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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-45196400-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=45196400&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 45196400,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000267430.10",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.5569G>A",
          "hgvs_p": "p.Val1857Met",
          "transcript": "NM_020937.4",
          "protein_id": "NP_065988.1",
          "transcript_support_level": null,
          "aa_start": 1857,
          "aa_end": null,
          "aa_length": 2048,
          "cds_start": 5569,
          "cds_end": null,
          "cds_length": 6147,
          "cdna_start": 5671,
          "cdna_end": null,
          "cdna_length": 7131,
          "mane_select": "ENST00000267430.10",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.5569G>A",
          "hgvs_p": "p.Val1857Met",
          "transcript": "ENST00000267430.10",
          "protein_id": "ENSP00000267430.5",
          "transcript_support_level": 1,
          "aa_start": 1857,
          "aa_end": null,
          "aa_length": 2048,
          "cds_start": 5569,
          "cds_end": null,
          "cds_length": 6147,
          "cdna_start": 5671,
          "cdna_end": null,
          "cdna_length": 7131,
          "mane_select": "NM_020937.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.5491G>A",
          "hgvs_p": "p.Val1831Met",
          "transcript": "ENST00000542564.6",
          "protein_id": "ENSP00000442493.2",
          "transcript_support_level": 1,
          "aa_start": 1831,
          "aa_end": null,
          "aa_length": 2022,
          "cds_start": 5491,
          "cds_end": null,
          "cds_length": 6069,
          "cdna_start": 5569,
          "cdna_end": null,
          "cdna_length": 6167,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.5362G>A",
          "hgvs_p": "p.Val1788Met",
          "transcript": "ENST00000556250.6",
          "protein_id": "ENSP00000452033.2",
          "transcript_support_level": 1,
          "aa_start": 1788,
          "aa_end": null,
          "aa_length": 1979,
          "cds_start": 5362,
          "cds_end": null,
          "cds_length": 5940,
          "cdna_start": 5464,
          "cdna_end": null,
          "cdna_length": 6924,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.5491G>A",
          "hgvs_p": "p.Val1831Met",
          "transcript": "NM_001308133.2",
          "protein_id": "NP_001295062.1",
          "transcript_support_level": null,
          "aa_start": 1831,
          "aa_end": null,
          "aa_length": 2022,
          "cds_start": 5491,
          "cds_end": null,
          "cds_length": 6069,
          "cdna_start": 5593,
          "cdna_end": null,
          "cdna_length": 7053,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.5413G>A",
          "hgvs_p": "p.Val1805Met",
          "transcript": "ENST00000696649.1",
          "protein_id": "ENSP00000512780.1",
          "transcript_support_level": null,
          "aa_start": 1805,
          "aa_end": null,
          "aa_length": 1996,
          "cds_start": 5413,
          "cds_end": null,
          "cds_length": 5991,
          "cdna_start": 5442,
          "cdna_end": null,
          "cdna_length": 6902,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.4399G>A",
          "hgvs_p": "p.Val1467Met",
          "transcript": "ENST00000696664.1",
          "protein_id": "ENSP00000512790.1",
          "transcript_support_level": null,
          "aa_start": 1467,
          "aa_end": null,
          "aa_length": 1658,
          "cds_start": 4399,
          "cds_end": null,
          "cds_length": 4977,
          "cdna_start": 4401,
          "cdna_end": null,
          "cdna_length": 5812,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.4195G>A",
          "hgvs_p": "p.Val1399Met",
          "transcript": "ENST00000696683.1",
          "protein_id": "ENSP00000512806.1",
          "transcript_support_level": null,
          "aa_start": 1399,
          "aa_end": null,
          "aa_length": 1590,
          "cds_start": 4195,
          "cds_end": null,
          "cds_length": 4773,
          "cdna_start": 4197,
          "cdna_end": null,
          "cdna_length": 5657,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.3886G>A",
          "hgvs_p": "p.Val1296Met",
          "transcript": "ENST00000554809.6",
          "protein_id": "ENSP00000450632.2",
          "transcript_support_level": 2,
          "aa_start": 1296,
          "aa_end": null,
          "aa_length": 1487,
          "cds_start": 3886,
          "cds_end": null,
          "cds_length": 4464,
          "cdna_start": 3886,
          "cdna_end": null,
          "cdna_length": 4527,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.1345G>A",
          "hgvs_p": "p.Val449Met",
          "transcript": "ENST00000555484.2",
          "protein_id": "ENSP00000450797.2",
          "transcript_support_level": 3,
          "aa_start": 449,
          "aa_end": null,
          "aa_length": 572,
          "cds_start": 1345,
          "cds_end": null,
          "cds_length": 1719,
          "cdna_start": 1347,
          "cdna_end": null,
          "cdna_length": 1888,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.1345G>A",
          "hgvs_p": "p.Val449Met",
          "transcript": "ENST00000696665.1",
          "protein_id": "ENSP00000512791.1",
          "transcript_support_level": null,
          "aa_start": 449,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1345,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": 1347,
          "cdna_end": null,
          "cdna_length": 2467,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.5584G>A",
          "hgvs_p": "p.Val1862Met",
          "transcript": "XM_011537034.3",
          "protein_id": "XP_011535336.1",
          "transcript_support_level": null,
          "aa_start": 1862,
          "aa_end": null,
          "aa_length": 2053,
          "cds_start": 5584,
          "cds_end": null,
          "cds_length": 6162,
          "cdna_start": 5686,
          "cdna_end": null,
          "cdna_length": 7146,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.5506G>A",
          "hgvs_p": "p.Val1836Met",
          "transcript": "XM_011537035.4",
          "protein_id": "XP_011535337.1",
          "transcript_support_level": null,
          "aa_start": 1836,
          "aa_end": null,
          "aa_length": 2027,
          "cds_start": 5506,
          "cds_end": null,
          "cds_length": 6084,
          "cdna_start": 5608,
          "cdna_end": null,
          "cdna_length": 7068,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.4399G>A",
          "hgvs_p": "p.Val1467Met",
          "transcript": "XM_047431634.1",
          "protein_id": "XP_047287590.1",
          "transcript_support_level": null,
          "aa_start": 1467,
          "aa_end": null,
          "aa_length": 1658,
          "cds_start": 4399,
          "cds_end": null,
          "cds_length": 4977,
          "cdna_start": 4488,
          "cdna_end": null,
          "cdna_length": 5948,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "c.3598G>A",
          "hgvs_p": "p.Val1200Met",
          "transcript": "XM_011537037.4",
          "protein_id": "XP_011535339.1",
          "transcript_support_level": null,
          "aa_start": 1200,
          "aa_end": null,
          "aa_length": 1391,
          "cds_start": 3598,
          "cds_end": null,
          "cds_length": 4176,
          "cdna_start": 4157,
          "cdna_end": null,
          "cdna_length": 5617,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "n.1345G>A",
          "hgvs_p": null,
          "transcript": "ENST00000557110.2",
          "protein_id": "ENSP00000451846.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2090,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "n.1459G>A",
          "hgvs_p": null,
          "transcript": "ENST00000696645.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2220,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "n.*3594G>A",
          "hgvs_p": null,
          "transcript": "ENST00000696648.1",
          "protein_id": "ENSP00000512779.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 6349,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "n.2009G>A",
          "hgvs_p": null,
          "transcript": "ENST00000696651.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2706,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
          "gene_hgnc_id": 23168,
          "hgvs_c": "n.*1408G>A",
          "hgvs_p": null,
          "transcript": "ENST00000696663.1",
          "protein_id": "ENSP00000512789.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5907,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FANCM",
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      ],
      "gene_symbol": "FANCM",
      "gene_hgnc_id": 23168,
      "dbsnp": "rs144008013",
      "frequency_reference_population": 0.0007168446,
      "hom_count_reference_population": 3,
      "allele_count_reference_population": 1157,
      "gnomad_exomes_af": 0.000740208,
      "gnomad_genomes_af": 0.000492559,
      "gnomad_exomes_ac": 1082,
      "gnomad_genomes_ac": 75,
      "gnomad_exomes_homalt": 2,
      "gnomad_genomes_homalt": 1,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.15004777908325195,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.412,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.6005,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.09,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.935,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -7,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Moderate,BS1_Supporting,BS2",
      "acmg_by_gene": [
        {
          "score": -7,
          "benign_score": 7,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BS1_Supporting",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000267430.10",
          "gene_symbol": "FANCM",
          "hgnc_id": 23168,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.5569G>A",
          "hgvs_p": "p.Val1857Met"
        }
      ],
      "clinvar_disease": " complementation group M,FANCM-related disorder,Fanconi anemia,Hereditary cancer-predisposing syndrome,Premature ovarian failure 15,Spermatogenic failure 28,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:6 O:1",
      "phenotype_combined": "Fanconi anemia|Hereditary cancer-predisposing syndrome|Spermatogenic failure 28;Premature ovarian failure 15|Fanconi anemia, complementation group M|not provided|FANCM-related disorder",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}