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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-64749430-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=64749430&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 64749430,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001024858.4",
      "consequences": [
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.6863A>C",
          "hgvs_p": "p.Asn2288Thr",
          "transcript": "NM_001355436.2",
          "protein_id": "NP_001342365.1",
          "transcript_support_level": null,
          "aa_start": 2288,
          "aa_end": null,
          "aa_length": 2328,
          "cds_start": 6863,
          "cds_end": null,
          "cds_length": 6987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000644917.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001355436.2"
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.6863A>C",
          "hgvs_p": "p.Asn2288Thr",
          "transcript": "ENST00000644917.1",
          "protein_id": "ENSP00000495909.1",
          "transcript_support_level": null,
          "aa_start": 2288,
          "aa_end": null,
          "aa_length": 2328,
          "cds_start": 6863,
          "cds_end": null,
          "cds_length": 6987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001355436.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000644917.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.2963A>C",
          "hgvs_p": "p.Asn988Thr",
          "transcript": "ENST00000553938.5",
          "protein_id": "ENSP00000451324.1",
          "transcript_support_level": 1,
          "aa_start": 988,
          "aa_end": null,
          "aa_length": 1028,
          "cds_start": 2963,
          "cds_end": null,
          "cds_length": 3087,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000553938.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHG3",
          "gene_hgnc_id": 20364,
          "hgvs_c": "c.*5727T>G",
          "hgvs_p": null,
          "transcript": "NM_001308147.2",
          "protein_id": "NP_001295076.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1219,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3660,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000247226.13",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001308147.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHG3",
          "gene_hgnc_id": 20364,
          "hgvs_c": "c.*5727T>G",
          "hgvs_p": null,
          "transcript": "ENST00000247226.13",
          "protein_id": "ENSP00000247226.8",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1219,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3660,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001308147.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000247226.13"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHG3",
          "gene_hgnc_id": 20364,
          "hgvs_c": "c.*5727T>G",
          "hgvs_p": null,
          "transcript": "ENST00000634379.2",
          "protein_id": "ENSP00000489373.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1240,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3723,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000634379.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "n.600A>C",
          "hgvs_p": null,
          "transcript": "ENST00000342835.9",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000342835.9"
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 35,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.6863A>C",
          "hgvs_p": "p.Asn2288Thr",
          "transcript": "NM_001024858.4",
          "protein_id": "NP_001020029.1",
          "transcript_support_level": null,
          "aa_start": 2288,
          "aa_end": null,
          "aa_length": 2328,
          "cds_start": 6863,
          "cds_end": null,
          "cds_length": 6987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001024858.4"
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 35,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.6863A>C",
          "hgvs_p": "p.Asn2288Thr",
          "transcript": "ENST00000389722.7",
          "protein_id": "ENSP00000374372.3",
          "transcript_support_level": 2,
          "aa_start": 2288,
          "aa_end": null,
          "aa_length": 2328,
          "cds_start": 6863,
          "cds_end": null,
          "cds_length": 6987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000389722.7"
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 37,
          "exon_rank_end": null,
          "exon_count": 37,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.6863A>C",
          "hgvs_p": "p.Asn2288Thr",
          "transcript": "ENST00000961380.1",
          "protein_id": "ENSP00000631439.1",
          "transcript_support_level": null,
          "aa_start": 2288,
          "aa_end": null,
          "aa_length": 2328,
          "cds_start": 6863,
          "cds_end": null,
          "cds_length": 6987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961380.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.6863A>C",
          "hgvs_p": "p.Asn2288Thr",
          "transcript": "ENST00000961381.1",
          "protein_id": "ENSP00000631440.1",
          "transcript_support_level": null,
          "aa_start": 2288,
          "aa_end": null,
          "aa_length": 2328,
          "cds_start": 6863,
          "cds_end": null,
          "cds_length": 6987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961381.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.6863A>C",
          "hgvs_p": "p.Asn2288Thr",
          "transcript": "ENST00000961382.1",
          "protein_id": "ENSP00000631441.1",
          "transcript_support_level": null,
          "aa_start": 2288,
          "aa_end": null,
          "aa_length": 2328,
          "cds_start": 6863,
          "cds_end": null,
          "cds_length": 6987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961382.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 35,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.6782A>C",
          "hgvs_p": "p.Asn2261Thr",
          "transcript": "ENST00000902733.1",
          "protein_id": "ENSP00000572792.1",
          "transcript_support_level": null,
          "aa_start": 2261,
          "aa_end": null,
          "aa_length": 2301,
          "cds_start": 6782,
          "cds_end": null,
          "cds_length": 6906,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902733.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 37,
          "exon_rank_end": null,
          "exon_count": 37,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.6863A>C",
          "hgvs_p": "p.Asn2288Thr",
          "transcript": "XM_017021612.3",
          "protein_id": "XP_016877101.1",
          "transcript_support_level": null,
          "aa_start": 2288,
          "aa_end": null,
          "aa_length": 2328,
          "cds_start": 6863,
          "cds_end": null,
          "cds_length": 6987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017021612.3"
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.6863A>C",
          "hgvs_p": "p.Asn2288Thr",
          "transcript": "XM_024449699.2",
          "protein_id": "XP_024305467.1",
          "transcript_support_level": null,
          "aa_start": 2288,
          "aa_end": null,
          "aa_length": 2328,
          "cds_start": 6863,
          "cds_end": null,
          "cds_length": 6987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024449699.2"
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 37,
          "exon_rank_end": null,
          "exon_count": 37,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.6863A>C",
          "hgvs_p": "p.Asn2288Thr",
          "transcript": "XM_047431724.1",
          "protein_id": "XP_047287680.1",
          "transcript_support_level": null,
          "aa_start": 2288,
          "aa_end": null,
          "aa_length": 2328,
          "cds_start": 6863,
          "cds_end": null,
          "cds_length": 6987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047431724.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "c.6863A>C",
          "hgvs_p": "p.Asn2288Thr",
          "transcript": "XM_047431725.1",
          "protein_id": "XP_047287681.1",
          "transcript_support_level": null,
          "aa_start": 2288,
          "aa_end": null,
          "aa_length": 2328,
          "cds_start": 6863,
          "cds_end": null,
          "cds_length": 6987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047431725.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPTB",
          "gene_hgnc_id": 11274,
          "hgvs_c": "n.4266A>C",
          "hgvs_p": null,
          "transcript": "ENST00000556227.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000556227.1"
        }
      ],
      "gene_symbol": "SPTB",
      "gene_hgnc_id": 11274,
      "dbsnp": "rs765605676",
      "frequency_reference_population": 0.0000062318886,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 10,
      "gnomad_exomes_af": 0.00000619619,
      "gnomad_genomes_af": 0.00000657272,
      "gnomad_exomes_ac": 9,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0967138409614563,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.093,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0743,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.75,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.247,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001024858.4",
          "gene_symbol": "SPTB",
          "hgnc_id": 11274,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.6863A>C",
          "hgvs_p": "p.Asn2288Thr"
        },
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001308147.2",
          "gene_symbol": "PLEKHG3",
          "hgnc_id": 20364,
          "effects": [
            "3_prime_UTR_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.*5727T>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}