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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-64400861-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=64400861&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 64400861,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001364837.1",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "NM_005956.4",
          "protein_id": "NP_005947.3",
          "transcript_support_level": null,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 935,
          "cds_start": 110,
          "cds_end": null,
          "cds_length": 2808,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000652337.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005956.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000652337.1",
          "protein_id": "ENSP00000498336.1",
          "transcript_support_level": null,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 935,
          "cds_start": 110,
          "cds_end": null,
          "cds_length": 2808,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_005956.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000652337.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "n.227G>A",
          "hgvs_p": null,
          "transcript": "ENST00000555252.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000555252.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "NM_001364837.1",
          "protein_id": "NP_001351766.1",
          "transcript_support_level": null,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 964,
          "cds_start": 110,
          "cds_end": null,
          "cds_length": 2895,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001364837.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000545908.6",
          "protein_id": "ENSP00000438588.2",
          "transcript_support_level": 2,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 964,
          "cds_start": 110,
          "cds_end": null,
          "cds_length": 2895,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000545908.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000900031.1",
          "protein_id": "ENSP00000570090.1",
          "transcript_support_level": null,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 962,
          "cds_start": 110,
          "cds_end": null,
          "cds_length": 2889,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000900031.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000940490.1",
          "protein_id": "ENSP00000610549.1",
          "transcript_support_level": null,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 952,
          "cds_start": 110,
          "cds_end": null,
          "cds_length": 2859,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940490.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000940495.1",
          "protein_id": "ENSP00000610554.1",
          "transcript_support_level": null,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 935,
          "cds_start": 110,
          "cds_end": null,
          "cds_length": 2808,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940495.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000941180.1",
          "protein_id": "ENSP00000611239.1",
          "transcript_support_level": null,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 935,
          "cds_start": 110,
          "cds_end": null,
          "cds_length": 2808,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000941180.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000900023.1",
          "protein_id": "ENSP00000570082.1",
          "transcript_support_level": null,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 932,
          "cds_start": 110,
          "cds_end": null,
          "cds_length": 2799,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000900023.1"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000940488.1",
          "protein_id": "ENSP00000610547.1",
          "transcript_support_level": null,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 926,
          "cds_start": 110,
          "cds_end": null,
          "cds_length": 2781,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940488.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
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          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000557370.3",
          "protein_id": "ENSP00000477199.2",
          "transcript_support_level": 2,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 922,
          "cds_start": 110,
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          "cds_length": 2769,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000557370.3"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
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          "exon_count": 27,
          "intron_rank": null,
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          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000900025.1",
          "protein_id": "ENSP00000570084.1",
          "transcript_support_level": null,
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          "cds_start": 110,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000940494.1",
          "protein_id": "ENSP00000610553.1",
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          "cds_start": 110,
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          "cdna_start": null,
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        {
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          ],
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          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000940489.1",
          "protein_id": "ENSP00000610548.1",
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          "cds_end": null,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000940489.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000697168.1",
          "protein_id": "ENSP00000513156.1",
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          "aa_length": 884,
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          "cdna_start": null,
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        {
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          "strand": true,
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          "exon_count": 27,
          "intron_rank": null,
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          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000940486.1",
          "protein_id": "ENSP00000610545.1",
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        {
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          "gene_symbol": "MTHFD1",
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          "hgvs_c": "c.110G>A",
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        {
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          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
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          "transcript": "ENST00000900024.1",
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          "biotype": "protein_coding",
          "feature": "ENST00000900024.1"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFD1",
          "gene_hgnc_id": 7432,
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His",
          "transcript": "ENST00000697174.1",
          "protein_id": "ENSP00000513160.1",
          "transcript_support_level": null,
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          "cds_start": 110,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000697174.1"
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        {
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000697176.1"
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      ],
      "gene_symbol": "MTHFD1",
      "gene_hgnc_id": 7432,
      "dbsnp": "rs905506733",
      "frequency_reference_population": 0.000006831611,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 11,
      "gnomad_exomes_af": 0.00000480099,
      "gnomad_genomes_af": 0.0000262933,
      "gnomad_exomes_ac": 7,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.34263184666633606,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.093,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0985,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.56,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.687,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001364837.1",
          "gene_symbol": "MTHFD1",
          "hgnc_id": 7432,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.110G>A",
          "hgvs_p": "p.Arg37His"
        }
      ],
      "clinvar_disease": "Inborn genetic diseases,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "not provided|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}