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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-35095103-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=35095103&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 35095103,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_017917.4",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.920C>G",
          "hgvs_p": "p.Thr307Ser",
          "transcript": "NM_017917.4",
          "protein_id": "NP_060387.2",
          "transcript_support_level": null,
          "aa_start": 307,
          "aa_end": null,
          "aa_length": 453,
          "cds_start": 920,
          "cds_end": null,
          "cds_length": 1362,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000261475.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017917.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.920C>G",
          "hgvs_p": "p.Thr307Ser",
          "transcript": "ENST00000261475.10",
          "protein_id": "ENSP00000261475.5",
          "transcript_support_level": 1,
          "aa_start": 307,
          "aa_end": null,
          "aa_length": 453,
          "cds_start": 920,
          "cds_end": null,
          "cds_length": 1362,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_017917.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000261475.10"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "n.*723C>G",
          "hgvs_p": null,
          "transcript": "ENST00000557217.5",
          "protein_id": "ENSP00000452436.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000557217.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "n.*723C>G",
          "hgvs_p": null,
          "transcript": "ENST00000557217.5",
          "protein_id": "ENSP00000452436.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000557217.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "n.*641+1455C>G",
          "hgvs_p": null,
          "transcript": "ENST00000553273.5",
          "protein_id": "ENSP00000451075.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000553273.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.941C>G",
          "hgvs_p": "p.Thr314Ser",
          "transcript": "ENST00000892731.1",
          "protein_id": "ENSP00000562790.1",
          "transcript_support_level": null,
          "aa_start": 314,
          "aa_end": null,
          "aa_length": 460,
          "cds_start": 941,
          "cds_end": null,
          "cds_length": 1383,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892731.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.890C>G",
          "hgvs_p": "p.Thr297Ser",
          "transcript": "ENST00000928308.1",
          "protein_id": "ENSP00000598367.1",
          "transcript_support_level": null,
          "aa_start": 297,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 890,
          "cds_end": null,
          "cds_length": 1332,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928308.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.638C>G",
          "hgvs_p": "p.Thr213Ser",
          "transcript": "ENST00000928307.1",
          "protein_id": "ENSP00000598366.1",
          "transcript_support_level": null,
          "aa_start": 213,
          "aa_end": null,
          "aa_length": 359,
          "cds_start": 638,
          "cds_end": null,
          "cds_length": 1080,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928307.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.638C>G",
          "hgvs_p": "p.Thr213Ser",
          "transcript": "ENST00000955603.1",
          "protein_id": "ENSP00000625662.1",
          "transcript_support_level": null,
          "aa_start": 213,
          "aa_end": null,
          "aa_length": 359,
          "cds_start": 638,
          "cds_end": null,
          "cds_length": 1080,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955603.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.590C>G",
          "hgvs_p": "p.Thr197Ser",
          "transcript": "NM_001305155.2",
          "protein_id": "NP_001292084.1",
          "transcript_support_level": null,
          "aa_start": 197,
          "aa_end": null,
          "aa_length": 343,
          "cds_start": 590,
          "cds_end": null,
          "cds_length": 1032,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001305155.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.590C>G",
          "hgvs_p": "p.Thr197Ser",
          "transcript": "NM_001305156.2",
          "protein_id": "NP_001292085.1",
          "transcript_support_level": null,
          "aa_start": 197,
          "aa_end": null,
          "aa_length": 343,
          "cds_start": 590,
          "cds_end": null,
          "cds_length": 1032,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001305156.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.506C>G",
          "hgvs_p": "p.Thr169Ser",
          "transcript": "ENST00000928309.1",
          "protein_id": "ENSP00000598368.1",
          "transcript_support_level": null,
          "aa_start": 169,
          "aa_end": null,
          "aa_length": 315,
          "cds_start": 506,
          "cds_end": null,
          "cds_length": 948,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928309.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.83C>G",
          "hgvs_p": "p.Thr28Ser",
          "transcript": "ENST00000555219.1",
          "protein_id": "ENSP00000452173.1",
          "transcript_support_level": 3,
          "aa_start": 28,
          "aa_end": null,
          "aa_length": 128,
          "cds_start": 83,
          "cds_end": null,
          "cds_length": 387,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000555219.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.920C>G",
          "hgvs_p": "p.Thr307Ser",
          "transcript": "XM_005267782.5",
          "protein_id": "XP_005267839.1",
          "transcript_support_level": null,
          "aa_start": 307,
          "aa_end": null,
          "aa_length": 453,
          "cds_start": 920,
          "cds_end": null,
          "cds_length": 1362,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005267782.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.827C>G",
          "hgvs_p": "p.Thr276Ser",
          "transcript": "XM_024449638.2",
          "protein_id": "XP_024305406.1",
          "transcript_support_level": null,
          "aa_start": 276,
          "aa_end": null,
          "aa_length": 422,
          "cds_start": 827,
          "cds_end": null,
          "cds_length": 1269,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024449638.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.764C>G",
          "hgvs_p": "p.Thr255Ser",
          "transcript": "XM_024449639.2",
          "protein_id": "XP_024305407.1",
          "transcript_support_level": null,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 401,
          "cds_start": 764,
          "cds_end": null,
          "cds_length": 1206,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024449639.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.187-3896C>G",
          "hgvs_p": null,
          "transcript": "ENST00000928310.1",
          "protein_id": "ENSP00000598369.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 190,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 573,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928310.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 10,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "c.838+1455C>G",
          "hgvs_p": null,
          "transcript": "XM_047431517.1",
          "protein_id": "XP_047287473.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 281,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 846,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047431517.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "n.*723C>G",
          "hgvs_p": null,
          "transcript": "ENST00000554222.5",
          "protein_id": "ENSP00000451416.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000554222.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP2R3C",
          "gene_hgnc_id": 17485,
          "hgvs_c": "n.*623C>G",
          "hgvs_p": null,
          "transcript": "ENST00000554899.5",
          "protein_id": "ENSP00000452263.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
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        {
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      ],
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      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.000002746,
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      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.925283670425415,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.431,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.5787,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.05,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 9.855,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
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            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_017917.4",
          "gene_symbol": "PPP2R3C",
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          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
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          "hgvs_p": "p.Thr307Ser"
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        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000555260.1",
          "gene_symbol": "FAM177A1",
          "hgnc_id": 19829,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
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        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NR_110415.1",
          "gene_symbol": "LOC101927178",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.480-4549G>C",
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        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}