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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-6548794-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=6548794&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 6548794,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001193457.2",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1136G>A",
          "hgvs_p": "p.Arg379His",
          "transcript": "NM_001193457.2",
          "protein_id": "NP_001180386.1",
          "transcript_support_level": null,
          "aa_start": 379,
          "aa_end": null,
          "aa_length": 571,
          "cds_start": 1136,
          "cds_end": null,
          "cds_length": 1716,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000619571.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001193457.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1136G>A",
          "hgvs_p": "p.Arg379His",
          "transcript": "ENST00000619571.5",
          "protein_id": "ENSP00000482285.1",
          "transcript_support_level": 2,
          "aa_start": 379,
          "aa_end": null,
          "aa_length": 571,
          "cds_start": 1136,
          "cds_end": null,
          "cds_length": 1716,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001193457.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000619571.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1112G>A",
          "hgvs_p": "p.Arg371His",
          "transcript": "ENST00000336604.8",
          "protein_id": "ENSP00000337593.4",
          "transcript_support_level": 1,
          "aa_start": 371,
          "aa_end": null,
          "aa_length": 562,
          "cds_start": 1112,
          "cds_end": null,
          "cds_length": 1689,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000336604.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "n.320G>A",
          "hgvs_p": null,
          "transcript": "ENST00000396830.2",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000396830.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "n.*414G>A",
          "hgvs_p": null,
          "transcript": "ENST00000487279.6",
          "protein_id": "ENSP00000432493.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000487279.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "n.*414G>A",
          "hgvs_p": null,
          "transcript": "ENST00000487279.6",
          "protein_id": "ENSP00000432493.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000487279.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1127G>A",
          "hgvs_p": "p.Arg376His",
          "transcript": "ENST00000900776.1",
          "protein_id": "ENSP00000570835.1",
          "transcript_support_level": null,
          "aa_start": 376,
          "aa_end": null,
          "aa_length": 568,
          "cds_start": 1127,
          "cds_end": null,
          "cds_length": 1707,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000900776.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1112G>A",
          "hgvs_p": "p.Arg371His",
          "transcript": "NM_001039670.3",
          "protein_id": "NP_001034759.1",
          "transcript_support_level": null,
          "aa_start": 371,
          "aa_end": null,
          "aa_length": 563,
          "cds_start": 1112,
          "cds_end": null,
          "cds_length": 1692,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001039670.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1112G>A",
          "hgvs_p": "p.Arg371His",
          "transcript": "ENST00000356896.8",
          "protein_id": "ENSP00000349364.4",
          "transcript_support_level": 5,
          "aa_start": 371,
          "aa_end": null,
          "aa_length": 563,
          "cds_start": 1112,
          "cds_end": null,
          "cds_length": 1692,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000356896.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1112G>A",
          "hgvs_p": "p.Arg371His",
          "transcript": "NM_080730.5",
          "protein_id": "NP_542768.2",
          "transcript_support_level": null,
          "aa_start": 371,
          "aa_end": null,
          "aa_length": 562,
          "cds_start": 1112,
          "cds_end": null,
          "cds_length": 1689,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_080730.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1109G>A",
          "hgvs_p": "p.Arg370His",
          "transcript": "NM_001330325.2",
          "protein_id": "NP_001317254.1",
          "transcript_support_level": null,
          "aa_start": 370,
          "aa_end": null,
          "aa_length": 561,
          "cds_start": 1109,
          "cds_end": null,
          "cds_length": 1686,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001330325.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1103G>A",
          "hgvs_p": "p.Arg368His",
          "transcript": "ENST00000900775.1",
          "protein_id": "ENSP00000570834.1",
          "transcript_support_level": null,
          "aa_start": 368,
          "aa_end": null,
          "aa_length": 560,
          "cds_start": 1103,
          "cds_end": null,
          "cds_length": 1683,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000900775.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1103G>A",
          "hgvs_p": "p.Arg368His",
          "transcript": "NM_001330324.2",
          "protein_id": "NP_001317253.1",
          "transcript_support_level": null,
          "aa_start": 368,
          "aa_end": null,
          "aa_length": 559,
          "cds_start": 1103,
          "cds_end": null,
          "cds_length": 1680,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001330324.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1103G>A",
          "hgvs_p": "p.Arg368His",
          "transcript": "ENST00000396840.6",
          "protein_id": "ENSP00000380052.2",
          "transcript_support_level": 5,
          "aa_start": 368,
          "aa_end": null,
          "aa_length": 559,
          "cds_start": 1103,
          "cds_end": null,
          "cds_length": 1680,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000396840.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1103G>A",
          "hgvs_p": "p.Arg368His",
          "transcript": "ENST00000952553.1",
          "protein_id": "ENSP00000622612.1",
          "transcript_support_level": null,
          "aa_start": 368,
          "aa_end": null,
          "aa_length": 559,
          "cds_start": 1103,
          "cds_end": null,
          "cds_length": 1680,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952553.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.191G>A",
          "hgvs_p": "p.Arg64His",
          "transcript": "ENST00000465801.5",
          "protein_id": "ENSP00000436261.1",
          "transcript_support_level": 5,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 255,
          "cds_start": 191,
          "cds_end": null,
          "cds_length": 768,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000465801.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1136G>A",
          "hgvs_p": "p.Arg379His",
          "transcript": "XM_047428689.1",
          "protein_id": "XP_047284645.1",
          "transcript_support_level": null,
          "aa_start": 379,
          "aa_end": null,
          "aa_length": 659,
          "cds_start": 1136,
          "cds_end": null,
          "cds_length": 1980,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_047428689.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1133G>A",
          "hgvs_p": "p.Arg378His",
          "transcript": "XM_047428690.1",
          "protein_id": "XP_047284646.1",
          "transcript_support_level": null,
          "aa_start": 378,
          "aa_end": null,
          "aa_length": 658,
          "cds_start": 1133,
          "cds_end": null,
          "cds_length": 1977,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047428690.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1112G>A",
          "hgvs_p": "p.Arg371His",
          "transcript": "XM_011520947.4",
          "protein_id": "XP_011519249.1",
          "transcript_support_level": null,
          "aa_start": 371,
          "aa_end": null,
          "aa_length": 651,
          "cds_start": 1112,
          "cds_end": null,
          "cds_length": 1956,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011520947.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IFFO1",
          "gene_hgnc_id": 24970,
          "hgvs_c": "c.1109G>A",
          "hgvs_p": "p.Arg370His",
          "transcript": "XM_011520948.4",
          "protein_id": "XP_011519250.1",
          "transcript_support_level": null,
          "aa_start": 370,
          "aa_end": null,
          "aa_length": 650,
          "cds_start": 1109,
          "cds_end": null,
          "cds_length": 1953,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011520948.4"
        },
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          "biotype": "pseudogene",
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      "gene_symbol": "IFFO1",
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      "dbsnp": "rs149041603",
      "frequency_reference_population": 0.00005887544,
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      "allele_count_reference_population": 95,
      "gnomad_exomes_af": 0.0000602148,
      "gnomad_genomes_af": 0.0000460096,
      "gnomad_exomes_ac": 88,
      "gnomad_genomes_ac": 7,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6159806251525879,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.632,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1847,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.01,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 5.02,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
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          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001193457.2",
          "gene_symbol": "IFFO1",
          "hgnc_id": 24970,
          "effects": [
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          "inheritance_mode": "AR",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}