ENSP00000615377.1",
          "transcript_support_level": null,
          "aa_start": 805,
          "aa_end": null,
          "aa_length": 1058,
          "cds_start": 2413,
          "cds_end": null,
          "cds_length": 3177,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945318.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "c.2410G>A",
          "hgvs_p": "p.Gly804Ser",
          "transcript": "ENST00000945319.1",
          "protein_id": "ENSP00000615378.1",
          "transcript_support_level": null,
          "aa_start": 804,
          "aa_end": null,
          "aa_length": 1057,
          "cds_start": 2410,
          "cds_end": null,
          "cds_length": 3174,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945319.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "c.2410G>A",
          "hgvs_p": "p.Gly804Ser",
          "transcript": "ENST00000945322.1",
          "protein_id": "ENSP00000615381.1",
          "transcript_support_level": null,
          "aa_start": 804,
          "aa_end": null,
          "aa_length": 1057,
          "cds_start": 2410,
          "cds_end": null,
          "cds_length": 3174,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945322.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "c.2405G>A",
          "hgvs_p": "p.Arg802Gln",
          "transcript": "ENST00000945320.1",
          "protein_id": "ENSP00000615379.1",
          "transcript_support_level": null,
          "aa_start": 802,
          "aa_end": null,
          "aa_length": 1052,
          "cds_start": 2405,
          "cds_end": null,
          "cds_length": 3159,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945320.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "c.2380G>A",
          "hgvs_p": "p.Gly794Ser",
          "transcript": "ENST00000945321.1",
          "protein_id": "ENSP00000615380.1",
          "transcript_support_level": null,
          "aa_start": 794,
          "aa_end": null,
          "aa_length": 1047,
          "cds_start": 2380,
          "cds_end": null,
          "cds_length": 3144,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945321.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "c.2386G>A",
          "hgvs_p": "p.Gly796Ser",
          "transcript": "ENST00000854883.1",
          "protein_id": "ENSP00000524942.1",
          "transcript_support_level": null,
          "aa_start": 796,
          "aa_end": null,
          "aa_length": 1021,
          "cds_start": 2386,
          "cds_end": null,
          "cds_length": 3066,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854883.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "c.2182G>A",
          "hgvs_p": "p.Gly728Ser",
          "transcript": "ENST00000854881.1",
          "protein_id": "ENSP00000524940.1",
          "transcript_support_level": null,
          "aa_start": 728,
          "aa_end": null,
          "aa_length": 981,
          "cds_start": 2182,
          "cds_end": null,
          "cds_length": 2946,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854881.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "c.874G>A",
          "hgvs_p": "p.Gly292Ser",
          "transcript": "XM_024448970.2",
          "protein_id": "XP_024304738.1",
          "transcript_support_level": null,
          "aa_start": 292,
          "aa_end": null,
          "aa_length": 545,
          "cds_start": 874,
          "cds_end": null,
          "cds_length": 1638,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024448970.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": 22,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "c.2307-108G>A",
          "hgvs_p": null,
          "transcript": "ENST00000854882.1",
          "protein_id": "ENSP00000524941.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1022,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3069,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854882.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "n.2197G>A",
          "hgvs_p": null,
          "transcript": "ENST00000552564.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000552564.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGA5",
          "gene_hgnc_id": 6141,
          "hgvs_c": "n.542G>A",
          "hgvs_p": null,
          "transcript": "ENST00000552583.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000552583.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "GPR84-AS1",
          "gene_hgnc_id": 56187,
          "hgvs_c": "n.48-26271C>T",
          "hgvs_p": null,
          "transcript": "ENST00000550474.5",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000550474.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "GPR84-AS1",
          "gene_hgnc_id": 56187,
          "hgvs_c": "n.106-5811C>T",
          "hgvs_p": null,
          "transcript": "ENST00000552785.1",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000552785.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "GPR84-AS1",
          "gene_hgnc_id": 56187,
          "hgvs_c": "n.167+10042C>T",
          "hgvs_p": null,
          "transcript": "ENST00000688443.3",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000688443.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "GPR84-AS1",
          "gene_hgnc_id": 56187,
          "hgvs_c": "n.214-26271C>T",
          "hgvs_p": null,
          "transcript": "ENST00000795924.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000795924.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "GPR84-AS1",
          "gene_hgnc_id": 56187,
          "hgvs_c": "n.176-26271C>T",
          "hgvs_p": null,
          "transcript": "ENST00000795925.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000795925.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "GPR84-AS1",
          "gene_hgnc_id": 56187,
          "hgvs_c": "n.461-26271C>T",
          "hgvs_p": null,
          "transcript": "ENST00000795926.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
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      "gnomad_exomes_ac": 61,
      "gnomad_genomes_ac": 5,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8426231145858765,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.421999990940094,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.654,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.4055,
      "alphamissense_prediction": "Uncertain_significance",
      "bayesdelnoaf_score": 0.09,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 4.598,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.126407148771903,
      "dbscsnv_ada_prediction": "Benign",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PP3_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PP3_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_002205.5",
          "gene_symbol": "ITGA5",
          "hgnc_id": 6141,
          "effects": [
            "missense_variant",
            "splice_region_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.2386G>A",
          "hgvs_p": "p.Gly796Ser"
        },
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000550474.5",
          "gene_symbol": "GPR84-AS1",
          "hgnc_id": 56187,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.48-26271C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}