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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-64225344-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=64225344&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 64225344,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000555002.6",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 116,
          "exon_rank_end": null,
          "exon_count": 116,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.20542C>T",
          "hgvs_p": "p.Arg6848Cys",
          "transcript": "NM_182914.3",
          "protein_id": "NP_878918.2",
          "transcript_support_level": null,
          "aa_start": 6848,
          "aa_end": null,
          "aa_length": 6907,
          "cds_start": 20542,
          "cds_end": null,
          "cds_length": 20724,
          "cdna_start": 20733,
          "cdna_end": null,
          "cdna_length": 21822,
          "mane_select": "ENST00000555002.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 116,
          "exon_rank_end": null,
          "exon_count": 116,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.20542C>T",
          "hgvs_p": "p.Arg6848Cys",
          "transcript": "ENST00000555002.6",
          "protein_id": "ENSP00000450831.2",
          "transcript_support_level": 1,
          "aa_start": 6848,
          "aa_end": null,
          "aa_length": 6907,
          "cds_start": 20542,
          "cds_end": null,
          "cds_length": 20724,
          "cdna_start": 20733,
          "cdna_end": null,
          "cdna_length": 21822,
          "mane_select": "NM_182914.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 115,
          "exon_rank_end": null,
          "exon_count": 115,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.20476C>T",
          "hgvs_p": "p.Arg6826Cys",
          "transcript": "ENST00000344113.8",
          "protein_id": "ENSP00000341781.4",
          "transcript_support_level": 1,
          "aa_start": 6826,
          "aa_end": null,
          "aa_length": 6885,
          "cds_start": 20476,
          "cds_end": null,
          "cds_length": 20658,
          "cdna_start": 20688,
          "cdna_end": null,
          "cdna_length": 21777,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.1489C>T",
          "hgvs_p": "p.Arg497Cys",
          "transcript": "ENST00000458046.6",
          "protein_id": "ENSP00000391937.2",
          "transcript_support_level": 1,
          "aa_start": 497,
          "aa_end": null,
          "aa_length": 556,
          "cds_start": 1489,
          "cds_end": null,
          "cds_length": 1671,
          "cdna_start": 1581,
          "cdna_end": null,
          "cdna_length": 2669,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 63,
          "exon_rank_end": null,
          "exon_count": 63,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "n.10009C>T",
          "hgvs_p": null,
          "transcript": "ENST00000394768.6",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 11095,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "n.1998C>T",
          "hgvs_p": null,
          "transcript": "ENST00000441438.2",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3084,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "n.2395C>T",
          "hgvs_p": null,
          "transcript": "ENST00000554805.6",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3481,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "n.2310C>T",
          "hgvs_p": null,
          "transcript": "ENST00000555022.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3396,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "n.*2297C>T",
          "hgvs_p": null,
          "transcript": "ENST00000555612.5",
          "protein_id": "ENSP00000451972.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6766,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "n.*2297C>T",
          "hgvs_p": null,
          "transcript": "ENST00000555612.5",
          "protein_id": "ENSP00000451972.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6766,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 116,
          "exon_rank_end": null,
          "exon_count": 116,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.20542C>T",
          "hgvs_p": "p.Arg6848Cys",
          "transcript": "ENST00000358025.7",
          "protein_id": "ENSP00000350719.3",
          "transcript_support_level": 5,
          "aa_start": 6848,
          "aa_end": null,
          "aa_length": 6907,
          "cds_start": 20542,
          "cds_end": null,
          "cds_length": 20724,
          "cdna_start": 20754,
          "cdna_end": null,
          "cdna_length": 21842,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 115,
          "exon_rank_end": null,
          "exon_count": 115,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.20476C>T",
          "hgvs_p": "p.Arg6826Cys",
          "transcript": "NM_015180.6",
          "protein_id": "NP_055995.4",
          "transcript_support_level": null,
          "aa_start": 6826,
          "aa_end": null,
          "aa_length": 6885,
          "cds_start": 20476,
          "cds_end": null,
          "cds_length": 20658,
          "cdna_start": 20667,
          "cdna_end": null,
          "cdna_length": 21756,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 115,
          "exon_rank_end": null,
          "exon_count": 115,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.20237C>T",
          "hgvs_p": "p.Ala6746Val",
          "transcript": "ENST00000554584.5",
          "protein_id": "ENSP00000452570.1",
          "transcript_support_level": 5,
          "aa_start": 6746,
          "aa_end": null,
          "aa_length": 6818,
          "cds_start": 20237,
          "cds_end": null,
          "cds_length": 20457,
          "cdna_start": 20288,
          "cdna_end": null,
          "cdna_length": 20508,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.1489C>T",
          "hgvs_p": "p.Arg497Cys",
          "transcript": "NM_182913.4",
          "protein_id": "NP_878917.1",
          "transcript_support_level": null,
          "aa_start": 497,
          "aa_end": null,
          "aa_length": 556,
          "cds_start": 1489,
          "cds_end": null,
          "cds_length": 1671,
          "cdna_start": 1581,
          "cdna_end": null,
          "cdna_length": 2670,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.1108C>T",
          "hgvs_p": "p.Arg370Cys",
          "transcript": "NM_182910.2",
          "protein_id": "NP_878914.1",
          "transcript_support_level": null,
          "aa_start": 370,
          "aa_end": null,
          "aa_length": 429,
          "cds_start": 1108,
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          "cds_length": 1290,
          "cdna_start": 1476,
          "cdna_end": null,
          "cdna_length": 2581,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 116,
          "exon_rank_end": null,
          "exon_count": 116,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.20629C>T",
          "hgvs_p": "p.Arg6877Cys",
          "transcript": "XM_011536574.2",
          "protein_id": "XP_011534876.1",
          "transcript_support_level": null,
          "aa_start": 6877,
          "aa_end": null,
          "aa_length": 6936,
          "cds_start": 20629,
          "cds_end": null,
          "cds_length": 20811,
          "cdna_start": 20820,
          "cdna_end": null,
          "cdna_length": 21925,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 116,
          "exon_rank_end": null,
          "exon_count": 116,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.20629C>T",
          "hgvs_p": "p.Arg6877Cys",
          "transcript": "XM_011536575.3",
          "protein_id": "XP_011534877.1",
          "transcript_support_level": null,
          "aa_start": 6877,
          "aa_end": null,
          "aa_length": 6936,
          "cds_start": 20629,
          "cds_end": null,
          "cds_length": 20811,
          "cdna_start": 20906,
          "cdna_end": null,
          "cdna_length": 22011,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 117,
          "exon_rank_end": null,
          "exon_count": 117,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.20629C>T",
          "hgvs_p": "p.Arg6877Cys",
          "transcript": "XM_011536576.3",
          "protein_id": "XP_011534878.1",
          "transcript_support_level": null,
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          "cds_length": 20811,
          "cdna_start": 21073,
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          "cdna_length": 22178,
          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 116,
          "exon_rank_end": null,
          "exon_count": 116,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.20629C>T",
          "hgvs_p": "p.Arg6877Cys",
          "transcript": "XM_011536577.3",
          "protein_id": "XP_011534879.1",
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          "cdna_start": 20757,
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          "cdna_length": 21862,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 116,
          "exon_rank_end": null,
          "exon_count": 116,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
          "hgvs_c": "c.20629C>T",
          "hgvs_p": "p.Arg6877Cys",
          "transcript": "XM_017021101.2",
          "protein_id": "XP_016876590.1",
          "transcript_support_level": null,
          "aa_start": 6877,
          "aa_end": null,
          "aa_length": 6936,
          "cds_start": 20629,
          "cds_end": null,
          "cds_length": 20811,
          "cdna_start": 30969,
          "cdna_end": null,
          "cdna_length": 32074,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 116,
          "exon_rank_end": null,
          "exon_count": 116,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE2",
          "gene_hgnc_id": 17084,
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        {
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          "gene_symbol": "ESR2",
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          "transcript": "ENST00000556275.5",
          "protein_id": "ENSP00000452485.2",
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        }
      ],
      "gene_symbol": "SYNE2",
      "gene_hgnc_id": 17084,
      "dbsnp": "rs201472187",
      "frequency_reference_population": 0.000032835433,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 53,
      "gnomad_exomes_af": 0.0000307832,
      "gnomad_genomes_af": 0.0000525362,
      "gnomad_exomes_ac": 45,
      "gnomad_genomes_ac": 8,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.22533249855041504,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.217,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1762,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.15,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 4.964,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -11,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Moderate,BP6,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -11,
          "benign_score": 11,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000555002.6",
          "gene_symbol": "SYNE2",
          "hgnc_id": 17084,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,Unknown",
          "hgvs_c": "c.20542C>T",
          "hgvs_p": "p.Arg6848Cys"
        },
        {
          "score": -3,
          "benign_score": 3,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000556275.5",
          "gene_symbol": "ESR2",
          "hgnc_id": 3468,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AD,AR,Unknown",
          "hgvs_c": "c.1406+9626G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " autosomal dominant,Emery-Dreifuss muscular dystrophy 5,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:2 LB:1",
      "phenotype_combined": "not provided|Emery-Dreifuss muscular dystrophy 5, autosomal dominant",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}