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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 13-28311732-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=13&pos=28311732&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "13",
      "pos": 28311732,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_002019.4",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLT1",
          "gene_hgnc_id": 3763,
          "hgvs_c": "c.3493G>A",
          "hgvs_p": "p.Asp1165Asn",
          "transcript": "NM_002019.4",
          "protein_id": "NP_002010.2",
          "transcript_support_level": null,
          "aa_start": 1165,
          "aa_end": null,
          "aa_length": 1338,
          "cds_start": 3493,
          "cds_end": null,
          "cds_length": 4017,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000282397.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002019.4"
        },
        {
          "aa_ref": "D",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLT1",
          "gene_hgnc_id": 3763,
          "hgvs_c": "c.3493G>A",
          "hgvs_p": "p.Asp1165Asn",
          "transcript": "ENST00000282397.9",
          "protein_id": "ENSP00000282397.4",
          "transcript_support_level": 1,
          "aa_start": 1165,
          "aa_end": null,
          "aa_length": 1338,
          "cds_start": 3493,
          "cds_end": null,
          "cds_length": 4017,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002019.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000282397.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLT1",
          "gene_hgnc_id": 3763,
          "hgvs_c": "n.1380G>A",
          "hgvs_p": null,
          "transcript": "ENST00000540678.2",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000540678.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLT1",
          "gene_hgnc_id": 3763,
          "hgvs_c": "n.718G>A",
          "hgvs_p": null,
          "transcript": "ENST00000543394.2",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000543394.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLT1",
          "gene_hgnc_id": 3763,
          "hgvs_c": "n.911G>A",
          "hgvs_p": null,
          "transcript": "ENST00000615611.4",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000615611.4"
        },
        {
          "aa_ref": "D",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLT1",
          "gene_hgnc_id": 3763,
          "hgvs_c": "c.3514G>A",
          "hgvs_p": "p.Asp1172Asn",
          "transcript": "ENST00000909998.1",
          "protein_id": "ENSP00000580057.1",
          "transcript_support_level": null,
          "aa_start": 1172,
          "aa_end": null,
          "aa_length": 1345,
          "cds_start": 3514,
          "cds_end": null,
          "cds_length": 4038,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909998.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLT1",
          "gene_hgnc_id": 3763,
          "hgvs_c": "c.3379G>A",
          "hgvs_p": "p.Asp1127Asn",
          "transcript": "ENST00000910000.1",
          "protein_id": "ENSP00000580059.1",
          "transcript_support_level": null,
          "aa_start": 1127,
          "aa_end": null,
          "aa_length": 1300,
          "cds_start": 3379,
          "cds_end": null,
          "cds_length": 3903,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910000.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLT1",
          "gene_hgnc_id": 3763,
          "hgvs_c": "c.3169G>A",
          "hgvs_p": "p.Asp1057Asn",
          "transcript": "ENST00000909997.1",
          "protein_id": "ENSP00000580056.1",
          "transcript_support_level": null,
          "aa_start": 1057,
          "aa_end": null,
          "aa_length": 1230,
          "cds_start": 3169,
          "cds_end": null,
          "cds_length": 3693,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909997.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLT1",
          "gene_hgnc_id": 3763,
          "hgvs_c": "c.1588G>A",
          "hgvs_p": "p.Asp530Asn",
          "transcript": "ENST00000909999.1",
          "protein_id": "ENSP00000580058.1",
          "transcript_support_level": null,
          "aa_start": 530,
          "aa_end": null,
          "aa_length": 703,
          "cds_start": 1588,
          "cds_end": null,
          "cds_length": 2112,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909999.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLT1",
          "gene_hgnc_id": 3763,
          "hgvs_c": "n.1318G>A",
          "hgvs_p": null,
          "transcript": "ENST00000706527.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000706527.1"
        }
      ],
      "gene_symbol": "FLT1",
      "gene_hgnc_id": 3763,
      "dbsnp": null,
      "frequency_reference_population": 6.8411805e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84118e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3411249816417694,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.9520000219345093,
      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.396,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1025,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.23,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.081,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.25,
      "spliceai_max_prediction": "Uncertain_significance",
      "dbscsnv_ada_score": 0.998844486069746,
      "dbscsnv_ada_prediction": "Pathogenic",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_002019.4",
          "gene_symbol": "FLT1",
          "hgnc_id": 3763,
          "effects": [
            "missense_variant",
            "splice_region_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.3493G>A",
          "hgvs_p": "p.Asp1165Asn"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}