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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-56639251-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=56639251&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 56639251,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001686.4",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1344G>T",
          "hgvs_p": "p.Glu448Asp",
          "transcript": "NM_001686.4",
          "protein_id": "NP_001677.2",
          "transcript_support_level": null,
          "aa_start": 448,
          "aa_end": null,
          "aa_length": 529,
          "cds_start": 1344,
          "cds_end": null,
          "cds_length": 1590,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000262030.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001686.4"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1344G>T",
          "hgvs_p": "p.Glu448Asp",
          "transcript": "ENST00000262030.8",
          "protein_id": "ENSP00000262030.3",
          "transcript_support_level": 1,
          "aa_start": 448,
          "aa_end": null,
          "aa_length": 529,
          "cds_start": 1344,
          "cds_end": null,
          "cds_length": 1590,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001686.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000262030.8"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1392G>T",
          "hgvs_p": "p.Glu464Asp",
          "transcript": "ENST00000904666.1",
          "protein_id": "ENSP00000574725.1",
          "transcript_support_level": null,
          "aa_start": 464,
          "aa_end": null,
          "aa_length": 545,
          "cds_start": 1392,
          "cds_end": null,
          "cds_length": 1638,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000904666.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1371G>T",
          "hgvs_p": "p.Glu457Asp",
          "transcript": "ENST00000904670.1",
          "protein_id": "ENSP00000574729.1",
          "transcript_support_level": null,
          "aa_start": 457,
          "aa_end": null,
          "aa_length": 538,
          "cds_start": 1371,
          "cds_end": null,
          "cds_length": 1617,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000904670.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1371G>T",
          "hgvs_p": "p.Glu457Asp",
          "transcript": "ENST00000904673.1",
          "protein_id": "ENSP00000574732.1",
          "transcript_support_level": null,
          "aa_start": 457,
          "aa_end": null,
          "aa_length": 538,
          "cds_start": 1371,
          "cds_end": null,
          "cds_length": 1617,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000904673.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1344G>T",
          "hgvs_p": "p.Glu448Asp",
          "transcript": "ENST00000904672.1",
          "protein_id": "ENSP00000574731.1",
          "transcript_support_level": null,
          "aa_start": 448,
          "aa_end": null,
          "aa_length": 533,
          "cds_start": 1344,
          "cds_end": null,
          "cds_length": 1602,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000904672.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1338G>T",
          "hgvs_p": "p.Glu446Asp",
          "transcript": "ENST00000918318.1",
          "protein_id": "ENSP00000588377.1",
          "transcript_support_level": null,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 527,
          "cds_start": 1338,
          "cds_end": null,
          "cds_length": 1584,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918318.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1332G>T",
          "hgvs_p": "p.Glu444Asp",
          "transcript": "ENST00000918320.1",
          "protein_id": "ENSP00000588379.1",
          "transcript_support_level": null,
          "aa_start": 444,
          "aa_end": null,
          "aa_length": 525,
          "cds_start": 1332,
          "cds_end": null,
          "cds_length": 1578,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918320.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1326G>T",
          "hgvs_p": "p.Glu442Asp",
          "transcript": "ENST00000944727.1",
          "protein_id": "ENSP00000614786.1",
          "transcript_support_level": null,
          "aa_start": 442,
          "aa_end": null,
          "aa_length": 523,
          "cds_start": 1326,
          "cds_end": null,
          "cds_length": 1572,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000944727.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1305G>T",
          "hgvs_p": "p.Glu435Asp",
          "transcript": "ENST00000918326.1",
          "protein_id": "ENSP00000588385.1",
          "transcript_support_level": null,
          "aa_start": 435,
          "aa_end": null,
          "aa_length": 516,
          "cds_start": 1305,
          "cds_end": null,
          "cds_length": 1551,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918326.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1302G>T",
          "hgvs_p": "p.Glu434Asp",
          "transcript": "ENST00000904669.1",
          "protein_id": "ENSP00000574728.1",
          "transcript_support_level": null,
          "aa_start": 434,
          "aa_end": null,
          "aa_length": 515,
          "cds_start": 1302,
          "cds_end": null,
          "cds_length": 1548,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000904669.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1212G>T",
          "hgvs_p": "p.Glu404Asp",
          "transcript": "ENST00000904674.1",
          "protein_id": "ENSP00000574733.1",
          "transcript_support_level": null,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 485,
          "cds_start": 1212,
          "cds_end": null,
          "cds_length": 1458,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000904674.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1203G>T",
          "hgvs_p": "p.Glu401Asp",
          "transcript": "ENST00000918328.1",
          "protein_id": "ENSP00000588387.1",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 482,
          "cds_start": 1203,
          "cds_end": null,
          "cds_length": 1449,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918328.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1185G>T",
          "hgvs_p": "p.Glu395Asp",
          "transcript": "ENST00000904668.1",
          "protein_id": "ENSP00000574727.1",
          "transcript_support_level": null,
          "aa_start": 395,
          "aa_end": null,
          "aa_length": 476,
          "cds_start": 1185,
          "cds_end": null,
          "cds_length": 1431,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000904668.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
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          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1131G>T",
          "hgvs_p": "p.Glu377Asp",
          "transcript": "ENST00000904667.1",
          "protein_id": "ENSP00000574726.1",
          "transcript_support_level": null,
          "aa_start": 377,
          "aa_end": null,
          "aa_length": 458,
          "cds_start": 1131,
          "cds_end": null,
          "cds_length": 1377,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000904667.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1062G>T",
          "hgvs_p": "p.Glu354Asp",
          "transcript": "ENST00000918323.1",
          "protein_id": "ENSP00000588382.1",
          "transcript_support_level": null,
          "aa_start": 354,
          "aa_end": null,
          "aa_length": 435,
          "cds_start": 1062,
          "cds_end": null,
          "cds_length": 1308,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918323.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.1002G>T",
          "hgvs_p": "p.Glu334Asp",
          "transcript": "ENST00000918319.1",
          "protein_id": "ENSP00000588378.1",
          "transcript_support_level": null,
          "aa_start": 334,
          "aa_end": null,
          "aa_length": 415,
          "cds_start": 1002,
          "cds_end": null,
          "cds_length": 1248,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918319.1"
        },
        {
          "aa_ref": "E",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.864G>T",
          "hgvs_p": "p.Glu288Asp",
          "transcript": "ENST00000918322.1",
          "protein_id": "ENSP00000588381.1",
          "transcript_support_level": null,
          "aa_start": 288,
          "aa_end": null,
          "aa_length": 369,
          "cds_start": 864,
          "cds_end": null,
          "cds_length": 1110,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918322.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.849G>T",
          "hgvs_p": "p.Glu283Asp",
          "transcript": "ENST00000918321.1",
          "protein_id": "ENSP00000588380.1",
          "transcript_support_level": null,
          "aa_start": 283,
          "aa_end": null,
          "aa_length": 364,
          "cds_start": 849,
          "cds_end": null,
          "cds_length": 1095,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918321.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP5F1B",
          "gene_hgnc_id": 830,
          "hgvs_c": "c.687G>T",
          "hgvs_p": "p.Glu229Asp",
          "transcript": "ENST00000918324.1",
          "protein_id": "ENSP00000588383.1",
          "transcript_support_level": null,
          "aa_start": 229,
          "aa_end": null,
          "aa_length": 310,
          "cds_start": 687,
          "cds_end": null,
          "cds_length": 933,
          "cdna_start": null,
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        {
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          "gene_symbol": "ATP5F1B",
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          "transcript": "ENST00000551182.1",
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        {
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          ],
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          "gene_symbol": "ATP5F1B",
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          "hgvs_c": "n.110-55G>T",
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          "transcript": "ENST00000548474.2",
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          "aa_length": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "pseudogene",
          "feature": "ENST00000548474.2"
        }
      ],
      "gene_symbol": "ATP5F1B",
      "gene_hgnc_id": 830,
      "dbsnp": "rs888471798",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": 0.000210701,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": 32,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5846431851387024,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.442,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.615,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.07,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 2.01,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001686.4",
          "gene_symbol": "ATP5F1B",
          "hgnc_id": 830,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1344G>T",
          "hgvs_p": "p.Glu448Asp"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}