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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-57230918-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=57230918&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 57230918,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_005412.6",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu",
          "transcript": "NM_005412.6",
          "protein_id": "NP_005403.2",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 504,
          "cds_start": 149,
          "cds_end": null,
          "cds_length": 1515,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000328923.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005412.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu",
          "transcript": "ENST00000328923.8",
          "protein_id": "ENSP00000333667.3",
          "transcript_support_level": 1,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 504,
          "cds_start": 149,
          "cds_end": null,
          "cds_length": 1515,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_005412.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000328923.8"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu",
          "transcript": "ENST00000557487.5",
          "protein_id": "ENSP00000452315.1",
          "transcript_support_level": 1,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 494,
          "cds_start": 149,
          "cds_end": null,
          "cds_length": 1485,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000557487.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.86C>T",
          "hgvs_p": "p.Ser29Leu",
          "transcript": "ENST00000414700.7",
          "protein_id": "ENSP00000406881.3",
          "transcript_support_level": 1,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 483,
          "cds_start": 86,
          "cds_end": null,
          "cds_length": 1452,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000414700.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "n.86C>T",
          "hgvs_p": null,
          "transcript": "ENST00000555774.5",
          "protein_id": "ENSP00000451872.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000555774.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "n.149C>T",
          "hgvs_p": null,
          "transcript": "ENST00000556825.5",
          "protein_id": "ENSP00000451169.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000556825.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "n.149C>T",
          "hgvs_p": null,
          "transcript": "ENST00000557433.5",
          "protein_id": "ENSP00000450610.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000557433.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu",
          "transcript": "ENST00000894753.1",
          "protein_id": "ENSP00000564812.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 545,
          "cds_start": 149,
          "cds_end": null,
          "cds_length": 1638,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000894753.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu",
          "transcript": "ENST00000894754.1",
          "protein_id": "ENSP00000564813.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 513,
          "cds_start": 149,
          "cds_end": null,
          "cds_length": 1542,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000894754.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu",
          "transcript": "ENST00000894751.1",
          "protein_id": "ENSP00000564810.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 509,
          "cds_start": 149,
          "cds_end": null,
          "cds_length": 1530,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000894751.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu",
          "transcript": "ENST00000925589.1",
          "protein_id": "ENSP00000595648.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 504,
          "cds_start": 149,
          "cds_end": null,
          "cds_length": 1515,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925589.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu",
          "transcript": "ENST00000925590.1",
          "protein_id": "ENSP00000595649.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 504,
          "cds_start": 149,
          "cds_end": null,
          "cds_length": 1515,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925590.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu",
          "transcript": "ENST00000925591.1",
          "protein_id": "ENSP00000595650.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 504,
          "cds_start": 149,
          "cds_end": null,
          "cds_length": 1515,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925591.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu",
          "transcript": "ENST00000925592.1",
          "protein_id": "ENSP00000595651.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 496,
          "cds_start": 149,
          "cds_end": null,
          "cds_length": 1491,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925592.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu",
          "transcript": "NM_001166356.2",
          "protein_id": "NP_001159828.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 494,
          "cds_start": 149,
          "cds_end": null,
          "cds_length": 1485,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001166356.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu",
          "transcript": "ENST00000894752.1",
          "protein_id": "ENSP00000564811.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 492,
          "cds_start": 149,
          "cds_end": null,
          "cds_length": 1479,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000894752.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.86C>T",
          "hgvs_p": "p.Ser29Leu",
          "transcript": "NM_001166357.1",
          "protein_id": "NP_001159829.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 483,
          "cds_start": 86,
          "cds_end": null,
          "cds_length": 1452,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001166357.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.86C>T",
          "hgvs_p": "p.Ser29Leu",
          "transcript": "NM_001166358.2",
          "protein_id": "NP_001159830.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 483,
          "cds_start": 86,
          "cds_end": null,
          "cds_length": 1452,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001166358.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.86C>T",
          "hgvs_p": "p.Ser29Leu",
          "transcript": "NM_001166359.1",
          "protein_id": "NP_001159831.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 483,
          "cds_start": 86,
          "cds_end": null,
          "cds_length": 1452,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001166359.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SHMT2",
          "gene_hgnc_id": 10852,
          "hgvs_c": "c.86C>T",
          "hgvs_p": "p.Ser29Leu",
          "transcript": "ENST00000449049.7",
          "protein_id": "ENSP00000413770.3",
          "transcript_support_level": 2,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 483,
          "cds_start": 86,
          "cds_end": null,
          "cds_length": 1452,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000449049.7"
        },
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        {
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          "transcript": "ENST00000553868.1",
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          "biotype": "retained_intron",
          "feature": "ENST00000553868.1"
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      ],
      "gene_symbol": "SHMT2",
      "gene_hgnc_id": 10852,
      "dbsnp": "rs73338162",
      "frequency_reference_population": 0.011818844,
      "hom_count_reference_population": 304,
      "allele_count_reference_population": 19075,
      "gnomad_exomes_af": 0.0101943,
      "gnomad_genomes_af": 0.027428,
      "gnomad_exomes_ac": 14902,
      "gnomad_genomes_ac": 4173,
      "gnomad_exomes_homalt": 173,
      "gnomad_genomes_homalt": 131,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0030794143676757812,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.033,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0856,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.51,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.379,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BA1",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "NM_005412.6",
          "gene_symbol": "SHMT2",
          "hgnc_id": 10852,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.149C>T",
          "hgvs_p": "p.Ser50Leu"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:2",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}