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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-81325856-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=81325856&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 81325856,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_003625.5",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2563A>G",
          "hgvs_p": "p.Thr855Ala",
          "transcript": "NM_003625.5",
          "protein_id": "NP_003616.2",
          "transcript_support_level": null,
          "aa_start": 855,
          "aa_end": null,
          "aa_length": 1257,
          "cds_start": 2563,
          "cds_end": null,
          "cds_length": 3774,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000549396.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003625.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2563A>G",
          "hgvs_p": "p.Thr855Ala",
          "transcript": "ENST00000549396.6",
          "protein_id": "ENSP00000450337.1",
          "transcript_support_level": 1,
          "aa_start": 855,
          "aa_end": null,
          "aa_length": 1257,
          "cds_start": 2563,
          "cds_end": null,
          "cds_length": 3774,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_003625.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000549396.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2563A>G",
          "hgvs_p": "p.Thr855Ala",
          "transcript": "ENST00000548586.5",
          "protein_id": "ENSP00000449338.1",
          "transcript_support_level": 1,
          "aa_start": 855,
          "aa_end": null,
          "aa_length": 1251,
          "cds_start": 2563,
          "cds_end": null,
          "cds_length": 3756,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000548586.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2563A>G",
          "hgvs_p": "p.Thr855Ala",
          "transcript": "ENST00000550584.6",
          "protein_id": "ENSP00000449558.2",
          "transcript_support_level": 1,
          "aa_start": 855,
          "aa_end": null,
          "aa_length": 1247,
          "cds_start": 2563,
          "cds_end": null,
          "cds_length": 3744,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000550584.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2563A>G",
          "hgvs_p": "p.Thr855Ala",
          "transcript": "ENST00000552948.5",
          "protein_id": "ENSP00000447868.1",
          "transcript_support_level": 1,
          "aa_start": 855,
          "aa_end": null,
          "aa_length": 1236,
          "cds_start": 2563,
          "cds_end": null,
          "cds_length": 3711,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000552948.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2563A>G",
          "hgvs_p": "p.Thr855Ala",
          "transcript": "NM_001220476.2",
          "protein_id": "NP_001207405.1",
          "transcript_support_level": null,
          "aa_start": 855,
          "aa_end": null,
          "aa_length": 1251,
          "cds_start": 2563,
          "cds_end": null,
          "cds_length": 3756,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001220476.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2563A>G",
          "hgvs_p": "p.Thr855Ala",
          "transcript": "NM_001220473.3",
          "protein_id": "NP_001207402.1",
          "transcript_support_level": null,
          "aa_start": 855,
          "aa_end": null,
          "aa_length": 1247,
          "cds_start": 2563,
          "cds_end": null,
          "cds_length": 3744,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001220473.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2563A>G",
          "hgvs_p": "p.Thr855Ala",
          "transcript": "NM_001220475.2",
          "protein_id": "NP_001207404.1",
          "transcript_support_level": null,
          "aa_start": 855,
          "aa_end": null,
          "aa_length": 1236,
          "cds_start": 2563,
          "cds_end": null,
          "cds_length": 3711,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001220475.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2509A>G",
          "hgvs_p": "p.Thr837Ala",
          "transcript": "NM_001220474.3",
          "protein_id": "NP_001207403.1",
          "transcript_support_level": null,
          "aa_start": 837,
          "aa_end": null,
          "aa_length": 1232,
          "cds_start": 2509,
          "cds_end": null,
          "cds_length": 3699,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001220474.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2509A>G",
          "hgvs_p": "p.Thr837Ala",
          "transcript": "ENST00000549325.5",
          "protein_id": "ENSP00000450298.1",
          "transcript_support_level": 5,
          "aa_start": 837,
          "aa_end": null,
          "aa_length": 1232,
          "cds_start": 2509,
          "cds_end": null,
          "cds_length": 3699,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000549325.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2341A>G",
          "hgvs_p": "p.Thr781Ala",
          "transcript": "NM_001220477.2",
          "protein_id": "NP_001207406.1",
          "transcript_support_level": null,
          "aa_start": 781,
          "aa_end": null,
          "aa_length": 1156,
          "cds_start": 2341,
          "cds_end": null,
          "cds_length": 3471,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001220477.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2341A>G",
          "hgvs_p": "p.Thr781Ala",
          "transcript": "ENST00000407050.8",
          "protein_id": "ENSP00000385093.4",
          "transcript_support_level": 2,
          "aa_start": 781,
          "aa_end": null,
          "aa_length": 1156,
          "cds_start": 2341,
          "cds_end": null,
          "cds_length": 3471,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000407050.8"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2266A>G",
          "hgvs_p": "p.Thr756Ala",
          "transcript": "NM_001220478.2",
          "protein_id": "NP_001207407.1",
          "transcript_support_level": null,
          "aa_start": 756,
          "aa_end": null,
          "aa_length": 1152,
          "cds_start": 2266,
          "cds_end": null,
          "cds_length": 3459,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001220478.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2266A>G",
          "hgvs_p": "p.Thr756Ala",
          "transcript": "ENST00000333447.11",
          "protein_id": "ENSP00000327416.8",
          "transcript_support_level": 5,
          "aa_start": 756,
          "aa_end": null,
          "aa_length": 1152,
          "cds_start": 2266,
          "cds_end": null,
          "cds_length": 3459,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000333447.11"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2266A>G",
          "hgvs_p": "p.Thr756Ala",
          "transcript": "ENST00000443686.7",
          "protein_id": "ENSP00000388373.3",
          "transcript_support_level": 2,
          "aa_start": 756,
          "aa_end": null,
          "aa_length": 1152,
          "cds_start": 2266,
          "cds_end": null,
          "cds_length": 3459,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000443686.7"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2104A>G",
          "hgvs_p": "p.Thr702Ala",
          "transcript": "NM_001282536.1",
          "protein_id": "NP_001269465.1",
          "transcript_support_level": null,
          "aa_start": 702,
          "aa_end": null,
          "aa_length": 1104,
          "cds_start": 2104,
          "cds_end": null,
          "cds_length": 3315,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001282536.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2104A>G",
          "hgvs_p": "p.Thr702Ala",
          "transcript": "ENST00000550359.6",
          "protein_id": "ENSP00000447273.2",
          "transcript_support_level": 2,
          "aa_start": 702,
          "aa_end": null,
          "aa_length": 1104,
          "cds_start": 2104,
          "cds_end": null,
          "cds_length": 3315,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000550359.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.1264A>G",
          "hgvs_p": "p.Thr422Ala",
          "transcript": "NM_001220479.3",
          "protein_id": "NP_001207408.1",
          "transcript_support_level": null,
          "aa_start": 422,
          "aa_end": null,
          "aa_length": 783,
          "cds_start": 1264,
          "cds_end": null,
          "cds_length": 2352,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001220479.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.1264A>G",
          "hgvs_p": "p.Thr422Ala",
          "transcript": "ENST00000541570.6",
          "protein_id": "ENSP00000438337.2",
          "transcript_support_level": 2,
          "aa_start": 422,
          "aa_end": null,
          "aa_length": 783,
          "cds_start": 1264,
          "cds_end": null,
          "cds_length": 2352,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000541570.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.214A>G",
          "hgvs_p": "p.Thr72Ala",
          "transcript": "NM_001220480.3",
          "protein_id": "NP_001207409.1",
          "transcript_support_level": null,
          "aa_start": 72,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 214,
          "cds_end": null,
          "cds_length": 1332,
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          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.2563A>G",
          "hgvs_p": "p.Thr855Ala",
          "transcript": "XM_024449244.2",
          "protein_id": "XP_024305012.1",
          "transcript_support_level": null,
          "aa_start": 855,
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          "aa_length": 887,
          "cds_start": 2563,
          "cds_end": null,
          "cds_length": 2664,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_024449244.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.1339A>G",
          "hgvs_p": "p.Thr447Ala",
          "transcript": "XM_024449245.2",
          "protein_id": "XP_024305013.1",
          "transcript_support_level": null,
          "aa_start": 447,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 1339,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024449245.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "n.*951A>G",
          "hgvs_p": null,
          "transcript": "ENST00000551461.5",
          "protein_id": "ENSP00000449821.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000551461.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "n.*951A>G",
          "hgvs_p": null,
          "transcript": "ENST00000551461.5",
          "protein_id": "ENSP00000449821.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000551461.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "n.1243+48893A>G",
          "hgvs_p": null,
          "transcript": "ENST00000545296.6",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000545296.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPFIA2",
          "gene_hgnc_id": 9246,
          "hgvs_c": "c.-48A>G",
          "hgvs_p": null,
          "transcript": "ENST00000550018.5",
          "protein_id": "ENSP00000447189.1",
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 186,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 561,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000550018.5"
        }
      ],
      "gene_symbol": "PPFIA2",
      "gene_hgnc_id": 9246,
      "dbsnp": "rs745328136",
      "frequency_reference_population": 6.850704e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.8507e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.08188390731811523,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.048,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0715,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.57,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.63,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_003625.5",
          "gene_symbol": "PPFIA2",
          "hgnc_id": 9246,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.2563A>G",
          "hgvs_p": "p.Thr855Ala"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}