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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-20693772-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=20693772&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 20693772,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000397990.5",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANG",
          "gene_hgnc_id": 483,
          "hgvs_c": "c.208A>G",
          "hgvs_p": "p.Ile70Val",
          "transcript": "NM_001097577.3",
          "protein_id": "NP_001091046.1",
          "transcript_support_level": null,
          "aa_start": 70,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": 208,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": 337,
          "cdna_end": null,
          "cdna_length": 748,
          "mane_select": "ENST00000397990.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANG",
          "gene_hgnc_id": 483,
          "hgvs_c": "c.208A>G",
          "hgvs_p": "p.Ile70Val",
          "transcript": "ENST00000397990.5",
          "protein_id": "ENSP00000381077.4",
          "transcript_support_level": 1,
          "aa_start": 70,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": 208,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": 337,
          "cdna_end": null,
          "cdna_length": 748,
          "mane_select": "NM_001097577.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANG",
          "gene_hgnc_id": 483,
          "hgvs_c": "c.208A>G",
          "hgvs_p": "p.Ile70Val",
          "transcript": "ENST00000336811.10",
          "protein_id": "ENSP00000336762.6",
          "transcript_support_level": 1,
          "aa_start": 70,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": 208,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": 808,
          "cdna_end": null,
          "cdna_length": 1222,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RNASE4",
          "gene_hgnc_id": 10047,
          "hgvs_c": "c.-17-5583A>G",
          "hgvs_p": null,
          "transcript": "NM_002937.5",
          "protein_id": "NP_002928.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2061,
          "mane_select": "ENST00000555835.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RNASE4",
          "gene_hgnc_id": 10047,
          "hgvs_c": "c.-17-5583A>G",
          "hgvs_p": null,
          "transcript": "ENST00000555835.3",
          "protein_id": "ENSP00000452245.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2061,
          "mane_select": "NM_002937.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000259171",
          "gene_hgnc_id": null,
          "hgvs_c": "c.86+122A>G",
          "hgvs_p": null,
          "transcript": "ENST00000553909.1",
          "protein_id": "ENSP00000477037.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 31,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 96,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1498,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANG",
          "gene_hgnc_id": 483,
          "hgvs_c": "c.208A>G",
          "hgvs_p": "p.Ile70Val",
          "transcript": "NM_001145.4",
          "protein_id": "NP_001136.1",
          "transcript_support_level": null,
          "aa_start": 70,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": 208,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": 808,
          "cdna_end": null,
          "cdna_length": 1222,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANG",
          "gene_hgnc_id": 483,
          "hgvs_c": "c.208A>G",
          "hgvs_p": "p.Ile70Val",
          "transcript": "NM_001385271.1",
          "protein_id": "NP_001372200.1",
          "transcript_support_level": null,
          "aa_start": 70,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": 208,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": 549,
          "cdna_end": null,
          "cdna_length": 960,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANG",
          "gene_hgnc_id": 483,
          "hgvs_c": "c.208A>G",
          "hgvs_p": "p.Ile70Val",
          "transcript": "NM_001385272.1",
          "protein_id": "NP_001372201.1",
          "transcript_support_level": null,
          "aa_start": 70,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": 208,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": 458,
          "cdna_end": null,
          "cdna_length": 869,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANG",
          "gene_hgnc_id": 483,
          "hgvs_c": "c.208A>G",
          "hgvs_p": "p.Ile70Val",
          "transcript": "NM_001385273.1",
          "protein_id": "NP_001372202.1",
          "transcript_support_level": null,
          "aa_start": 70,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": 208,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": 363,
          "cdna_end": null,
          "cdna_length": 774,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANG",
          "gene_hgnc_id": 483,
          "hgvs_c": "c.208A>G",
          "hgvs_p": "p.Ile70Val",
          "transcript": "NM_001385274.1",
          "protein_id": "NP_001372203.1",
          "transcript_support_level": null,
          "aa_start": 70,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": 208,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": 318,
          "cdna_end": null,
          "cdna_length": 729,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "RNASE4",
          "gene_hgnc_id": 10047,
          "hgvs_c": "c.-18+122A>G",
          "hgvs_p": null,
          "transcript": "NM_001282192.2",
          "protein_id": "NP_001269121.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2165,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RNASE4",
          "gene_hgnc_id": 10047,
          "hgvs_c": "c.-17-5583A>G",
          "hgvs_p": null,
          "transcript": "NM_001282193.2",
          "protein_id": "NP_001269122.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": null,
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          "cdna_length": 1999,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RNASE4",
          "gene_hgnc_id": 10047,
          "hgvs_c": "c.-18+4898A>G",
          "hgvs_p": null,
          "transcript": "NM_194431.3",
          "protein_id": "NP_919412.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1973,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RNASE4",
          "gene_hgnc_id": 10047,
          "hgvs_c": "c.-17-5583A>G",
          "hgvs_p": null,
          "transcript": "ENST00000397995.2",
          "protein_id": "ENSP00000381081.2",
          "transcript_support_level": 2,
          "aa_start": null,
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          "aa_length": 147,
          "cds_start": -4,
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          "cds_length": 444,
          "cdna_start": null,
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          "cdna_length": 853,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RNASE4",
          "gene_hgnc_id": 10047,
          "hgvs_c": "c.-18+4898A>G",
          "hgvs_p": null,
          "transcript": "ENST00000555597.1",
          "protein_id": "ENSP00000451624.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1341,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ANG",
          "gene_hgnc_id": 483,
          "hgvs_c": "n.146-5094A>G",
          "hgvs_p": null,
          "transcript": "ENST00000554073.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 251,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "EGILA",
          "gene_hgnc_id": 54482,
          "hgvs_c": "n.646-23T>C",
          "hgvs_p": null,
          "transcript": "ENST00000554286.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 915,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000303727",
          "gene_hgnc_id": null,
          "hgvs_c": "n.153-6978T>C",
          "hgvs_p": null,
          "transcript": "ENST00000796740.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 339,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "EGILA",
          "gene_hgnc_id": 54482,
          "hgvs_c": "n.467-23T>C",
          "hgvs_p": null,
          "transcript": "NR_174964.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 736,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "ANG",
      "gene_hgnc_id": 483,
      "dbsnp": "rs121909541",
      "frequency_reference_population": 0.0006907309,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1115,
      "gnomad_exomes_af": 0.000688148,
      "gnomad_genomes_af": 0.000715514,
      "gnomad_exomes_ac": 1006,
      "gnomad_genomes_ac": 109,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.12110468745231628,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.595,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1402,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.05,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 0.228,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM1,BP4_Moderate,BP6,BS2",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 7,
          "pathogenic_score": 2,
          "criteria": [
            "PM1",
            "BP4_Moderate",
            "BP6",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000397990.5",
          "gene_symbol": "ANG",
          "hgnc_id": 483,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.208A>G",
          "hgvs_p": "p.Ile70Val"
        },
        {
          "score": -3,
          "benign_score": 3,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000555835.3",
          "gene_symbol": "RNASE4",
          "hgnc_id": 10047,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-17-5583A>G",
          "hgvs_p": null
        },
        {
          "score": -3,
          "benign_score": 3,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000553909.1",
          "gene_symbol": "ENSG00000259171",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.86+122A>G",
          "hgvs_p": null
        },
        {
          "score": -3,
          "benign_score": 3,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "NR_174964.1",
          "gene_symbol": "EGILA",
          "hgnc_id": 54482,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.467-23T>C",
          "hgvs_p": null
        },
        {
          "score": -3,
          "benign_score": 3,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000796740.1",
          "gene_symbol": "ENSG00000303727",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.153-6978T>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "ANG-related disorder,Amyotrophic lateral sclerosis type 9,not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:2 LB:4",
      "phenotype_combined": "Amyotrophic lateral sclerosis type 9|not provided|not specified|ANG-related disorder",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}