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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 13-100515524-T-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=13&pos=100515524&ref=T&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "13",
      "pos": 100515524,
      "ref": "T",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_000282.4",
      "consequences": [
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1997T>A",
          "hgvs_p": "p.Met666Lys",
          "transcript": "NM_000282.4",
          "protein_id": "NP_000273.2",
          "transcript_support_level": null,
          "aa_start": 666,
          "aa_end": null,
          "aa_length": 728,
          "cds_start": 1997,
          "cds_end": null,
          "cds_length": 2187,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000376285.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000282.4"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1997T>A",
          "hgvs_p": "p.Met666Lys",
          "transcript": "ENST00000376285.6",
          "protein_id": "ENSP00000365462.1",
          "transcript_support_level": 1,
          "aa_start": 666,
          "aa_end": null,
          "aa_length": 728,
          "cds_start": 1997,
          "cds_end": null,
          "cds_length": 2187,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000282.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000376285.6"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.2120T>A",
          "hgvs_p": "p.Met707Lys",
          "transcript": "ENST00000881637.1",
          "protein_id": "ENSP00000551696.1",
          "transcript_support_level": null,
          "aa_start": 707,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 2120,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881637.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.2102T>A",
          "hgvs_p": "p.Met701Lys",
          "transcript": "ENST00000881640.1",
          "protein_id": "ENSP00000551699.1",
          "transcript_support_level": null,
          "aa_start": 701,
          "aa_end": null,
          "aa_length": 763,
          "cds_start": 2102,
          "cds_end": null,
          "cds_length": 2292,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881640.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.2099T>A",
          "hgvs_p": "p.Met700Lys",
          "transcript": "ENST00000956821.1",
          "protein_id": "ENSP00000626880.1",
          "transcript_support_level": null,
          "aa_start": 700,
          "aa_end": null,
          "aa_length": 762,
          "cds_start": 2099,
          "cds_end": null,
          "cds_length": 2289,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956821.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.2087T>A",
          "hgvs_p": "p.Met696Lys",
          "transcript": "ENST00000881647.1",
          "protein_id": "ENSP00000551706.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881647.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.2072T>A",
          "hgvs_p": "p.Met691Lys",
          "transcript": "ENST00000881631.1",
          "protein_id": "ENSP00000551690.1",
          "transcript_support_level": null,
          "aa_start": 691,
          "aa_end": null,
          "aa_length": 753,
          "cds_start": 2072,
          "cds_end": null,
          "cds_length": 2262,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881631.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1991T>A",
          "hgvs_p": "p.Met664Lys",
          "transcript": "ENST00000940854.1",
          "protein_id": "ENSP00000610913.1",
          "transcript_support_level": null,
          "aa_start": 664,
          "aa_end": null,
          "aa_length": 726,
          "cds_start": 1991,
          "cds_end": null,
          "cds_length": 2181,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940854.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1985T>A",
          "hgvs_p": "p.Met662Lys",
          "transcript": "ENST00000881635.1",
          "protein_id": "ENSP00000551694.1",
          "transcript_support_level": null,
          "aa_start": 662,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1985,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881635.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1943T>A",
          "hgvs_p": "p.Met648Lys",
          "transcript": "NM_001352605.2",
          "protein_id": "NP_001339534.1",
          "transcript_support_level": null,
          "aa_start": 648,
          "aa_end": null,
          "aa_length": 710,
          "cds_start": 1943,
          "cds_end": null,
          "cds_length": 2133,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001352605.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1943T>A",
          "hgvs_p": "p.Met648Lys",
          "transcript": "ENST00000881625.1",
          "protein_id": "ENSP00000551684.1",
          "transcript_support_level": null,
          "aa_start": 648,
          "aa_end": null,
          "aa_length": 710,
          "cds_start": 1943,
          "cds_end": null,
          "cds_length": 2133,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881625.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1943T>A",
          "hgvs_p": "p.Met648Lys",
          "transcript": "ENST00000956823.1",
          "protein_id": "ENSP00000626882.1",
          "transcript_support_level": null,
          "aa_start": 648,
          "aa_end": null,
          "aa_length": 710,
          "cds_start": 1943,
          "cds_end": null,
          "cds_length": 2133,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956823.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1928T>A",
          "hgvs_p": "p.Met643Lys",
          "transcript": "ENST00000956827.1",
          "protein_id": "ENSP00000626886.1",
          "transcript_support_level": null,
          "aa_start": 643,
          "aa_end": null,
          "aa_length": 705,
          "cds_start": 1928,
          "cds_end": null,
          "cds_length": 2118,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956827.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1919T>A",
          "hgvs_p": "p.Met640Lys",
          "transcript": "NM_001127692.3",
          "protein_id": "NP_001121164.1",
          "transcript_support_level": null,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 702,
          "cds_start": 1919,
          "cds_end": null,
          "cds_length": 2109,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001127692.3"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1919T>A",
          "hgvs_p": "p.Met640Lys",
          "transcript": "ENST00000376286.8",
          "protein_id": "ENSP00000365463.4",
          "transcript_support_level": 2,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 702,
          "cds_start": 1919,
          "cds_end": null,
          "cds_length": 2109,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000376286.8"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1898T>A",
          "hgvs_p": "p.Met633Lys",
          "transcript": "ENST00000881627.1",
          "protein_id": "ENSP00000551686.1",
          "transcript_support_level": null,
          "aa_start": 633,
          "aa_end": null,
          "aa_length": 695,
          "cds_start": 1898,
          "cds_end": null,
          "cds_length": 2088,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881627.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1886T>A",
          "hgvs_p": "p.Met629Lys",
          "transcript": "ENST00000881645.1",
          "protein_id": "ENSP00000551704.1",
          "transcript_support_level": null,
          "aa_start": 629,
          "aa_end": null,
          "aa_length": 691,
          "cds_start": 1886,
          "cds_end": null,
          "cds_length": 2076,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881645.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1880T>A",
          "hgvs_p": "p.Met627Lys",
          "transcript": "ENST00000881638.1",
          "protein_id": "ENSP00000551697.1",
          "transcript_support_level": null,
          "aa_start": 627,
          "aa_end": null,
          "aa_length": 689,
          "cds_start": 1880,
          "cds_end": null,
          "cds_length": 2070,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881638.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1880T>A",
          "hgvs_p": "p.Met627Lys",
          "transcript": "ENST00000956825.1",
          "protein_id": "ENSP00000626884.1",
          "transcript_support_level": null,
          "aa_start": 627,
          "aa_end": null,
          "aa_length": 689,
          "cds_start": 1880,
          "cds_end": null,
          "cds_length": 2070,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956825.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1865T>A",
          "hgvs_p": "p.Met622Lys",
          "transcript": "ENST00000881626.1",
          "protein_id": "ENSP00000551685.1",
          "transcript_support_level": null,
          "aa_start": 622,
          "aa_end": null,
          "aa_length": 684,
          "cds_start": 1865,
          "cds_end": null,
          "cds_length": 2055,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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      "gene_symbol": "PCCA",
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      "dbsnp": "rs999241357",
      "frequency_reference_population": 0.000004956525,
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      "gnomad_exomes_af": 0.00000410447,
      "gnomad_genomes_af": 0.0000131396,
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      "gnomad_exomes_homalt": 0,
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      "gnomad_mito_homoplasmic": null,
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      "computational_score_selected": 0.9857579469680786,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.879,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9857,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.33,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.645,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
      "apogee2_prediction": null,
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      "mitotip_prediction": null,
      "acmg_score": 7,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong,PP5",
      "acmg_by_gene": [
        {
          "score": 7,
          "benign_score": 0,
          "pathogenic_score": 7,
          "criteria": [
            "PM2",
            "PP3_Strong",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_000282.4",
          "gene_symbol": "PCCA",
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          "effects": [
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          "inheritance_mode": "AR",
          "hgvs_c": "c.1997T>A",
          "hgvs_p": "p.Met666Lys"
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      ],
      "clinvar_disease": "Propionic acidemia,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "LP:1 US:1",
      "phenotype_combined": "Propionic acidemia|not specified",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}