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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 13-100515544-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=13&pos=100515544&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "13",
      "pos": 100515544,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000376285.6",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.2017G>A",
          "hgvs_p": "p.Val673Ile",
          "transcript": "NM_000282.4",
          "protein_id": "NP_000273.2",
          "transcript_support_level": null,
          "aa_start": 673,
          "aa_end": null,
          "aa_length": 728,
          "cds_start": 2017,
          "cds_end": null,
          "cds_length": 2187,
          "cdna_start": 2045,
          "cdna_end": null,
          "cdna_length": 2484,
          "mane_select": "ENST00000376285.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.2017G>A",
          "hgvs_p": "p.Val673Ile",
          "transcript": "ENST00000376285.6",
          "protein_id": "ENSP00000365462.1",
          "transcript_support_level": 1,
          "aa_start": 673,
          "aa_end": null,
          "aa_length": 728,
          "cds_start": 2017,
          "cds_end": null,
          "cds_length": 2187,
          "cdna_start": 2045,
          "cdna_end": null,
          "cdna_length": 2484,
          "mane_select": "NM_000282.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1963G>A",
          "hgvs_p": "p.Val655Ile",
          "transcript": "NM_001352605.2",
          "protein_id": "NP_001339534.1",
          "transcript_support_level": null,
          "aa_start": 655,
          "aa_end": null,
          "aa_length": 710,
          "cds_start": 1963,
          "cds_end": null,
          "cds_length": 2133,
          "cdna_start": 1991,
          "cdna_end": null,
          "cdna_length": 2430,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1939G>A",
          "hgvs_p": "p.Val647Ile",
          "transcript": "NM_001127692.3",
          "protein_id": "NP_001121164.1",
          "transcript_support_level": null,
          "aa_start": 647,
          "aa_end": null,
          "aa_length": 702,
          "cds_start": 1939,
          "cds_end": null,
          "cds_length": 2109,
          "cdna_start": 1967,
          "cdna_end": null,
          "cdna_length": 2406,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1939G>A",
          "hgvs_p": "p.Val647Ile",
          "transcript": "ENST00000376286.8",
          "protein_id": "ENSP00000365463.4",
          "transcript_support_level": 2,
          "aa_start": 647,
          "aa_end": null,
          "aa_length": 702,
          "cds_start": 1939,
          "cds_end": null,
          "cds_length": 2109,
          "cdna_start": 2045,
          "cdna_end": null,
          "cdna_length": 2481,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1873G>A",
          "hgvs_p": "p.Val625Ile",
          "transcript": "NM_001352606.2",
          "protein_id": "NP_001339535.1",
          "transcript_support_level": null,
          "aa_start": 625,
          "aa_end": null,
          "aa_length": 680,
          "cds_start": 1873,
          "cds_end": null,
          "cds_length": 2043,
          "cdna_start": 1901,
          "cdna_end": null,
          "cdna_length": 2340,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1795G>A",
          "hgvs_p": "p.Val599Ile",
          "transcript": "NM_001352608.2",
          "protein_id": "NP_001339537.1",
          "transcript_support_level": null,
          "aa_start": 599,
          "aa_end": null,
          "aa_length": 654,
          "cds_start": 1795,
          "cds_end": null,
          "cds_length": 1965,
          "cdna_start": 1823,
          "cdna_end": null,
          "cdna_length": 2262,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1675G>A",
          "hgvs_p": "p.Val559Ile",
          "transcript": "ENST00000637657.1",
          "protein_id": "ENSP00000489974.1",
          "transcript_support_level": 5,
          "aa_start": 559,
          "aa_end": null,
          "aa_length": 614,
          "cds_start": 1675,
          "cds_end": null,
          "cds_length": 1845,
          "cdna_start": 1677,
          "cdna_end": null,
          "cdna_length": 2118,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1213G>A",
          "hgvs_p": "p.Val405Ile",
          "transcript": "ENST00000636366.1",
          "protein_id": "ENSP00000490939.1",
          "transcript_support_level": 5,
          "aa_start": 405,
          "aa_end": null,
          "aa_length": 460,
          "cds_start": 1213,
          "cds_end": null,
          "cds_length": 1383,
          "cdna_start": 1215,
          "cdna_end": null,
          "cdna_length": 1654,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1072G>A",
          "hgvs_p": "p.Val358Ile",
          "transcript": "NM_001352610.2",
          "protein_id": "NP_001339539.1",
          "transcript_support_level": null,
          "aa_start": 358,
          "aa_end": null,
          "aa_length": 413,
          "cds_start": 1072,
          "cds_end": null,
          "cds_length": 1242,
          "cdna_start": 1966,
          "cdna_end": null,
          "cdna_length": 2405,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1018G>A",
          "hgvs_p": "p.Val340Ile",
          "transcript": "NM_001352611.2",
          "protein_id": "NP_001339540.1",
          "transcript_support_level": null,
          "aa_start": 340,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": 1018,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": 1912,
          "cdna_end": null,
          "cdna_length": 2351,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.928G>A",
          "hgvs_p": "p.Val310Ile",
          "transcript": "NM_001352612.2",
          "protein_id": "NP_001339541.1",
          "transcript_support_level": null,
          "aa_start": 310,
          "aa_end": null,
          "aa_length": 365,
          "cds_start": 928,
          "cds_end": null,
          "cds_length": 1098,
          "cdna_start": 1822,
          "cdna_end": null,
          "cdna_length": 2261,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.118G>A",
          "hgvs_p": "p.Val40Ile",
          "transcript": "ENST00000428969.1",
          "protein_id": "ENSP00000399413.1",
          "transcript_support_level": 3,
          "aa_start": 40,
          "aa_end": null,
          "aa_length": 111,
          "cds_start": 118,
          "cds_end": null,
          "cds_length": 336,
          "cdna_start": 118,
          "cdna_end": null,
          "cdna_length": 602,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1918G>A",
          "hgvs_p": "p.Val640Ile",
          "transcript": "XM_017020609.2",
          "protein_id": "XP_016876098.1",
          "transcript_support_level": null,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 695,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 2088,
          "cdna_start": 3085,
          "cdna_end": null,
          "cdna_length": 3524,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "c.1885G>A",
          "hgvs_p": "p.Val629Ile",
          "transcript": "XM_047430371.1",
          "protein_id": "XP_047286327.1",
          "transcript_support_level": null,
          "aa_start": 629,
          "aa_end": null,
          "aa_length": 684,
          "cds_start": 1885,
          "cds_end": null,
          "cds_length": 2055,
          "cdna_start": 1913,
          "cdna_end": null,
          "cdna_length": 2352,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "n.*552G>A",
          "hgvs_p": null,
          "transcript": "ENST00000636475.1",
          "protein_id": "ENSP00000490843.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1971,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "n.*1501G>A",
          "hgvs_p": null,
          "transcript": "ENST00000647303.1",
          "protein_id": "ENSP00000495663.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2196,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "n.2026G>A",
          "hgvs_p": null,
          "transcript": "NR_148028.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2465,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "n.1948G>A",
          "hgvs_p": null,
          "transcript": "NR_148029.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2387,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "n.2129G>A",
          "hgvs_p": null,
          "transcript": "NR_148030.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2568,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PCCA",
          "gene_hgnc_id": 8653,
          "hgvs_c": "n.1942G>A",
          "hgvs_p": null,
          "transcript": "NR_148031.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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      ],
      "gene_symbol": "PCCA",
      "gene_hgnc_id": 8653,
      "dbsnp": "rs142646074",
      "frequency_reference_population": 0.00001920732,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 31,
      "gnomad_exomes_af": 0.0000171037,
      "gnomad_genomes_af": 0.0000393975,
      "gnomad_exomes_ac": 25,
      "gnomad_genomes_ac": 6,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.04599180817604065,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.153,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.064,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.47,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.817,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM1,PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 4,
          "pathogenic_score": 4,
          "criteria": [
            "PM1",
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000376285.6",
          "gene_symbol": "PCCA",
          "hgnc_id": 8653,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2017G>A",
          "hgvs_p": "p.Val673Ile"
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      ],
      "clinvar_disease": "Propionic acidemia,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "Propionic acidemia|not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}