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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-31293573-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=31293573&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 31293573,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_015473.4",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.5873C>T",
          "hgvs_p": "p.Ser1958Phe",
          "transcript": "NM_015473.4",
          "protein_id": "NP_056288.2",
          "transcript_support_level": null,
          "aa_start": 1958,
          "aa_end": null,
          "aa_length": 2046,
          "cds_start": 5873,
          "cds_end": null,
          "cds_length": 6141,
          "cdna_start": 6026,
          "cdna_end": null,
          "cdna_length": 7811,
          "mane_select": "ENST00000543095.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015473.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.5873C>T",
          "hgvs_p": "p.Ser1958Phe",
          "transcript": "ENST00000543095.7",
          "protein_id": "ENSP00000437968.2",
          "transcript_support_level": 5,
          "aa_start": 1958,
          "aa_end": null,
          "aa_length": 2046,
          "cds_start": 5873,
          "cds_end": null,
          "cds_length": 6141,
          "cdna_start": 6026,
          "cdna_end": null,
          "cdna_length": 7811,
          "mane_select": "NM_015473.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000543095.7"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 37,
          "exon_rank_end": null,
          "exon_count": 37,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.5939C>T",
          "hgvs_p": "p.Ser1980Phe",
          "transcript": "ENST00000925437.1",
          "protein_id": "ENSP00000595496.1",
          "transcript_support_level": null,
          "aa_start": 1980,
          "aa_end": null,
          "aa_length": 2068,
          "cds_start": 5939,
          "cds_end": null,
          "cds_length": 6207,
          "cdna_start": 6071,
          "cdna_end": null,
          "cdna_length": 7851,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925437.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.5870C>T",
          "hgvs_p": "p.Ser1957Phe",
          "transcript": "ENST00000892404.1",
          "protein_id": "ENSP00000562463.1",
          "transcript_support_level": null,
          "aa_start": 1957,
          "aa_end": null,
          "aa_length": 2045,
          "cds_start": 5870,
          "cds_end": null,
          "cds_length": 6138,
          "cdna_start": 6080,
          "cdna_end": null,
          "cdna_length": 9017,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892404.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.5804C>T",
          "hgvs_p": "p.Ser1935Phe",
          "transcript": "ENST00000925433.1",
          "protein_id": "ENSP00000595492.1",
          "transcript_support_level": null,
          "aa_start": 1935,
          "aa_end": null,
          "aa_length": 2023,
          "cds_start": 5804,
          "cds_end": null,
          "cds_length": 6072,
          "cdna_start": 5955,
          "cdna_end": null,
          "cdna_length": 7740,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925433.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 35,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.5705C>T",
          "hgvs_p": "p.Ser1902Phe",
          "transcript": "ENST00000925434.1",
          "protein_id": "ENSP00000595493.1",
          "transcript_support_level": null,
          "aa_start": 1902,
          "aa_end": null,
          "aa_length": 1990,
          "cds_start": 5705,
          "cds_end": null,
          "cds_length": 5973,
          "cdna_start": 5856,
          "cdna_end": null,
          "cdna_length": 7641,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925434.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 35,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.5693C>T",
          "hgvs_p": "p.Ser1898Phe",
          "transcript": "ENST00000925435.1",
          "protein_id": "ENSP00000595494.1",
          "transcript_support_level": null,
          "aa_start": 1898,
          "aa_end": null,
          "aa_length": 1986,
          "cds_start": 5693,
          "cds_end": null,
          "cds_length": 5961,
          "cdna_start": 5844,
          "cdna_end": null,
          "cdna_length": 7629,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925435.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 35,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.5672C>T",
          "hgvs_p": "p.Ser1891Phe",
          "transcript": "ENST00000892408.1",
          "protein_id": "ENSP00000562467.1",
          "transcript_support_level": null,
          "aa_start": 1891,
          "aa_end": null,
          "aa_length": 1979,
          "cds_start": 5672,
          "cds_end": null,
          "cds_length": 5940,
          "cdna_start": 5825,
          "cdna_end": null,
          "cdna_length": 6796,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892408.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 35,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.5633C>T",
          "hgvs_p": "p.Ser1878Phe",
          "transcript": "ENST00000892406.1",
          "protein_id": "ENSP00000562465.1",
          "transcript_support_level": null,
          "aa_start": 1878,
          "aa_end": null,
          "aa_length": 1966,
          "cds_start": 5633,
          "cds_end": null,
          "cds_length": 5901,
          "cdna_start": 5827,
          "cdna_end": null,
          "cdna_length": 6816,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892406.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 35,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.5624C>T",
          "hgvs_p": "p.Ser1875Phe",
          "transcript": "ENST00000892405.1",
          "protein_id": "ENSP00000562464.1",
          "transcript_support_level": null,
          "aa_start": 1875,
          "aa_end": null,
          "aa_length": 1963,
          "cds_start": 5624,
          "cds_end": null,
          "cds_length": 5892,
          "cdna_start": 5777,
          "cdna_end": null,
          "cdna_length": 7559,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892405.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 35,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.5600C>T",
          "hgvs_p": "p.Ser1867Phe",
          "transcript": "ENST00000925436.1",
          "protein_id": "ENSP00000595495.1",
          "transcript_support_level": null,
          "aa_start": 1867,
          "aa_end": null,
          "aa_length": 1955,
          "cds_start": 5600,
          "cds_end": null,
          "cds_length": 5868,
          "cdna_start": 5752,
          "cdna_end": null,
          "cdna_length": 7536,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925436.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 34,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.5375C>T",
          "hgvs_p": "p.Ser1792Phe",
          "transcript": "ENST00000892407.1",
          "protein_id": "ENSP00000562466.1",
          "transcript_support_level": null,
          "aa_start": 1792,
          "aa_end": null,
          "aa_length": 1880,
          "cds_start": 5375,
          "cds_end": null,
          "cds_length": 5643,
          "cdna_start": 5528,
          "cdna_end": null,
          "cdna_length": 6517,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892407.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "c.4529C>T",
          "hgvs_p": "p.Ser1510Phe",
          "transcript": "ENST00000538864.6",
          "protein_id": "ENSP00000439979.2",
          "transcript_support_level": 5,
          "aa_start": 1510,
          "aa_end": null,
          "aa_length": 1598,
          "cds_start": 4529,
          "cds_end": null,
          "cds_length": 4797,
          "cdna_start": 4531,
          "cdna_end": null,
          "cdna_length": 5237,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000538864.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HEATR5A",
          "gene_hgnc_id": 20276,
          "hgvs_c": "n.2066C>T",
          "hgvs_p": null,
          "transcript": "ENST00000551414.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3037,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000551414.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000257831",
          "gene_hgnc_id": null,
          "hgvs_c": "n.401-1859G>A",
          "hgvs_p": null,
          "transcript": "ENST00000551799.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 975,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000551799.1"
        }
      ],
      "gene_symbol": "HEATR5A",
      "gene_hgnc_id": 20276,
      "dbsnp": "rs199961649",
      "frequency_reference_population": 0.000069424554,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 112,
      "gnomad_exomes_af": 0.0000561223,
      "gnomad_genomes_af": 0.000197151,
      "gnomad_exomes_ac": 82,
      "gnomad_genomes_ac": 30,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7469905614852905,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.834,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.3268,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": 0.29,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.932,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP3",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 0,
          "pathogenic_score": 1,
          "criteria": [
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_015473.4",
          "gene_symbol": "HEATR5A",
          "hgnc_id": 20276,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.5873C>T",
          "hgvs_p": "p.Ser1958Phe"
        },
        {
          "score": 1,
          "benign_score": 0,
          "pathogenic_score": 1,
          "criteria": [
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000551799.1",
          "gene_symbol": "ENSG00000257831",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.401-1859G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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