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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 13-109009011-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=13&pos=109009011&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "13",
      "pos": 109009011,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000457511.7",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYO16",
          "gene_hgnc_id": 29822,
          "hgvs_c": "c.2557C>G",
          "hgvs_p": "p.Pro853Ala",
          "transcript": "NM_001198950.3",
          "protein_id": "NP_001185879.1",
          "transcript_support_level": null,
          "aa_start": 853,
          "aa_end": null,
          "aa_length": 1880,
          "cds_start": 2557,
          "cds_end": null,
          "cds_length": 5643,
          "cdna_start": 2791,
          "cdna_end": null,
          "cdna_length": 7046,
          "mane_select": "ENST00000457511.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYO16",
          "gene_hgnc_id": 29822,
          "hgvs_c": "c.2557C>G",
          "hgvs_p": "p.Pro853Ala",
          "transcript": "ENST00000457511.7",
          "protein_id": "ENSP00000401633.3",
          "transcript_support_level": 1,
          "aa_start": 853,
          "aa_end": null,
          "aa_length": 1880,
          "cds_start": 2557,
          "cds_end": null,
          "cds_length": 5643,
          "cdna_start": 2791,
          "cdna_end": null,
          "cdna_length": 7046,
          "mane_select": "NM_001198950.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYO16",
          "gene_hgnc_id": 29822,
          "hgvs_c": "c.2491C>G",
          "hgvs_p": "p.Pro831Ala",
          "transcript": "ENST00000356711.7",
          "protein_id": "ENSP00000349145.2",
          "transcript_support_level": 1,
          "aa_start": 831,
          "aa_end": null,
          "aa_length": 1858,
          "cds_start": 2491,
          "cds_end": null,
          "cds_length": 5577,
          "cdna_start": 2617,
          "cdna_end": null,
          "cdna_length": 6864,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYO16",
          "gene_hgnc_id": 29822,
          "hgvs_c": "c.2491C>G",
          "hgvs_p": "p.Pro831Ala",
          "transcript": "NM_015011.3",
          "protein_id": "NP_055826.1",
          "transcript_support_level": null,
          "aa_start": 831,
          "aa_end": null,
          "aa_length": 1858,
          "cds_start": 2491,
          "cds_end": null,
          "cds_length": 5577,
          "cdna_start": 2864,
          "cdna_end": null,
          "cdna_length": 7119,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYO16",
          "gene_hgnc_id": 29822,
          "hgvs_c": "c.2491C>G",
          "hgvs_p": "p.Pro831Ala",
          "transcript": "ENST00000251041.10",
          "protein_id": "ENSP00000251041.5",
          "transcript_support_level": 5,
          "aa_start": 831,
          "aa_end": null,
          "aa_length": 940,
          "cds_start": 2491,
          "cds_end": null,
          "cds_length": 2823,
          "cdna_start": 2587,
          "cdna_end": null,
          "cdna_length": 3547,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYO16",
          "gene_hgnc_id": 29822,
          "hgvs_c": "c.2554C>G",
          "hgvs_p": "p.Pro852Ala",
          "transcript": "XM_047430182.1",
          "protein_id": "XP_047286138.1",
          "transcript_support_level": null,
          "aa_start": 852,
          "aa_end": null,
          "aa_length": 1879,
          "cds_start": 2554,
          "cds_end": null,
          "cds_length": 5640,
          "cdna_start": 2788,
          "cdna_end": null,
          "cdna_length": 7043,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYO16",
          "gene_hgnc_id": 29822,
          "hgvs_c": "c.2491C>G",
          "hgvs_p": "p.Pro831Ala",
          "transcript": "XM_011521062.2",
          "protein_id": "XP_011519364.1",
          "transcript_support_level": null,
          "aa_start": 831,
          "aa_end": null,
          "aa_length": 1858,
          "cds_start": 2491,
          "cds_end": null,
          "cds_length": 5577,
          "cdna_start": 2738,
          "cdna_end": null,
          "cdna_length": 6993,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYO16",
          "gene_hgnc_id": 29822,
          "hgvs_c": "c.2488C>G",
          "hgvs_p": "p.Pro830Ala",
          "transcript": "XM_047430183.1",
          "protein_id": "XP_047286139.1",
          "transcript_support_level": null,
          "aa_start": 830,
          "aa_end": null,
          "aa_length": 1857,
          "cds_start": 2488,
          "cds_end": null,
          "cds_length": 5574,
          "cdna_start": 2735,
          "cdna_end": null,
          "cdna_length": 6990,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYO16",
          "gene_hgnc_id": 29822,
          "hgvs_c": "c.1027C>G",
          "hgvs_p": "p.Pro343Ala",
          "transcript": "XM_047430184.1",
          "protein_id": "XP_047286140.1",
          "transcript_support_level": null,
          "aa_start": 343,
          "aa_end": null,
          "aa_length": 1370,
          "cds_start": 1027,
          "cds_end": null,
          "cds_length": 4113,
          "cdna_start": 1405,
          "cdna_end": null,
          "cdna_length": 5660,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYO16",
          "gene_hgnc_id": 29822,
          "hgvs_c": "c.646C>G",
          "hgvs_p": "p.Pro216Ala",
          "transcript": "XM_047430185.1",
          "protein_id": "XP_047286141.1",
          "transcript_support_level": null,
          "aa_start": 216,
          "aa_end": null,
          "aa_length": 1243,
          "cds_start": 646,
          "cds_end": null,
          "cds_length": 3732,
          "cdna_start": 765,
          "cdna_end": null,
          "cdna_length": 5020,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYO16",
          "gene_hgnc_id": 29822,
          "hgvs_c": "n.1877C>G",
          "hgvs_p": null,
          "transcript": "ENST00000375857.6",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3117,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "MYO16",
      "gene_hgnc_id": 29822,
      "dbsnp": "rs3825491",
      "frequency_reference_population": 0.39273515,
      "hom_count_reference_population": 126969,
      "allele_count_reference_population": 627773,
      "gnomad_exomes_af": 0.393333,
      "gnomad_genomes_af": 0.387049,
      "gnomad_exomes_ac": 568933,
      "gnomad_genomes_ac": 58840,
      "gnomad_exomes_homalt": 115233,
      "gnomad_genomes_homalt": 11736,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.00009509406663710251,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.441,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.0811,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.2,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.925,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -13,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6,BA1",
      "acmg_by_gene": [
        {
          "score": -13,
          "benign_score": 13,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000457511.7",
          "gene_symbol": "MYO16",
          "hgnc_id": 29822,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2557C>G",
          "hgvs_p": "p.Pro853Ala"
        }
      ],
      "clinvar_disease": "MYO16-related disorder",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "MYO16-related disorder",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}