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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-94381078-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=94381078&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 94381078,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000393087.9",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "NM_000295.5",
          "protein_id": "NP_000286.3",
          "transcript_support_level": null,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 757,
          "cdna_end": null,
          "cdna_length": 3006,
          "mane_select": "ENST00000393087.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "ENST00000393087.9",
          "protein_id": "ENSP00000376802.4",
          "transcript_support_level": 1,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 757,
          "cdna_end": null,
          "cdna_length": 3006,
          "mane_select": "NM_000295.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "ENST00000355814.8",
          "protein_id": "ENSP00000348068.4",
          "transcript_support_level": 1,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 987,
          "cdna_end": null,
          "cdna_length": 3236,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "ENST00000393088.8",
          "protein_id": "ENSP00000376803.4",
          "transcript_support_level": 1,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 1265,
          "cdna_end": null,
          "cdna_length": 1885,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "ENST00000404814.8",
          "protein_id": "ENSP00000385960.4",
          "transcript_support_level": 1,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 1021,
          "cdna_end": null,
          "cdna_length": 1628,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "ENST00000437397.5",
          "protein_id": "ENSP00000408474.1",
          "transcript_support_level": 1,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 1091,
          "cdna_end": null,
          "cdna_length": 3340,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "ENST00000440909.5",
          "protein_id": "ENSP00000390299.1",
          "transcript_support_level": 1,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 895,
          "cdna_end": null,
          "cdna_length": 3144,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "ENST00000448921.5",
          "protein_id": "ENSP00000416066.1",
          "transcript_support_level": 1,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 1283,
          "cdna_end": null,
          "cdna_length": 3532,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "ENST00000449399.7",
          "protein_id": "ENSP00000416354.3",
          "transcript_support_level": 1,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 1012,
          "cdna_end": null,
          "cdna_length": 1559,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "ENST00000402629.1",
          "protein_id": "ENSP00000386094.1",
          "transcript_support_level": 1,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 359,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 1080,
          "cdna_start": 744,
          "cdna_end": null,
          "cdna_length": 1450,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "n.710T>C",
          "hgvs_p": null,
          "transcript": "ENST00000489769.1",
          "protein_id": "ENSP00000451525.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1562,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "NM_001002235.3",
          "protein_id": "NP_001002235.1",
          "transcript_support_level": null,
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          "aa_length": 418,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 893,
          "cdna_end": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
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        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 5,
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          "exon_count": 7,
          "intron_rank": null,
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          "gene_symbol": "SERPINA1",
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          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "NM_001002236.3",
          "protein_id": "NP_001002236.1",
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          "aa_length": 418,
          "cds_start": 710,
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          "cdna_start": 1207,
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          "cdna_length": 3456,
          "mane_select": null,
          "mane_plus": null,
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          "feature": null
        },
        {
          "aa_ref": "V",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "NM_001127700.2",
          "protein_id": "NP_001121172.1",
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          "aa_start": 237,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 710,
          "cds_end": null,
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          "cdna_start": 930,
          "cdna_end": null,
          "cdna_length": 3179,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
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          "protein_coding": true,
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          ],
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          "intron_rank": null,
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          "cds_start": 710,
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          "mane_select": null,
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        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "NM_001127702.2",
          "protein_id": "NP_001121174.1",
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          "aa_end": null,
          "aa_length": 418,
          "cds_start": 710,
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          "cdna_start": 1034,
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          "cdna_length": 3283,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "NM_001127703.2",
          "protein_id": "NP_001121175.1",
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          "cdna_start": 1189,
          "cdna_end": null,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
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          "aa_alt": "A",
          "canonical": false,
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          "strand": false,
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          ],
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          "intron_rank": null,
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          "gene_symbol": "SERPINA1",
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          "hgvs_c": "c.710T>C",
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          "transcript": "NM_001127704.2",
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        },
        {
          "aa_ref": "V",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
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          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "NM_001127705.2",
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          "cdna_start": 1204,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "NM_001127706.2",
          "protein_id": "NP_001121178.1",
          "transcript_support_level": null,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 997,
          "cdna_end": null,
          "cdna_length": 3246,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SERPINA1",
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          "hgvs_c": "c.710T>C",
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          "transcript_support_level": null,
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          "cdna_length": 3243,
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        {
          "aa_ref": "V",
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          "protein_coding": true,
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          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "SERPINA1",
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          "hgvs_p": "p.Val237Ala",
          "transcript": "ENST00000636712.1",
          "protein_id": "ENSP00000490054.1",
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        },
        {
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          "strand": false,
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          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "SERPINA1",
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          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "XM_017021370.2",
          "protein_id": "XP_016876859.1",
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          "cdna_start": 1132,
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          "cdna_length": 3381,
          "mane_select": null,
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        },
        {
          "aa_ref": "V",
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          "protein_coding": true,
          "strand": false,
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            "missense_variant"
          ],
          "exon_rank": 4,
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          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "XM_047431478.1",
          "protein_id": "XP_047287434.1",
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          "cdna_start": 1031,
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          "cdna_length": 3280,
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        },
        {
          "aa_ref": "V",
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          "protein_coding": true,
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            "missense_variant"
          ],
          "exon_rank": 4,
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          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "SERPINA1",
          "gene_hgnc_id": 8941,
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala",
          "transcript": "XM_047431479.1",
          "protein_id": "XP_047287435.1",
          "transcript_support_level": null,
          "aa_start": 237,
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          "cds_start": 710,
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          "cds_length": 1257,
          "cdna_start": 1135,
          "cdna_end": null,
          "cdna_length": 3384,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "SERPINA1",
      "gene_hgnc_id": 8941,
      "dbsnp": "rs6647",
      "frequency_reference_population": 0.22378713,
      "hom_count_reference_population": 47211,
      "allele_count_reference_population": 360129,
      "gnomad_exomes_af": 0.216065,
      "gnomad_genomes_af": 0.298228,
      "gnomad_exomes_ac": 315025,
      "gnomad_genomes_ac": 45104,
      "gnomad_exomes_homalt": 38288,
      "gnomad_genomes_homalt": 8923,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.000017623495295993052,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.11999999731779099,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.255,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0645,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.44,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.183,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.12,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BA1",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000393087.9",
          "gene_symbol": "SERPINA1",
          "hgnc_id": 8941,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.710T>C",
          "hgvs_p": "p.Val237Ala"
        }
      ],
      "clinvar_disease": " M1A,Alpha-1-antitrypsin deficiency,Inborn genetic diseases,PI,PI M1-ALA213,not provided,not specified",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:1 B:7",
      "phenotype_combined": "PI, M1A|PI M1-ALA213|not specified|Alpha-1-antitrypsin deficiency|not provided|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}