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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-90894459-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=90894459&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 90894459,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_004755.4",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1598G>A",
          "hgvs_p": "p.Ser533Asn",
          "transcript": "NM_004755.4",
          "protein_id": "NP_004746.2",
          "transcript_support_level": null,
          "aa_start": 533,
          "aa_end": null,
          "aa_length": 802,
          "cds_start": 1598,
          "cds_end": null,
          "cds_length": 2409,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000614987.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004755.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1598G>A",
          "hgvs_p": "p.Ser533Asn",
          "transcript": "ENST00000614987.5",
          "protein_id": "ENSP00000479667.1",
          "transcript_support_level": 1,
          "aa_start": 533,
          "aa_end": null,
          "aa_length": 802,
          "cds_start": 1598,
          "cds_end": null,
          "cds_length": 2409,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_004755.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000614987.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1598G>A",
          "hgvs_p": "p.Ser533Asn",
          "transcript": "ENST00000418736.6",
          "protein_id": "ENSP00000402787.2",
          "transcript_support_level": 1,
          "aa_start": 533,
          "aa_end": null,
          "aa_length": 549,
          "cds_start": 1598,
          "cds_end": null,
          "cds_length": 1650,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000418736.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1631G>A",
          "hgvs_p": "p.Ser544Asn",
          "transcript": "ENST00000886639.1",
          "protein_id": "ENSP00000556698.1",
          "transcript_support_level": null,
          "aa_start": 544,
          "aa_end": null,
          "aa_length": 813,
          "cds_start": 1631,
          "cds_end": null,
          "cds_length": 2442,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000886639.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1598G>A",
          "hgvs_p": "p.Ser533Asn",
          "transcript": "NM_001322229.2",
          "protein_id": "NP_001309158.1",
          "transcript_support_level": null,
          "aa_start": 533,
          "aa_end": null,
          "aa_length": 795,
          "cds_start": 1598,
          "cds_end": null,
          "cds_length": 2388,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001322229.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1598G>A",
          "hgvs_p": "p.Ser533Asn",
          "transcript": "ENST00000886636.1",
          "protein_id": "ENSP00000556695.1",
          "transcript_support_level": null,
          "aa_start": 533,
          "aa_end": null,
          "aa_length": 795,
          "cds_start": 1598,
          "cds_end": null,
          "cds_length": 2388,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000886636.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1514G>A",
          "hgvs_p": "p.Ser505Asn",
          "transcript": "NM_001322236.2",
          "protein_id": "NP_001309165.1",
          "transcript_support_level": null,
          "aa_start": 505,
          "aa_end": null,
          "aa_length": 774,
          "cds_start": 1514,
          "cds_end": null,
          "cds_length": 2325,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001322236.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1514G>A",
          "hgvs_p": "p.Ser505Asn",
          "transcript": "ENST00000946930.1",
          "protein_id": "ENSP00000616989.1",
          "transcript_support_level": null,
          "aa_start": 505,
          "aa_end": null,
          "aa_length": 774,
          "cds_start": 1514,
          "cds_end": null,
          "cds_length": 2325,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946930.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1526G>A",
          "hgvs_p": "p.Ser509Asn",
          "transcript": "ENST00000946932.1",
          "protein_id": "ENSP00000616991.1",
          "transcript_support_level": null,
          "aa_start": 509,
          "aa_end": null,
          "aa_length": 771,
          "cds_start": 1526,
          "cds_end": null,
          "cds_length": 2316,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946932.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1490G>A",
          "hgvs_p": "p.Ser497Asn",
          "transcript": "NM_001322228.2",
          "protein_id": "NP_001309157.1",
          "transcript_support_level": null,
          "aa_start": 497,
          "aa_end": null,
          "aa_length": 766,
          "cds_start": 1490,
          "cds_end": null,
          "cds_length": 2301,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001322228.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1490G>A",
          "hgvs_p": "p.Ser497Asn",
          "transcript": "ENST00000886635.1",
          "protein_id": "ENSP00000556694.1",
          "transcript_support_level": null,
          "aa_start": 497,
          "aa_end": null,
          "aa_length": 766,
          "cds_start": 1490,
          "cds_end": null,
          "cds_length": 2301,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000886635.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1436G>A",
          "hgvs_p": "p.Ser479Asn",
          "transcript": "NM_001322233.2",
          "protein_id": "NP_001309162.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 748,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2247,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001322233.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1436G>A",
          "hgvs_p": "p.Ser479Asn",
          "transcript": "ENST00000886638.1",
          "protein_id": "ENSP00000556697.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 748,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2247,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000886638.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1379G>A",
          "hgvs_p": "p.Ser460Asn",
          "transcript": "NM_001322232.2",
          "protein_id": "NP_001309161.1",
          "transcript_support_level": null,
          "aa_start": 460,
          "aa_end": null,
          "aa_length": 729,
          "cds_start": 1379,
          "cds_end": null,
          "cds_length": 2190,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001322232.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1379G>A",
          "hgvs_p": "p.Ser460Asn",
          "transcript": "ENST00000946931.1",
          "protein_id": "ENSP00000616990.1",
          "transcript_support_level": null,
          "aa_start": 460,
          "aa_end": null,
          "aa_length": 729,
          "cds_start": 1379,
          "cds_end": null,
          "cds_length": 2190,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946931.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1361G>A",
          "hgvs_p": "p.Ser454Asn",
          "transcript": "NM_001322235.2",
          "protein_id": "NP_001309164.1",
          "transcript_support_level": null,
          "aa_start": 454,
          "aa_end": null,
          "aa_length": 723,
          "cds_start": 1361,
          "cds_end": null,
          "cds_length": 2172,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001322235.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1361G>A",
          "hgvs_p": "p.Ser454Asn",
          "transcript": "NM_001322237.2",
          "protein_id": "NP_001309166.1",
          "transcript_support_level": null,
          "aa_start": 454,
          "aa_end": null,
          "aa_length": 723,
          "cds_start": 1361,
          "cds_end": null,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001322237.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1361G>A",
          "hgvs_p": "p.Ser454Asn",
          "transcript": "ENST00000536315.6",
          "protein_id": "ENSP00000442803.2",
          "transcript_support_level": 2,
          "aa_start": 454,
          "aa_end": null,
          "aa_length": 723,
          "cds_start": 1361,
          "cds_end": null,
          "cds_length": 2172,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000536315.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1307G>A",
          "hgvs_p": "p.Ser436Asn",
          "transcript": "ENST00000946933.1",
          "protein_id": "ENSP00000616992.1",
          "transcript_support_level": null,
          "aa_start": 436,
          "aa_end": null,
          "aa_length": 705,
          "cds_start": 1307,
          "cds_end": null,
          "cds_length": 2118,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946933.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA5",
          "gene_hgnc_id": 10434,
          "hgvs_c": "c.1022G>A",
          "hgvs_p": "p.Ser341Asn",
          "transcript": "NM_001322234.2",
          "protein_id": "NP_001309163.1",
          "transcript_support_level": null,
          "aa_start": 341,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1022,
          "cds_end": null,
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        {
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        {
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000648062.1"
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      ],
      "gene_symbol": "RPS6KA5",
      "gene_hgnc_id": 10434,
      "dbsnp": null,
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.43022459745407104,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.087,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.5724,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.29,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 6.081,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_004755.4",
          "gene_symbol": "RPS6KA5",
          "hgnc_id": 10434,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1598G>A",
          "hgvs_p": "p.Ser533Asn"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}