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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 13-100449297-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=13&pos=100449297&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "13",
      "pos": 100449297,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_000282.4",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1891G>C",
          "hgvs_p": "p.Gly631Arg",
          "transcript": "NM_000282.4",
          "protein_id": "NP_000273.2",
          "transcript_support_level": null,
          "aa_start": 631,
          "aa_end": null,
          "aa_length": 728,
          "cds_start": 1891,
          "cds_end": null,
          "cds_length": 2187,
          "cdna_start": 1919,
          "cdna_end": null,
          "cdna_length": 2484,
          "mane_select": "ENST00000376285.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000282.4"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1891G>C",
          "hgvs_p": "p.Gly631Arg",
          "transcript": "ENST00000376285.6",
          "protein_id": "ENSP00000365462.1",
          "transcript_support_level": 1,
          "aa_start": 631,
          "aa_end": null,
          "aa_length": 728,
          "cds_start": 1891,
          "cds_end": null,
          "cds_length": 2187,
          "cdna_start": 1919,
          "cdna_end": null,
          "cdna_length": 2484,
          "mane_select": "NM_000282.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000376285.6"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.2014G>C",
          "hgvs_p": "p.Gly672Arg",
          "transcript": "ENST00000881637.1",
          "protein_id": "ENSP00000551696.1",
          "transcript_support_level": null,
          "aa_start": 672,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 2014,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": 2039,
          "cdna_end": null,
          "cdna_length": 2604,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881637.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1996G>C",
          "hgvs_p": "p.Gly666Arg",
          "transcript": "ENST00000881640.1",
          "protein_id": "ENSP00000551699.1",
          "transcript_support_level": null,
          "aa_start": 666,
          "aa_end": null,
          "aa_length": 763,
          "cds_start": 1996,
          "cds_end": null,
          "cds_length": 2292,
          "cdna_start": 2024,
          "cdna_end": null,
          "cdna_length": 2586,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881640.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1891G>C",
          "hgvs_p": "p.Gly631Arg",
          "transcript": "ENST00000956821.1",
          "protein_id": "ENSP00000626880.1",
          "transcript_support_level": null,
          "aa_start": 631,
          "aa_end": null,
          "aa_length": 762,
          "cds_start": 1891,
          "cds_end": null,
          "cds_length": 2289,
          "cdna_start": 1914,
          "cdna_end": null,
          "cdna_length": 2577,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956821.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1981G>C",
          "hgvs_p": "p.Gly661Arg",
          "transcript": "ENST00000881647.1",
          "protein_id": "ENSP00000551706.1",
          "transcript_support_level": null,
          "aa_start": 661,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 1981,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": 1989,
          "cdna_end": null,
          "cdna_length": 2550,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881647.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1966G>C",
          "hgvs_p": "p.Gly656Arg",
          "transcript": "ENST00000881631.1",
          "protein_id": "ENSP00000551690.1",
          "transcript_support_level": null,
          "aa_start": 656,
          "aa_end": null,
          "aa_length": 753,
          "cds_start": 1966,
          "cds_end": null,
          "cds_length": 2262,
          "cdna_start": 1994,
          "cdna_end": null,
          "cdna_length": 2559,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881631.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.2014G>C",
          "hgvs_p": "p.Gly672Arg",
          "transcript": "ENST00000881644.1",
          "protein_id": "ENSP00000551703.1",
          "transcript_support_level": null,
          "aa_start": 672,
          "aa_end": null,
          "aa_length": 738,
          "cds_start": 2014,
          "cds_end": null,
          "cds_length": 2217,
          "cdna_start": 2024,
          "cdna_end": null,
          "cdna_length": 2492,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881644.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1891G>C",
          "hgvs_p": "p.Gly631Arg",
          "transcript": "ENST00000940854.1",
          "protein_id": "ENSP00000610913.1",
          "transcript_support_level": null,
          "aa_start": 631,
          "aa_end": null,
          "aa_length": 726,
          "cds_start": 1891,
          "cds_end": null,
          "cds_length": 2181,
          "cdna_start": 1897,
          "cdna_end": null,
          "cdna_length": 2453,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940854.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1879G>C",
          "hgvs_p": "p.Gly627Arg",
          "transcript": "ENST00000881635.1",
          "protein_id": "ENSP00000551694.1",
          "transcript_support_level": null,
          "aa_start": 627,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1879,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": 1904,
          "cdna_end": null,
          "cdna_length": 2469,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881635.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1837G>C",
          "hgvs_p": "p.Gly613Arg",
          "transcript": "ENST00000956823.1",
          "protein_id": "ENSP00000626882.1",
          "transcript_support_level": null,
          "aa_start": 613,
          "aa_end": null,
          "aa_length": 710,
          "cds_start": 1837,
          "cds_end": null,
          "cds_length": 2133,
          "cdna_start": 1847,
          "cdna_end": null,
          "cdna_length": 2409,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956823.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1822G>C",
          "hgvs_p": "p.Gly608Arg",
          "transcript": "ENST00000956827.1",
          "protein_id": "ENSP00000626886.1",
          "transcript_support_level": null,
          "aa_start": 608,
          "aa_end": null,
          "aa_length": 705,
          "cds_start": 1822,
          "cds_end": null,
          "cds_length": 2118,
          "cdna_start": 1826,
          "cdna_end": null,
          "cdna_length": 2387,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956827.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1813G>C",
          "hgvs_p": "p.Gly605Arg",
          "transcript": "NM_001127692.3",
          "protein_id": "NP_001121164.1",
          "transcript_support_level": null,
          "aa_start": 605,
          "aa_end": null,
          "aa_length": 702,
          "cds_start": 1813,
          "cds_end": null,
          "cds_length": 2109,
          "cdna_start": 1841,
          "cdna_end": null,
          "cdna_length": 2406,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001127692.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1813G>C",
          "hgvs_p": "p.Gly605Arg",
          "transcript": "ENST00000376286.8",
          "protein_id": "ENSP00000365463.4",
          "transcript_support_level": 2,
          "aa_start": 605,
          "aa_end": null,
          "aa_length": 702,
          "cds_start": 1813,
          "cds_end": null,
          "cds_length": 2109,
          "cdna_start": 1919,
          "cdna_end": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000376286.8"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1891G>C",
          "hgvs_p": "p.Gly631Arg",
          "transcript": "ENST00000881634.1",
          "protein_id": "ENSP00000551693.1",
          "transcript_support_level": null,
          "aa_start": 631,
          "aa_end": null,
          "aa_length": 697,
          "cds_start": 1891,
          "cds_end": null,
          "cds_length": 2094,
          "cdna_start": 1919,
          "cdna_end": null,
          "cdna_length": 2388,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881634.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1792G>C",
          "hgvs_p": "p.Gly598Arg",
          "transcript": "ENST00000881627.1",
          "protein_id": "ENSP00000551686.1",
          "transcript_support_level": null,
          "aa_start": 598,
          "aa_end": null,
          "aa_length": 695,
          "cds_start": 1792,
          "cds_end": null,
          "cds_length": 2088,
          "cdna_start": 1830,
          "cdna_end": null,
          "cdna_length": 2395,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881627.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1780G>C",
          "hgvs_p": "p.Gly594Arg",
          "transcript": "ENST00000881645.1",
          "protein_id": "ENSP00000551704.1",
          "transcript_support_level": null,
          "aa_start": 594,
          "aa_end": null,
          "aa_length": 691,
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          "cds_end": null,
          "cds_length": 2076,
          "cdna_start": 1790,
          "cdna_end": null,
          "cdna_length": 2351,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000881645.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1774G>C",
          "hgvs_p": "p.Gly592Arg",
          "transcript": "ENST00000881638.1",
          "protein_id": "ENSP00000551697.1",
          "transcript_support_level": null,
          "aa_start": 592,
          "aa_end": null,
          "aa_length": 689,
          "cds_start": 1774,
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          "cds_length": 2070,
          "cdna_start": 1802,
          "cdna_end": null,
          "cdna_length": 2364,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000881638.1"
        },
        {
          "aa_ref": "G",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1672G>C",
          "hgvs_p": "p.Gly558Arg",
          "transcript": "ENST00000956825.1",
          "protein_id": "ENSP00000626884.1",
          "transcript_support_level": null,
          "aa_start": 558,
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          "cds_start": 1672,
          "cds_end": null,
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          "cdna_start": 1677,
          "cdna_end": null,
          "cdna_length": 2342,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956825.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1759G>C",
          "hgvs_p": "p.Gly587Arg",
          "transcript": "ENST00000881626.1",
          "protein_id": "ENSP00000551685.1",
          "transcript_support_level": null,
          "aa_start": 587,
          "aa_end": null,
          "aa_length": 684,
          "cds_start": 1759,
          "cds_end": null,
          "cds_length": 2055,
          "cdna_start": 1797,
          "cdna_end": null,
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          "biotype": "pseudogene",
          "feature": "XR_007063681.1"
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        {
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          "canonical": false,
          "protein_coding": false,
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          "consequences": [
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          "exon_count": 19,
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          "gene_symbol": "PCCA",
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          "hgvs_c": "n.*426G>C",
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          "transcript": "ENST00000636475.1",
          "protein_id": "ENSP00000490843.1",
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          "aa_start": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 1971,
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000636475.1"
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        {
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            "3_prime_UTR_variant"
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          "exon_count": 21,
          "intron_rank": null,
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          "gene_symbol": "PCCA",
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          "hgvs_c": "n.*1375G>C",
          "hgvs_p": null,
          "transcript": "ENST00000647303.1",
          "protein_id": "ENSP00000495663.1",
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          "aa_start": null,
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          "cds_length": null,
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          "cdna_length": 2196,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000647303.1"
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      ],
      "gene_symbol": "PCCA",
      "gene_hgnc_id": 8653,
      "dbsnp": "rs796052018",
      "frequency_reference_population": 0.0000029184298,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 4,
      "gnomad_exomes_af": 0.00000291843,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9674254655838013,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.843,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9842,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.54,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 6.979,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 12,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PS3,PM2,PP3_Strong,PP5_Moderate",
      "acmg_by_gene": [
        {
          "score": 12,
          "benign_score": 0,
          "pathogenic_score": 12,
          "criteria": [
            "PS3",
            "PM2",
            "PP3_Strong",
            "PP5_Moderate"
          ],
          "verdict": "Pathogenic",
          "transcript": "NM_000282.4",
          "gene_symbol": "PCCA",
          "hgnc_id": 8653,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1891G>C",
          "hgvs_p": "p.Gly631Arg"
        }
      ],
      "clinvar_disease": "Propionic acidemia",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "phenotype_combined": "Propionic acidemia",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}
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