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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-98544517-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=98544517&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 98544517,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000556029.6",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMPO",
          "gene_hgnc_id": 11875,
          "hgvs_c": "c.859G>C",
          "hgvs_p": "p.Ala287Pro",
          "transcript": "NM_001032283.3",
          "protein_id": "NP_001027454.1",
          "transcript_support_level": null,
          "aa_start": 287,
          "aa_end": null,
          "aa_length": 454,
          "cds_start": 859,
          "cds_end": null,
          "cds_length": 1365,
          "cdna_start": 1137,
          "cdna_end": null,
          "cdna_length": 4136,
          "mane_select": "ENST00000556029.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMPO",
          "gene_hgnc_id": 11875,
          "hgvs_c": "c.859G>C",
          "hgvs_p": "p.Ala287Pro",
          "transcript": "ENST00000556029.6",
          "protein_id": "ENSP00000450627.1",
          "transcript_support_level": 1,
          "aa_start": 287,
          "aa_end": null,
          "aa_length": 454,
          "cds_start": 859,
          "cds_end": null,
          "cds_length": 1365,
          "cdna_start": 1137,
          "cdna_end": null,
          "cdna_length": 4136,
          "mane_select": "NM_001032283.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TMPO",
          "gene_hgnc_id": 11875,
          "hgvs_c": "c.664-1842G>C",
          "hgvs_p": null,
          "transcript": "ENST00000393053.6",
          "protein_id": "ENSP00000376773.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2374,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMPO",
          "gene_hgnc_id": 11875,
          "hgvs_c": "c.859G>C",
          "hgvs_p": "p.Ala287Pro",
          "transcript": "ENST00000715724.1",
          "protein_id": "ENSP00000520506.1",
          "transcript_support_level": null,
          "aa_start": 287,
          "aa_end": null,
          "aa_length": 454,
          "cds_start": 859,
          "cds_end": null,
          "cds_length": 1365,
          "cdna_start": 1166,
          "cdna_end": null,
          "cdna_length": 4165,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMPO",
          "gene_hgnc_id": 11875,
          "hgvs_c": "c.739G>C",
          "hgvs_p": "p.Ala247Pro",
          "transcript": "NM_001307975.2",
          "protein_id": "NP_001294904.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 739,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": 1017,
          "cdna_end": null,
          "cdna_length": 4016,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMPO",
          "gene_hgnc_id": 11875,
          "hgvs_c": "c.739G>C",
          "hgvs_p": "p.Ala247Pro",
          "transcript": "ENST00000343315.9",
          "protein_id": "ENSP00000340251.5",
          "transcript_support_level": 5,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 739,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": 989,
          "cdna_end": null,
          "cdna_length": 2465,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMPO",
          "gene_hgnc_id": 11875,
          "hgvs_c": "n.224G>C",
          "hgvs_p": null,
          "transcript": "ENST00000548223.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 736,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TMPO",
          "gene_hgnc_id": 11875,
          "hgvs_c": "c.664-1842G>C",
          "hgvs_p": null,
          "transcript": "NM_001032284.3",
          "protein_id": "NP_001027455.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3809,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "TMPO",
          "gene_hgnc_id": 11875,
          "hgvs_c": "c.385-434G>C",
          "hgvs_p": null,
          "transcript": "ENST00000556678.1",
          "protein_id": "ENSP00000451552.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 236,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 713,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 713,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TMPO",
          "gene_hgnc_id": 11875,
          "hgvs_c": "c.664-434G>C",
          "hgvs_p": null,
          "transcript": "XM_005269132.5",
          "protein_id": "XP_005269189.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 382,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1149,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3937,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMPO",
          "gene_hgnc_id": 11875,
          "hgvs_c": "n.-122G>C",
          "hgvs_p": null,
          "transcript": "ENST00000551987.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 727,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "TMPO",
      "gene_hgnc_id": 11875,
      "dbsnp": "rs7133258",
      "frequency_reference_population": 0.010104504,
      "hom_count_reference_population": 141,
      "allele_count_reference_population": 16298,
      "gnomad_exomes_af": 0.0102009,
      "gnomad_genomes_af": 0.00917907,
      "gnomad_exomes_ac": 14901,
      "gnomad_genomes_ac": 1397,
      "gnomad_exomes_homalt": 124,
      "gnomad_genomes_homalt": 17,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.003778129816055298,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": null,
      "splice_prediction_selected": null,
      "splice_source_selected": null,
      "revel_score": 0.067,
      "revel_prediction": "Benign",
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.39,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.99,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": null,
      "spliceai_max_prediction": null,
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -16,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -16,
          "benign_score": 16,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000556029.6",
          "gene_symbol": "TMPO",
          "hgnc_id": 11875,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.859G>C",
          "hgvs_p": "p.Ala287Pro"
        }
      ],
      "clinvar_disease": "TMPO-related disorder,not provided,not specified",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:5",
      "phenotype_combined": "not specified|not provided|TMPO-related disorder",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}