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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-74060647-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=74060647&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 74060647,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_005589.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.1603C>T",
          "hgvs_p": "p.Arg535Cys",
          "transcript": "NM_005589.4",
          "protein_id": "NP_005580.1",
          "transcript_support_level": null,
          "aa_start": 535,
          "aa_end": null,
          "aa_length": 535,
          "cds_start": 1603,
          "cds_end": null,
          "cds_length": 1608,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000553458.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005589.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.1603C>T",
          "hgvs_p": "p.Arg535Cys",
          "transcript": "ENST00000553458.6",
          "protein_id": "ENSP00000450436.1",
          "transcript_support_level": 1,
          "aa_start": 535,
          "aa_end": null,
          "aa_length": 535,
          "cds_start": 1603,
          "cds_end": null,
          "cds_length": 1608,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_005589.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000553458.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 11,
          "intron_rank_end": null,
          "gene_symbol": "BBOF1",
          "gene_hgnc_id": 19855,
          "hgvs_c": "c.1578+3389G>A",
          "hgvs_p": null,
          "transcript": "NM_025057.3",
          "protein_id": "NP_079333.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 529,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1590,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000394009.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_025057.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 11,
          "intron_rank_end": null,
          "gene_symbol": "BBOF1",
          "gene_hgnc_id": 19855,
          "hgvs_c": "c.1578+3389G>A",
          "hgvs_p": null,
          "transcript": "ENST00000394009.5",
          "protein_id": "ENSP00000377577.3",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 529,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1590,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_025057.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000394009.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "n.1821C>T",
          "hgvs_p": null,
          "transcript": "ENST00000554501.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000554501.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.1600C>T",
          "hgvs_p": "p.Arg534Cys",
          "transcript": "ENST00000931795.1",
          "protein_id": "ENSP00000601854.1",
          "transcript_support_level": null,
          "aa_start": 534,
          "aa_end": null,
          "aa_length": 534,
          "cds_start": 1600,
          "cds_end": null,
          "cds_length": 1605,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931795.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.1588C>T",
          "hgvs_p": "p.Arg530Cys",
          "transcript": "ENST00000901413.1",
          "protein_id": "ENSP00000571472.1",
          "transcript_support_level": null,
          "aa_start": 530,
          "aa_end": null,
          "aa_length": 530,
          "cds_start": 1588,
          "cds_end": null,
          "cds_length": 1593,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901413.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.1564C>T",
          "hgvs_p": "p.Arg522Cys",
          "transcript": "NM_001278593.2",
          "protein_id": "NP_001265522.1",
          "transcript_support_level": null,
          "aa_start": 522,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 1564,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001278593.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.1564C>T",
          "hgvs_p": "p.Arg522Cys",
          "transcript": "ENST00000350259.8",
          "protein_id": "ENSP00000342564.4",
          "transcript_support_level": 2,
          "aa_start": 522,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 1564,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000350259.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.1555C>T",
          "hgvs_p": "p.Arg519Cys",
          "transcript": "ENST00000901410.1",
          "protein_id": "ENSP00000571469.1",
          "transcript_support_level": null,
          "aa_start": 519,
          "aa_end": null,
          "aa_length": 519,
          "cds_start": 1555,
          "cds_end": null,
          "cds_length": 1560,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901410.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.1423C>T",
          "hgvs_p": "p.Arg475Cys",
          "transcript": "ENST00000901407.1",
          "protein_id": "ENSP00000571466.1",
          "transcript_support_level": null,
          "aa_start": 475,
          "aa_end": null,
          "aa_length": 475,
          "cds_start": 1423,
          "cds_end": null,
          "cds_length": 1428,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901407.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.1291C>T",
          "hgvs_p": "p.Arg431Cys",
          "transcript": "ENST00000901411.1",
          "protein_id": "ENSP00000571470.1",
          "transcript_support_level": null,
          "aa_start": 431,
          "aa_end": null,
          "aa_length": 431,
          "cds_start": 1291,
          "cds_end": null,
          "cds_length": 1296,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901411.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.1141C>T",
          "hgvs_p": "p.Arg381Cys",
          "transcript": "NM_001278594.2",
          "protein_id": "NP_001265523.1",
          "transcript_support_level": null,
          "aa_start": 381,
          "aa_end": null,
          "aa_length": 381,
          "cds_start": 1141,
          "cds_end": null,
          "cds_length": 1146,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001278594.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.1120C>T",
          "hgvs_p": "p.Arg374Cys",
          "transcript": "ENST00000901412.1",
          "protein_id": "ENSP00000571471.1",
          "transcript_support_level": null,
          "aa_start": 374,
          "aa_end": null,
          "aa_length": 374,
          "cds_start": 1120,
          "cds_end": null,
          "cds_length": 1125,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901412.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.1099C>T",
          "hgvs_p": "p.Arg367Cys",
          "transcript": "ENST00000901408.1",
          "protein_id": "ENSP00000571467.1",
          "transcript_support_level": null,
          "aa_start": 367,
          "aa_end": null,
          "aa_length": 367,
          "cds_start": 1099,
          "cds_end": null,
          "cds_length": 1104,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901408.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.862C>T",
          "hgvs_p": "p.Arg288Cys",
          "transcript": "ENST00000901409.1",
          "protein_id": "ENSP00000571468.1",
          "transcript_support_level": null,
          "aa_start": 288,
          "aa_end": null,
          "aa_length": 288,
          "cds_start": 862,
          "cds_end": null,
          "cds_length": 867,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901409.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.757C>T",
          "hgvs_p": "p.Arg253Cys",
          "transcript": "ENST00000960461.1",
          "protein_id": "ENSP00000630520.1",
          "transcript_support_level": null,
          "aa_start": 253,
          "aa_end": null,
          "aa_length": 253,
          "cds_start": 757,
          "cds_end": null,
          "cds_length": 762,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000960461.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH6A1",
          "gene_hgnc_id": 7179,
          "hgvs_c": "c.754C>T",
          "hgvs_p": "p.Arg252Cys",
          "transcript": "ENST00000555126.1",
          "protein_id": "ENSP00000452081.1",
          "transcript_support_level": 2,
          "aa_start": 252,
          "aa_end": null,
          "aa_length": 252,
          "cds_start": 754,
          "cds_end": null,
          "cds_length": 759,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000555126.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 10,
          "intron_rank_end": null,
          "gene_symbol": "BBOF1",
          "gene_hgnc_id": 19855,
          "hgvs_c": "c.1434+3389G>A",
          "hgvs_p": null,
          "transcript": "ENST00000901146.1",
          "protein_id": "ENSP00000571205.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 481,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1446,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901146.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "BBOF1",
          "gene_hgnc_id": 19855,
          "hgvs_c": "c.939+3389G>A",
          "hgvs_p": null,
          "transcript": "ENST00000901145.1",
          "protein_id": "ENSP00000571204.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 316,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 951,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
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      "gnomad_exomes_homalt": 0,
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      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Uncertain_significance",
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      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.17,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.566,
      "phylop100way_prediction": "Uncertain_significance",
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      "spliceai_max_prediction": "Benign",
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      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP5",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
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            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_005589.4",
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        {
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          "verdict": "Uncertain_significance",
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      "clinvar_disease": "Methylmalonate semialdehyde dehydrogenase deficiency",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Methylmalonate semialdehyde dehydrogenase deficiency",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
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  ],
  "message": null
}