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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 13-23334703-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=13&pos=23334703&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "13",
      "pos": 23334703,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000382292.9",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.9173T>G",
          "hgvs_p": "p.Leu3058Arg",
          "transcript": "NM_014363.6",
          "protein_id": "NP_055178.3",
          "transcript_support_level": null,
          "aa_start": 3058,
          "aa_end": null,
          "aa_length": 4579,
          "cds_start": 9173,
          "cds_end": null,
          "cds_length": 13740,
          "cdna_start": 9762,
          "cdna_end": null,
          "cdna_length": 15635,
          "mane_select": "ENST00000382292.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.9173T>G",
          "hgvs_p": "p.Leu3058Arg",
          "transcript": "ENST00000382292.9",
          "protein_id": "ENSP00000371729.3",
          "transcript_support_level": 5,
          "aa_start": 3058,
          "aa_end": null,
          "aa_length": 4579,
          "cds_start": 9173,
          "cds_end": null,
          "cds_length": 13740,
          "cdna_start": 9762,
          "cdna_end": null,
          "cdna_length": 15635,
          "mane_select": "NM_014363.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 10,
          "intron_rank_end": null,
          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.2432-5219T>G",
          "hgvs_p": null,
          "transcript": "ENST00000455470.6",
          "protein_id": "ENSP00000406565.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 832,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2499,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3674,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.9200T>G",
          "hgvs_p": "p.Leu3067Arg",
          "transcript": "NM_001437336.1",
          "protein_id": "NP_001424265.1",
          "transcript_support_level": null,
          "aa_start": 3067,
          "aa_end": null,
          "aa_length": 4588,
          "cds_start": 9200,
          "cds_end": null,
          "cds_length": 13767,
          "cdna_start": 9789,
          "cdna_end": null,
          "cdna_length": 15662,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.9200T>G",
          "hgvs_p": "p.Leu3067Arg",
          "transcript": "ENST00000682944.1",
          "protein_id": "ENSP00000507173.1",
          "transcript_support_level": null,
          "aa_start": 3067,
          "aa_end": null,
          "aa_length": 4588,
          "cds_start": 9200,
          "cds_end": null,
          "cds_length": 13767,
          "cdna_start": 9839,
          "cdna_end": null,
          "cdna_length": 15712,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.8732T>G",
          "hgvs_p": "p.Leu2911Arg",
          "transcript": "NM_001278055.2",
          "protein_id": "NP_001264984.1",
          "transcript_support_level": null,
          "aa_start": 2911,
          "aa_end": null,
          "aa_length": 4432,
          "cds_start": 8732,
          "cds_end": null,
          "cds_length": 13299,
          "cdna_start": 9222,
          "cdna_end": null,
          "cdna_length": 15095,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.6923T>G",
          "hgvs_p": "p.Leu2308Arg",
          "transcript": "ENST00000402364.1",
          "protein_id": "ENSP00000385844.1",
          "transcript_support_level": 2,
          "aa_start": 2308,
          "aa_end": null,
          "aa_length": 3829,
          "cds_start": 6923,
          "cds_end": null,
          "cds_length": 11490,
          "cdna_start": 9257,
          "cdna_end": null,
          "cdna_length": 15134,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.9224T>G",
          "hgvs_p": "p.Leu3075Arg",
          "transcript": "XM_047430254.1",
          "protein_id": "XP_047286210.1",
          "transcript_support_level": null,
          "aa_start": 3075,
          "aa_end": null,
          "aa_length": 4596,
          "cds_start": 9224,
          "cds_end": null,
          "cds_length": 13791,
          "cdna_start": 9261,
          "cdna_end": null,
          "cdna_length": 15134,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.9200T>G",
          "hgvs_p": "p.Leu3067Arg",
          "transcript": "XM_024449337.2",
          "protein_id": "XP_024305105.1",
          "transcript_support_level": null,
          "aa_start": 3067,
          "aa_end": null,
          "aa_length": 4588,
          "cds_start": 9200,
          "cds_end": null,
          "cds_length": 13767,
          "cdna_start": 10010,
          "cdna_end": null,
          "cdna_length": 15883,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.9197T>G",
          "hgvs_p": "p.Leu3066Arg",
          "transcript": "XM_047430255.1",
          "protein_id": "XP_047286211.1",
          "transcript_support_level": null,
          "aa_start": 3066,
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          "aa_length": 4587,
          "cds_start": 9197,
          "cds_end": null,
          "cds_length": 13764,
          "cdna_start": 9234,
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          "cdna_length": 15107,
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        },
        {
          "aa_ref": "L",
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
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          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.9191T>G",
          "hgvs_p": "p.Leu3064Arg",
          "transcript": "XM_011535039.3",
          "protein_id": "XP_011533341.1",
          "transcript_support_level": null,
          "aa_start": 3064,
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          "cds_start": 9191,
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          "cds_length": 13758,
          "cdna_start": 9337,
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        {
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          "exon_count": 10,
          "intron_rank": null,
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          "hgvs_c": "c.9173T>G",
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        {
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          ],
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          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.9164T>G",
          "hgvs_p": "p.Leu3055Arg",
          "transcript": "XM_017020539.2",
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        {
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          "intron_rank": null,
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          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.9053T>G",
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          "transcript": "XM_047430257.1",
          "protein_id": "XP_047286213.1",
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        {
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        {
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          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.9050T>G",
          "hgvs_p": "p.Leu3017Arg",
          "transcript": "XM_047430259.1",
          "protein_id": "XP_047286215.1",
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        {
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          "gene_symbol": "SACS",
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          "hgvs_c": "c.9044T>G",
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          "transcript": "XM_047430260.1",
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        {
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "SACS",
          "gene_hgnc_id": 10519,
          "hgvs_c": "c.9017T>G",
          "hgvs_p": "p.Leu3006Arg",
          "transcript": "XM_047430263.1",
          "protein_id": "XP_047286219.1",
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        },
        {
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          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
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      ],
      "gene_symbol": "SACS",
      "gene_hgnc_id": 10519,
      "dbsnp": "rs758654165",
      "frequency_reference_population": 0.000005577245,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 9,
      "gnomad_exomes_af": 0.00000478959,
      "gnomad_genomes_af": 0.0000131408,
      "gnomad_exomes_ac": 7,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8684358596801758,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.85,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9646,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.39,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 8.947,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate,BP6",
      "acmg_by_gene": [
        {
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          "benign_score": 1,
          "pathogenic_score": 4,
          "criteria": [
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            "PP3_Moderate",
            "BP6"
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          "verdict": "Uncertain_significance",
          "transcript": "ENST00000382292.9",
          "gene_symbol": "SACS",
          "hgnc_id": 10519,
          "effects": [
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          "inheritance_mode": "AR",
          "hgvs_c": "c.9173T>G",
          "hgvs_p": "p.Leu3058Arg"
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      ],
      "clinvar_disease": "Charlevoix-Saguenay spastic ataxia,Inborn genetic diseases,Spastic paraplegia,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:2 LB:1",
      "phenotype_combined": "not specified|Charlevoix-Saguenay spastic ataxia|Spastic paraplegia|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}