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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-89425162-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=89425162&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 89425162,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_172240.3",
      "consequences": [
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1331A>G",
          "hgvs_p": "p.Gln444Arg",
          "transcript": "NM_172240.3",
          "protein_id": "NP_758440.1",
          "transcript_support_level": null,
          "aa_start": 444,
          "aa_end": null,
          "aa_length": 478,
          "cds_start": 1331,
          "cds_end": null,
          "cds_length": 1437,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000313546.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_172240.3"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1331A>G",
          "hgvs_p": "p.Gln444Arg",
          "transcript": "ENST00000313546.8",
          "protein_id": "ENSP00000323302.3",
          "transcript_support_level": 1,
          "aa_start": 444,
          "aa_end": null,
          "aa_length": 478,
          "cds_start": 1331,
          "cds_end": null,
          "cds_length": 1437,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_172240.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000313546.8"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.941A>G",
          "hgvs_p": "p.Gln314Arg",
          "transcript": "ENST00000393179.8",
          "protein_id": "ENSP00000376877.4",
          "transcript_support_level": 1,
          "aa_start": 314,
          "aa_end": null,
          "aa_length": 348,
          "cds_start": 941,
          "cds_end": null,
          "cds_length": 1047,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000393179.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "n.4299A>G",
          "hgvs_p": null,
          "transcript": "ENST00000549591.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000549591.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1271A>G",
          "hgvs_p": "p.Gln424Arg",
          "transcript": "ENST00000928754.1",
          "protein_id": "ENSP00000598813.1",
          "transcript_support_level": null,
          "aa_start": 424,
          "aa_end": null,
          "aa_length": 458,
          "cds_start": 1271,
          "cds_end": null,
          "cds_length": 1377,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928754.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1262A>G",
          "hgvs_p": "p.Gln421Arg",
          "transcript": "ENST00000867695.1",
          "protein_id": "ENSP00000537754.1",
          "transcript_support_level": null,
          "aa_start": 421,
          "aa_end": null,
          "aa_length": 455,
          "cds_start": 1262,
          "cds_end": null,
          "cds_length": 1368,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867695.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1250A>G",
          "hgvs_p": "p.Gln417Arg",
          "transcript": "ENST00000867692.1",
          "protein_id": "ENSP00000537751.1",
          "transcript_support_level": null,
          "aa_start": 417,
          "aa_end": null,
          "aa_length": 451,
          "cds_start": 1250,
          "cds_end": null,
          "cds_length": 1356,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867692.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1223A>G",
          "hgvs_p": "p.Gln408Arg",
          "transcript": "ENST00000928753.1",
          "protein_id": "ENSP00000598812.1",
          "transcript_support_level": null,
          "aa_start": 408,
          "aa_end": null,
          "aa_length": 442,
          "cds_start": 1223,
          "cds_end": null,
          "cds_length": 1329,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928753.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1205A>G",
          "hgvs_p": "p.Gln402Arg",
          "transcript": "NM_001199777.2",
          "protein_id": "NP_001186706.1",
          "transcript_support_level": null,
          "aa_start": 402,
          "aa_end": null,
          "aa_length": 436,
          "cds_start": 1205,
          "cds_end": null,
          "cds_length": 1311,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001199777.2"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1205A>G",
          "hgvs_p": "p.Gln402Arg",
          "transcript": "ENST00000549035.1",
          "protein_id": "ENSP00000447916.1",
          "transcript_support_level": 2,
          "aa_start": 402,
          "aa_end": null,
          "aa_length": 436,
          "cds_start": 1205,
          "cds_end": null,
          "cds_length": 1311,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000549035.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1190A>G",
          "hgvs_p": "p.Gln397Arg",
          "transcript": "ENST00000928752.1",
          "protein_id": "ENSP00000598811.1",
          "transcript_support_level": null,
          "aa_start": 397,
          "aa_end": null,
          "aa_length": 431,
          "cds_start": 1190,
          "cds_end": null,
          "cds_length": 1296,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928752.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1151A>G",
          "hgvs_p": "p.Gln384Arg",
          "transcript": "ENST00000867696.1",
          "protein_id": "ENSP00000537755.1",
          "transcript_support_level": null,
          "aa_start": 384,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 1151,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867696.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 10,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1114-3905A>G",
          "hgvs_p": null,
          "transcript": "ENST00000867693.1",
          "protein_id": "ENSP00000537752.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 405,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1218,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867693.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 10,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1114-20817A>G",
          "hgvs_p": null,
          "transcript": "NM_001425771.1",
          "protein_id": "NP_001412700.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001425771.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 10,
          "intron_rank_end": null,
          "gene_symbol": "POC1B-DUSP6",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1113+34476A>G",
          "hgvs_p": null,
          "transcript": "NM_001425794.1",
          "protein_id": "NP_001412723.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 394,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1185,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001425794.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1045-3905A>G",
          "hgvs_p": null,
          "transcript": "ENST00000867694.1",
          "protein_id": "ENSP00000537753.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 382,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1149,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867694.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1033-3905A>G",
          "hgvs_p": null,
          "transcript": "ENST00000867691.1",
          "protein_id": "ENSP00000537750.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 378,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1137,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867691.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 10,
          "intron_rank_end": null,
          "gene_symbol": "POC1B",
          "gene_hgnc_id": 30836,
          "hgvs_c": "c.1114-23554A>G",
          "hgvs_p": null,
          "transcript": "NM_001425772.1",
          "protein_id": "NP_001412701.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001425772.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "POC1B-DUSP6",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1032+41608A>G",
          "hgvs_p": null,
          "transcript": "NM_001425795.1",
          "protein_id": "NP_001412724.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 367,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1104,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001425795.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "POC1B-DUSP6",
          "gene_hgnc_id": null,
          "hgvs_c": "c.642+41608A>G",
          "hgvs_p": null,
          "transcript": "NM_001425796.1",
          "protein_id": "NP_001412725.1",
          "transcript_support_level": null,
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      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "dbscSNV1_RF",
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      "phylop100way_score": 2.883,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.41,
      "spliceai_max_prediction": "Uncertain_significance",
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      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
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            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_172240.3",
          "gene_symbol": "POC1B",
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          "effects": [
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        {
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            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001425794.1",
          "gene_symbol": "POC1B-DUSP6",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.1113+34476A>G",
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        {
          "score": 3,
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          "criteria": [
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            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000830511.1",
          "gene_symbol": "ENSG00000286608",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.575+377T>C",
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      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}