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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-6860201-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=6860201&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 6860201,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001098536.2",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1181C>T",
          "hgvs_p": "p.Pro394Leu",
          "transcript": "NM_001098536.2",
          "protein_id": "NP_001092006.1",
          "transcript_support_level": null,
          "aa_start": 394,
          "aa_end": null,
          "aa_length": 858,
          "cds_start": 1181,
          "cds_end": null,
          "cds_length": 2577,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000229268.13",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001098536.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1181C>T",
          "hgvs_p": "p.Pro394Leu",
          "transcript": "ENST00000229268.13",
          "protein_id": "ENSP00000229268.8",
          "transcript_support_level": 1,
          "aa_start": 394,
          "aa_end": null,
          "aa_length": 858,
          "cds_start": 1181,
          "cds_end": null,
          "cds_length": 2577,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001098536.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000229268.13"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1181C>T",
          "hgvs_p": "p.Pro394Leu",
          "transcript": "ENST00000389231.9",
          "protein_id": "ENSP00000373883.5",
          "transcript_support_level": 1,
          "aa_start": 394,
          "aa_end": null,
          "aa_length": 835,
          "cds_start": 1181,
          "cds_end": null,
          "cds_length": 2508,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000389231.9"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1181C>T",
          "hgvs_p": "p.Pro394Leu",
          "transcript": "ENST00000864808.1",
          "protein_id": "ENSP00000534867.1",
          "transcript_support_level": null,
          "aa_start": 394,
          "aa_end": null,
          "aa_length": 923,
          "cds_start": 1181,
          "cds_end": null,
          "cds_length": 2772,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000864808.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1181C>T",
          "hgvs_p": "p.Pro394Leu",
          "transcript": "ENST00000864812.1",
          "protein_id": "ENSP00000534871.1",
          "transcript_support_level": null,
          "aa_start": 394,
          "aa_end": null,
          "aa_length": 900,
          "cds_start": 1181,
          "cds_end": null,
          "cds_length": 2703,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000864812.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1193C>T",
          "hgvs_p": "p.Pro398Leu",
          "transcript": "ENST00000864801.1",
          "protein_id": "ENSP00000534859.1",
          "transcript_support_level": null,
          "aa_start": 398,
          "aa_end": null,
          "aa_length": 862,
          "cds_start": 1193,
          "cds_end": null,
          "cds_length": 2589,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000864801.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1181C>T",
          "hgvs_p": "p.Pro394Leu",
          "transcript": "ENST00000864803.1",
          "protein_id": "ENSP00000534862.1",
          "transcript_support_level": null,
          "aa_start": 394,
          "aa_end": null,
          "aa_length": 840,
          "cds_start": 1181,
          "cds_end": null,
          "cds_length": 2523,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000864803.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1193C>T",
          "hgvs_p": "p.Pro398Leu",
          "transcript": "ENST00000864802.1",
          "protein_id": "ENSP00000534860.1",
          "transcript_support_level": null,
          "aa_start": 398,
          "aa_end": null,
          "aa_length": 839,
          "cds_start": 1193,
          "cds_end": null,
          "cds_length": 2520,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000864802.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1181C>T",
          "hgvs_p": "p.Pro394Leu",
          "transcript": "NM_003481.3",
          "protein_id": "NP_003472.2",
          "transcript_support_level": null,
          "aa_start": 394,
          "aa_end": null,
          "aa_length": 835,
          "cds_start": 1181,
          "cds_end": null,
          "cds_length": 2508,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003481.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1109C>T",
          "hgvs_p": "p.Pro370Leu",
          "transcript": "NM_001382591.1",
          "protein_id": "NP_001369520.1",
          "transcript_support_level": null,
          "aa_start": 370,
          "aa_end": null,
          "aa_length": 834,
          "cds_start": 1109,
          "cds_end": null,
          "cds_length": 2505,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001382591.1"
        },
        {
          "aa_ref": "P",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1178C>T",
          "hgvs_p": "p.Pro393Leu",
          "transcript": "ENST00000933307.1",
          "protein_id": "ENSP00000603366.1",
          "transcript_support_level": null,
          "aa_start": 393,
          "aa_end": null,
          "aa_length": 834,
          "cds_start": 1178,
          "cds_end": null,
          "cds_length": 2505,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933307.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1181C>T",
          "hgvs_p": "p.Pro394Leu",
          "transcript": "ENST00000864807.1",
          "protein_id": "ENSP00000534866.1",
          "transcript_support_level": null,
          "aa_start": 394,
          "aa_end": null,
          "aa_length": 830,
          "cds_start": 1181,
          "cds_end": null,
          "cds_length": 2493,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000864807.1"
        },
        {
          "aa_ref": "P",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
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          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1181C>T",
          "hgvs_p": "p.Pro394Leu",
          "transcript": "ENST00000864810.1",
          "protein_id": "ENSP00000534869.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 817,
          "cds_start": 1181,
          "cds_end": null,
          "cds_length": 2454,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000864810.1"
        },
        {
          "aa_ref": "P",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1055C>T",
          "hgvs_p": "p.Pro352Leu",
          "transcript": "NM_001382588.1",
          "protein_id": "NP_001369517.1",
          "transcript_support_level": null,
          "aa_start": 352,
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          "aa_length": 816,
          "cds_start": 1055,
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          "cdna_start": null,
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        },
        {
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          "protein_coding": true,
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          ],
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          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1055C>T",
          "hgvs_p": "p.Pro352Leu",
          "transcript": "ENST00000864811.1",
          "protein_id": "ENSP00000534870.1",
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          "aa_end": null,
          "aa_length": 816,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000864811.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 10,
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          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1181C>T",
          "hgvs_p": "p.Pro394Leu",
          "transcript": "ENST00000864805.1",
          "protein_id": "ENSP00000534864.1",
          "transcript_support_level": null,
          "aa_start": 394,
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          "aa_length": 815,
          "cds_start": 1181,
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        {
          "aa_ref": "P",
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          "strand": true,
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          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1109C>T",
          "hgvs_p": "p.Pro370Leu",
          "transcript": "ENST00000864806.1",
          "protein_id": "ENSP00000534865.1",
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          "aa_start": 370,
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        {
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          "exon_count": 19,
          "intron_rank": null,
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          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1055C>T",
          "hgvs_p": "p.Pro352Leu",
          "transcript": "NM_001382589.1",
          "protein_id": "NP_001369518.1",
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        },
        {
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          ],
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          "gene_symbol": "USP5",
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          "hgvs_c": "c.1055C>T",
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          "transcript": "ENST00000864809.1",
          "protein_id": "ENSP00000534868.1",
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000864809.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP5",
          "gene_hgnc_id": 12628,
          "hgvs_c": "c.1043C>T",
          "hgvs_p": "p.Pro348Leu",
          "transcript": "NM_001382590.1",
          "protein_id": "NP_001369519.1",
          "transcript_support_level": null,
          "aa_start": 348,
          "aa_end": null,
          "aa_length": 789,
          "cds_start": 1043,
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          "cds_length": 2370,
          "cdna_start": null,
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          "cdna_length": null,
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      "dbsnp": "rs782024660",
      "frequency_reference_population": 0.000020521495,
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      "allele_count_reference_population": 33,
      "gnomad_exomes_af": 0.0000199186,
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      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.060139209032058716,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.023,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0628,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.61,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.761,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
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            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001098536.2",
          "gene_symbol": "USP5",
          "hgnc_id": 12628,
          "effects": [
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          "inheritance_mode": "AR",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}