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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 13-49541798-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=13&pos=49541798&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "13",
      "pos": 49541798,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000378302.7",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "c.1202C>T",
          "hgvs_p": "p.Ser401Leu",
          "transcript": "NM_018191.4",
          "protein_id": "NP_060661.3",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 531,
          "cds_start": 1202,
          "cds_end": null,
          "cds_length": 1596,
          "cdna_start": 1438,
          "cdna_end": null,
          "cdna_length": 4008,
          "mane_select": "ENST00000378302.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "c.1202C>T",
          "hgvs_p": "p.Ser401Leu",
          "transcript": "ENST00000378302.7",
          "protein_id": "ENSP00000367552.2",
          "transcript_support_level": 1,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 531,
          "cds_start": 1202,
          "cds_end": null,
          "cds_length": 1596,
          "cdna_start": 1438,
          "cdna_end": null,
          "cdna_length": 4008,
          "mane_select": "NM_018191.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "c.1202C>T",
          "hgvs_p": "p.Ser401Leu",
          "transcript": "NM_001352500.2",
          "protein_id": "NP_001339429.1",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 531,
          "cds_start": 1202,
          "cds_end": null,
          "cds_length": 1596,
          "cdna_start": 1441,
          "cdna_end": null,
          "cdna_length": 4011,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "c.1202C>T",
          "hgvs_p": "p.Ser401Leu",
          "transcript": "NM_001352501.2",
          "protein_id": "NP_001339430.1",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 531,
          "cds_start": 1202,
          "cds_end": null,
          "cds_length": 1596,
          "cdna_start": 1358,
          "cdna_end": null,
          "cdna_length": 3928,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "c.1202C>T",
          "hgvs_p": "p.Ser401Leu",
          "transcript": "NM_001352502.2",
          "protein_id": "NP_001339431.1",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 531,
          "cds_start": 1202,
          "cds_end": null,
          "cds_length": 1596,
          "cdna_start": 1361,
          "cdna_end": null,
          "cdna_length": 3931,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "c.1202C>T",
          "hgvs_p": "p.Ser401Leu",
          "transcript": "NM_001352503.2",
          "protein_id": "NP_001339432.1",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 531,
          "cds_start": 1202,
          "cds_end": null,
          "cds_length": 1596,
          "cdna_start": 1434,
          "cdna_end": null,
          "cdna_length": 4004,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "c.1202C>T",
          "hgvs_p": "p.Ser401Leu",
          "transcript": "ENST00000258646.3",
          "protein_id": "ENSP00000258646.3",
          "transcript_support_level": 2,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 531,
          "cds_start": 1202,
          "cds_end": null,
          "cds_length": 1596,
          "cdna_start": 1246,
          "cdna_end": null,
          "cdna_length": 3816,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "c.1202C>T",
          "hgvs_p": "p.Ser401Leu",
          "transcript": "NM_001352504.2",
          "protein_id": "NP_001339433.1",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 487,
          "cds_start": 1202,
          "cds_end": null,
          "cds_length": 1464,
          "cdna_start": 1438,
          "cdna_end": null,
          "cdna_length": 2159,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "c.623C>T",
          "hgvs_p": "p.Ser208Leu",
          "transcript": "NM_001352506.2",
          "protein_id": "NP_001339435.1",
          "transcript_support_level": null,
          "aa_start": 208,
          "aa_end": null,
          "aa_length": 338,
          "cds_start": 623,
          "cds_end": null,
          "cds_length": 1017,
          "cdna_start": 1468,
          "cdna_end": null,
          "cdna_length": 4038,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "c.1202C>T",
          "hgvs_p": "p.Ser401Leu",
          "transcript": "XM_011535134.2",
          "protein_id": "XP_011533436.1",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 531,
          "cds_start": 1202,
          "cds_end": null,
          "cds_length": 1596,
          "cdna_start": 1437,
          "cdna_end": null,
          "cdna_length": 4007,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "c.815C>T",
          "hgvs_p": "p.Ser272Leu",
          "transcript": "XM_011535135.3",
          "protein_id": "XP_011533437.1",
          "transcript_support_level": null,
          "aa_start": 272,
          "aa_end": null,
          "aa_length": 402,
          "cds_start": 815,
          "cds_end": null,
          "cds_length": 1209,
          "cdna_start": 974,
          "cdna_end": null,
          "cdna_length": 3544,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "n.1596C>T",
          "hgvs_p": null,
          "transcript": "NR_148015.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4166,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RCBTB1",
          "gene_hgnc_id": 18243,
          "hgvs_c": "n.1552C>T",
          "hgvs_p": null,
          "transcript": "NR_148016.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4122,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "RCBTB1",
      "gene_hgnc_id": 18243,
      "dbsnp": "rs556664001",
      "frequency_reference_population": 0.000019834679,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 32,
      "gnomad_exomes_af": 0.0000184779,
      "gnomad_genomes_af": 0.0000328666,
      "gnomad_exomes_ac": 27,
      "gnomad_genomes_ac": 5,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.740953803062439,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.448,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.2391,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.19,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.221,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 0,
          "pathogenic_score": 0,
          "criteria": [],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000378302.7",
          "gene_symbol": "RCBTB1",
          "hgnc_id": 18243,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.1202C>T",
          "hgvs_p": "p.Ser401Leu"
        }
      ],
      "clinvar_disease": "Retinitis pigmentosa,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Retinitis pigmentosa|not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}