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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-70326416-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=70326416&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 70326416,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001202547.2",
      "consequences": [
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "c.238A>G",
          "hgvs_p": "p.Asn80Asp",
          "transcript": "NM_016468.7",
          "protein_id": "NP_057552.1",
          "transcript_support_level": null,
          "aa_start": 80,
          "aa_end": null,
          "aa_length": 106,
          "cds_start": 238,
          "cds_end": null,
          "cds_length": 321,
          "cdna_start": 334,
          "cdna_end": null,
          "cdna_length": 1669,
          "mane_select": "ENST00000389912.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_016468.7"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "c.238A>G",
          "hgvs_p": "p.Asn80Asp",
          "transcript": "ENST00000389912.7",
          "protein_id": "ENSP00000374562.5",
          "transcript_support_level": 1,
          "aa_start": 80,
          "aa_end": null,
          "aa_length": 106,
          "cds_start": 238,
          "cds_end": null,
          "cds_length": 321,
          "cdna_start": 334,
          "cdna_end": null,
          "cdna_length": 1669,
          "mane_select": "NM_016468.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000389912.7"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNJ2BP-COX16",
          "gene_hgnc_id": 48350,
          "hgvs_c": "c.466A>G",
          "hgvs_p": "p.Asn156Asp",
          "transcript": "ENST00000621525.4",
          "protein_id": "ENSP00000482133.1",
          "transcript_support_level": 2,
          "aa_start": 156,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 466,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": 577,
          "cdna_end": null,
          "cdna_length": 929,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000621525.4"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNJ2BP-COX16",
          "gene_hgnc_id": 48350,
          "hgvs_c": "c.493A>G",
          "hgvs_p": "p.Asn165Asp",
          "transcript": "NM_001202547.2",
          "protein_id": "NP_001189476.1",
          "transcript_support_level": null,
          "aa_start": 165,
          "aa_end": null,
          "aa_length": 191,
          "cds_start": 493,
          "cds_end": null,
          "cds_length": 576,
          "cdna_start": 620,
          "cdna_end": null,
          "cdna_length": 1955,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001202547.2"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNJ2BP-COX16",
          "gene_hgnc_id": 48350,
          "hgvs_c": "c.466A>G",
          "hgvs_p": "p.Asn156Asp",
          "transcript": "NM_001202548.2",
          "protein_id": "NP_001189477.1",
          "transcript_support_level": null,
          "aa_start": 156,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 466,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": 593,
          "cdna_end": null,
          "cdna_length": 1928,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001202548.2"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNJ2BP-COX16",
          "gene_hgnc_id": 48350,
          "hgvs_c": "c.394A>G",
          "hgvs_p": "p.Asn132Asp",
          "transcript": "NM_001202549.2",
          "protein_id": "NP_001189478.1",
          "transcript_support_level": null,
          "aa_start": 132,
          "aa_end": null,
          "aa_length": 158,
          "cds_start": 394,
          "cds_end": null,
          "cds_length": 477,
          "cdna_start": 521,
          "cdna_end": null,
          "cdna_length": 1856,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001202549.2"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNJ2BP-COX16",
          "gene_hgnc_id": 48350,
          "hgvs_c": "c.394A>G",
          "hgvs_p": "p.Asn132Asp",
          "transcript": "ENST00000617124.4",
          "protein_id": "ENSP00000484161.1",
          "transcript_support_level": 2,
          "aa_start": 132,
          "aa_end": null,
          "aa_length": 151,
          "cds_start": 394,
          "cds_end": null,
          "cds_length": 458,
          "cdna_start": 415,
          "cdna_end": null,
          "cdna_length": 479,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000617124.4"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "c.355A>G",
          "hgvs_p": "p.Asn119Asp",
          "transcript": "ENST00000854462.1",
          "protein_id": "ENSP00000524521.1",
          "transcript_support_level": null,
          "aa_start": 119,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 355,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": 499,
          "cdna_end": null,
          "cdna_length": 1832,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854462.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "c.316A>G",
          "hgvs_p": "p.Asn106Asp",
          "transcript": "ENST00000854465.1",
          "protein_id": "ENSP00000524524.1",
          "transcript_support_level": null,
          "aa_start": 106,
          "aa_end": null,
          "aa_length": 132,
          "cds_start": 316,
          "cds_end": null,
          "cds_length": 399,
          "cdna_start": 410,
          "cdna_end": null,
          "cdna_length": 753,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854465.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "c.283A>G",
          "hgvs_p": "p.Asn95Asp",
          "transcript": "ENST00000854463.1",
          "protein_id": "ENSP00000524522.1",
          "transcript_support_level": null,
          "aa_start": 95,
          "aa_end": null,
          "aa_length": 121,
          "cds_start": 283,
          "cds_end": null,
          "cds_length": 366,
          "cdna_start": 398,
          "cdna_end": null,
          "cdna_length": 741,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854463.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "c.244A>G",
          "hgvs_p": "p.Asn82Asp",
          "transcript": "ENST00000854466.1",
          "protein_id": "ENSP00000524525.1",
          "transcript_support_level": null,
          "aa_start": 82,
          "aa_end": null,
          "aa_length": 108,
          "cds_start": 244,
          "cds_end": null,
          "cds_length": 327,
          "cdna_start": 334,
          "cdna_end": null,
          "cdna_length": 677,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854466.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "c.175A>G",
          "hgvs_p": "p.Asn59Asp",
          "transcript": "ENST00000940438.1",
          "protein_id": "ENSP00000610497.1",
          "transcript_support_level": null,
          "aa_start": 59,
          "aa_end": null,
          "aa_length": 85,
          "cds_start": 175,
          "cds_end": null,
          "cds_length": 258,
          "cdna_start": 267,
          "cdna_end": null,
          "cdna_length": 615,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940438.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "c.166A>G",
          "hgvs_p": "p.Asn56Asp",
          "transcript": "NM_001204090.2",
          "protein_id": "NP_001191019.1",
          "transcript_support_level": null,
          "aa_start": 56,
          "aa_end": null,
          "aa_length": 82,
          "cds_start": 166,
          "cds_end": null,
          "cds_length": 249,
          "cdna_start": 262,
          "cdna_end": null,
          "cdna_length": 1597,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001204090.2"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "c.103A>G",
          "hgvs_p": "p.Asn35Asp",
          "transcript": "ENST00000854464.1",
          "protein_id": "ENSP00000524523.1",
          "transcript_support_level": null,
          "aa_start": 35,
          "aa_end": null,
          "aa_length": 61,
          "cds_start": 103,
          "cds_end": null,
          "cds_length": 186,
          "cdna_start": 215,
          "cdna_end": null,
          "cdna_length": 558,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854464.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "c.97A>G",
          "hgvs_p": "p.Asn33Asp",
          "transcript": "ENST00000555276.5",
          "protein_id": "ENSP00000480746.1",
          "transcript_support_level": 2,
          "aa_start": 33,
          "aa_end": null,
          "aa_length": 52,
          "cds_start": 97,
          "cds_end": null,
          "cds_length": 161,
          "cdna_start": 254,
          "cdna_end": null,
          "cdna_length": 318,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000555276.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "n.99A>G",
          "hgvs_p": null,
          "transcript": "ENST00000554366.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 447,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000554366.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "n.250A>G",
          "hgvs_p": null,
          "transcript": "ENST00000555601.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 384,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000555601.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COX16",
          "gene_hgnc_id": 20213,
          "hgvs_c": "n.279A>G",
          "hgvs_p": null,
          "transcript": "ENST00000557612.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 627,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000557612.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNJ2BP-COX16",
          "gene_hgnc_id": 48350,
          "hgvs_c": "n.514A>G",
          "hgvs_p": null,
          "transcript": "ENST00000617196.4",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 580,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000617196.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000259033",
          "gene_hgnc_id": null,
          "hgvs_c": "n.713-16256T>C",
          "hgvs_p": null,
          "transcript": "ENST00000655620.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1429,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000655620.1"
        }
      ],
      "gene_symbol": "SYNJ2BP-COX16",
      "gene_hgnc_id": 48350,
      "dbsnp": "rs1886077590",
      "frequency_reference_population": 0.0000034686236,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.00000346862,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 5,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.4522066116333008,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.277,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.7501,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.23,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.117,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001202547.2",
          "gene_symbol": "SYNJ2BP-COX16",
          "hgnc_id": 48350,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.493A>G",
          "hgvs_p": "p.Asn165Asp"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_016468.7",
          "gene_symbol": "COX16",
          "hgnc_id": 20213,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.238A>G",
          "hgvs_p": "p.Asn80Asp"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000655620.1",
          "gene_symbol": "ENSG00000259033",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.713-16256T>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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