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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-69574737-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=69574737&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 69574737,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000549921.6",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRS2",
          "gene_hgnc_id": 16971,
          "hgvs_c": "c.1309A>G",
          "hgvs_p": "p.Ile437Val",
          "transcript": "NM_001278356.2",
          "protein_id": "NP_001265285.1",
          "transcript_support_level": null,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1309,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": 1712,
          "cdna_end": null,
          "cdna_length": 6768,
          "mane_select": "ENST00000549921.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRS2",
          "gene_hgnc_id": 16971,
          "hgvs_c": "c.1309A>G",
          "hgvs_p": "p.Ile437Val",
          "transcript": "ENST00000549921.6",
          "protein_id": "ENSP00000450048.1",
          "transcript_support_level": 1,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1309,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": 1712,
          "cdna_end": null,
          "cdna_length": 6768,
          "mane_select": "NM_001278356.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRS2",
          "gene_hgnc_id": 16971,
          "hgvs_c": "c.1309A>G",
          "hgvs_p": "p.Ile437Val",
          "transcript": "ENST00000550389.5",
          "protein_id": "ENSP00000447241.1",
          "transcript_support_level": 1,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1309,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": 1555,
          "cdna_end": null,
          "cdna_length": 6600,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRS2",
          "gene_hgnc_id": 16971,
          "hgvs_c": "c.1309A>G",
          "hgvs_p": "p.Ile437Val",
          "transcript": "NM_001042555.3",
          "protein_id": "NP_001036020.1",
          "transcript_support_level": null,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1309,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": 1773,
          "cdna_end": null,
          "cdna_length": 6829,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRS2",
          "gene_hgnc_id": 16971,
          "hgvs_c": "c.1309A>G",
          "hgvs_p": "p.Ile437Val",
          "transcript": "NM_001278351.2",
          "protein_id": "NP_001265280.1",
          "transcript_support_level": null,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1309,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": 1894,
          "cdna_end": null,
          "cdna_length": 6950,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRS2",
          "gene_hgnc_id": 16971,
          "hgvs_c": "c.1309A>G",
          "hgvs_p": "p.Ile437Val",
          "transcript": "NM_001278353.2",
          "protein_id": "NP_001265282.1",
          "transcript_support_level": null,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1309,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": 1837,
          "cdna_end": null,
          "cdna_length": 6893,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRS2",
          "gene_hgnc_id": 16971,
          "hgvs_c": "c.1309A>G",
          "hgvs_p": "p.Ile437Val",
          "transcript": "NM_001278354.2",
          "protein_id": "NP_001265283.1",
          "transcript_support_level": null,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1309,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": 1826,
          "cdna_end": null,
          "cdna_length": 6882,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FRS2",
          "gene_hgnc_id": 16971,
          "hgvs_c": "c.1309A>G",
          "hgvs_p": "p.Ile437Val",
          "transcript": "NM_001278355.2",
          "protein_id": "NP_001265284.1",
          "transcript_support_level": null,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1309,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": 1808,
          "cdna_end": null,
          "cdna_length": 6864,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
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          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
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          "gene_symbol": "FRS2",
          "gene_hgnc_id": 16971,
          "hgvs_c": "c.1309A>G",
          "hgvs_p": "p.Ile437Val",
          "transcript": "NM_001278357.2",
          "protein_id": "NP_001265286.1",
          "transcript_support_level": null,
          "aa_start": 437,
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          "cdna_start": 1573,
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          "mane_select": null,
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        {
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          "protein_coding": true,
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          "consequences": [
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          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "FRS2",
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          "hgvs_c": "c.1309A>G",
          "hgvs_p": "p.Ile437Val",
          "transcript": "NM_006654.5",
          "protein_id": "NP_006645.3",
          "transcript_support_level": null,
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          "hgvs_c": "c.1309A>G",
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          "transcript": "ENST00000397997.6",
          "protein_id": "ENSP00000381083.2",
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          "gene_symbol": "FRS2",
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        {
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        },
        {
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          "gene_symbol": "FRS2",
          "gene_hgnc_id": 16971,
          "hgvs_c": "c.1309A>G",
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          "transcript": "XM_047428123.1",
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        }
      ],
      "gene_symbol": "FRS2",
      "gene_hgnc_id": 16971,
      "dbsnp": "rs777663604",
      "frequency_reference_population": 0.0000034202674,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.00000342027,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 5,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.42240801453590393,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.192,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.4612,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.26,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 9.325,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -3,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4,BS2",
      "acmg_by_gene": [
        {
          "score": -3,
          "benign_score": 5,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000549921.6",
          "gene_symbol": "FRS2",
          "hgnc_id": 16971,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1309A>G",
          "hgvs_p": "p.Ile437Val"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}