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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 13-60015973-T-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=13&pos=60015973&ref=T&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "13",
      "pos": 60015973,
      "ref": "T",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001042517.2",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.711A>T",
          "hgvs_p": "p.Lys237Asn",
          "transcript": "NM_001042517.2",
          "protein_id": "NP_001035982.1",
          "transcript_support_level": null,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 1193,
          "cds_start": 711,
          "cds_end": null,
          "cds_length": 3582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000400324.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001042517.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.711A>T",
          "hgvs_p": "p.Lys237Asn",
          "transcript": "ENST00000400324.9",
          "protein_id": "ENSP00000383178.3",
          "transcript_support_level": 1,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 1193,
          "cds_start": 711,
          "cds_end": null,
          "cds_length": 3582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001042517.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000400324.9"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.678A>T",
          "hgvs_p": "p.Lys226Asn",
          "transcript": "ENST00000377908.6",
          "protein_id": "ENSP00000367141.2",
          "transcript_support_level": 1,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 1182,
          "cds_start": 678,
          "cds_end": null,
          "cds_length": 3549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000377908.6"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.573A>T",
          "hgvs_p": "p.Lys191Asn",
          "transcript": "ENST00000400320.5",
          "protein_id": "ENSP00000383174.1",
          "transcript_support_level": 1,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 1147,
          "cds_start": 573,
          "cds_end": null,
          "cds_length": 3444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000400320.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.501A>T",
          "hgvs_p": "p.Lys167Asn",
          "transcript": "ENST00000400319.5",
          "protein_id": "ENSP00000383173.1",
          "transcript_support_level": 1,
          "aa_start": 167,
          "aa_end": null,
          "aa_length": 1123,
          "cds_start": 501,
          "cds_end": null,
          "cds_length": 3372,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000400319.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.711A>T",
          "hgvs_p": "p.Lys237Asn",
          "transcript": "ENST00000267215.8",
          "protein_id": "ENSP00000267215.4",
          "transcript_support_level": 1,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 1112,
          "cds_start": 711,
          "cds_end": null,
          "cds_length": 3339,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000267215.8"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.678A>T",
          "hgvs_p": "p.Lys226Asn",
          "transcript": "NM_001258366.2",
          "protein_id": "NP_001245295.1",
          "transcript_support_level": null,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 1182,
          "cds_start": 678,
          "cds_end": null,
          "cds_length": 3549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001258366.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.573A>T",
          "hgvs_p": "p.Lys191Asn",
          "transcript": "NM_001258367.2",
          "protein_id": "NP_001245296.1",
          "transcript_support_level": null,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 1147,
          "cds_start": 573,
          "cds_end": null,
          "cds_length": 3444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001258367.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.501A>T",
          "hgvs_p": "p.Lys167Asn",
          "transcript": "NM_001258368.2",
          "protein_id": "NP_001245297.1",
          "transcript_support_level": null,
          "aa_start": 167,
          "aa_end": null,
          "aa_length": 1123,
          "cds_start": 501,
          "cds_end": null,
          "cds_length": 3372,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001258368.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.711A>T",
          "hgvs_p": "p.Lys237Asn",
          "transcript": "NM_001258369.2",
          "protein_id": "NP_001245298.1",
          "transcript_support_level": null,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 1112,
          "cds_start": 711,
          "cds_end": null,
          "cds_length": 3339,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001258369.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.711A>T",
          "hgvs_p": "p.Lys237Asn",
          "transcript": "XM_024449422.1",
          "protein_id": "XP_024305190.1",
          "transcript_support_level": null,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 1143,
          "cds_start": 711,
          "cds_end": null,
          "cds_length": 3432,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024449422.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.711A>T",
          "hgvs_p": "p.Lys237Asn",
          "transcript": "XM_011535258.3",
          "protein_id": "XP_011533560.1",
          "transcript_support_level": null,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 1136,
          "cds_start": 711,
          "cds_end": null,
          "cds_length": 3411,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535258.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.711A>T",
          "hgvs_p": "p.Lys237Asn",
          "transcript": "XM_011535263.3",
          "protein_id": "XP_011533565.1",
          "transcript_support_level": null,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 737,
          "cds_start": 711,
          "cds_end": null,
          "cds_length": 2214,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535263.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.678A>T",
          "hgvs_p": "p.Lys226Asn",
          "transcript": "XM_047430701.1",
          "protein_id": "XP_047286657.1",
          "transcript_support_level": null,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 726,
          "cds_start": 678,
          "cds_end": null,
          "cds_length": 2181,
          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047430701.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.711A>T",
          "hgvs_p": "p.Lys237Asn",
          "transcript": "XM_011535265.3",
          "protein_id": "XP_011533567.1",
          "transcript_support_level": null,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 713,
          "cds_start": 711,
          "cds_end": null,
          "cds_length": 2142,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535265.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "c.711A>T",
          "hgvs_p": "p.Lys237Asn",
          "transcript": "XM_017020789.1",
          "protein_id": "XP_016876278.1",
          "transcript_support_level": null,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 711,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017020789.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "n.873A>T",
          "hgvs_p": null,
          "transcript": "XR_001749694.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_001749694.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "n.873A>T",
          "hgvs_p": null,
          "transcript": "XR_002957477.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_002957477.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "n.873A>T",
          "hgvs_p": null,
          "transcript": "XR_002957478.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_002957478.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DIAPH3",
          "gene_hgnc_id": 15480,
          "hgvs_c": "n.873A>T",
          "hgvs_p": null,
          "transcript": "XR_002957480.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_002957480.1"
        },
        {
          "aa_ref": null,
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      "computational_score_selected": 0.003138422966003418,
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      "computational_source_selected": "MetaRNN",
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      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Benign",
      "alphamissense_score": 0.1006,
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      "bayesdelnoaf_score": -0.4,
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      "phylop100way_score": 0.06,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
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      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS1,BS2",
      "acmg_by_gene": [
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          "pathogenic_score": 0,
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            "BS1",
            "BS2"
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          "verdict": "Benign",
          "transcript": "NM_001042517.2",
          "gene_symbol": "DIAPH3",
          "hgnc_id": 15480,
          "effects": [
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          "inheritance_mode": "AD,Unknown",
          "hgvs_c": "c.711A>T",
          "hgvs_p": "p.Lys237Asn"
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        {
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            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000435636.6",
          "gene_symbol": "DIAPH3-AS1",
          "hgnc_id": 39915,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.150+3115T>A",
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      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:2",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
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  ],
  "message": null
}