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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-99897194-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=99897194&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 99897194,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000262233.11",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.727A>G",
          "hgvs_p": "p.Thr243Ala",
          "transcript": "NM_004434.3",
          "protein_id": "NP_004425.2",
          "transcript_support_level": null,
          "aa_start": 243,
          "aa_end": null,
          "aa_length": 815,
          "cds_start": 727,
          "cds_end": null,
          "cds_length": 2448,
          "cdna_start": 791,
          "cdna_end": null,
          "cdna_length": 4460,
          "mane_select": "ENST00000262233.11",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.727A>G",
          "hgvs_p": "p.Thr243Ala",
          "transcript": "ENST00000262233.11",
          "protein_id": "ENSP00000262233.7",
          "transcript_support_level": 1,
          "aa_start": 243,
          "aa_end": null,
          "aa_length": 815,
          "cds_start": 727,
          "cds_end": null,
          "cds_length": 2448,
          "cdna_start": 791,
          "cdna_end": null,
          "cdna_length": 4460,
          "mane_select": "NM_004434.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.688A>G",
          "hgvs_p": "p.Thr230Ala",
          "transcript": "ENST00000554479.5",
          "protein_id": "ENSP00000451346.1",
          "transcript_support_level": 1,
          "aa_start": 230,
          "aa_end": null,
          "aa_length": 378,
          "cds_start": 688,
          "cds_end": null,
          "cds_length": 1137,
          "cdna_start": 828,
          "cdna_end": null,
          "cdna_length": 1277,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.802A>G",
          "hgvs_p": "p.Thr268Ala",
          "transcript": "ENST00000649352.1",
          "protein_id": "ENSP00000498100.1",
          "transcript_support_level": null,
          "aa_start": 268,
          "aa_end": null,
          "aa_length": 840,
          "cds_start": 802,
          "cds_end": null,
          "cds_length": 2523,
          "cdna_start": 1181,
          "cdna_end": null,
          "cdna_length": 4804,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.784A>G",
          "hgvs_p": "p.Thr262Ala",
          "transcript": "NM_001008707.2",
          "protein_id": "NP_001008707.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 834,
          "cds_start": 784,
          "cds_end": null,
          "cds_length": 2505,
          "cdna_start": 848,
          "cdna_end": null,
          "cdna_length": 4517,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.784A>G",
          "hgvs_p": "p.Thr262Ala",
          "transcript": "ENST00000334192.8",
          "protein_id": "ENSP00000334314.4",
          "transcript_support_level": 5,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 834,
          "cds_start": 784,
          "cds_end": null,
          "cds_length": 2505,
          "cdna_start": 918,
          "cdna_end": null,
          "cdna_length": 4064,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.745A>G",
          "hgvs_p": "p.Thr249Ala",
          "transcript": "NM_001440375.1",
          "protein_id": "NP_001427304.1",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 821,
          "cds_start": 745,
          "cds_end": null,
          "cds_length": 2466,
          "cdna_start": 941,
          "cdna_end": null,
          "cdna_length": 4610,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.691A>G",
          "hgvs_p": "p.Thr231Ala",
          "transcript": "ENST00000327921.13",
          "protein_id": "ENSP00000327384.9",
          "transcript_support_level": 2,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 803,
          "cds_start": 691,
          "cds_end": null,
          "cds_length": 2412,
          "cdna_start": 1782,
          "cdna_end": null,
          "cdna_length": 3715,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.688A>G",
          "hgvs_p": "p.Thr230Ala",
          "transcript": "NM_001375411.1",
          "protein_id": "NP_001362340.1",
          "transcript_support_level": null,
          "aa_start": 230,
          "aa_end": null,
          "aa_length": 802,
          "cds_start": 688,
          "cds_end": null,
          "cds_length": 2409,
          "cdna_start": 799,
          "cdna_end": null,
          "cdna_length": 4468,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.745A>G",
          "hgvs_p": "p.Thr249Ala",
          "transcript": "NM_001440376.1",
          "protein_id": "NP_001427305.1",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 777,
          "cds_start": 745,
          "cds_end": null,
          "cds_length": 2334,
          "cdna_start": 941,
          "cdna_end": null,
          "cdna_length": 4478,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.727A>G",
          "hgvs_p": "p.Thr243Ala",
          "transcript": "NM_001375412.1",
          "protein_id": "NP_001362341.1",
          "transcript_support_level": null,
          "aa_start": 243,
          "aa_end": null,
          "aa_length": 771,
          "cds_start": 727,
          "cds_end": null,
          "cds_length": 2316,
          "cdna_start": 791,
          "cdna_end": null,
          "cdna_length": 4328,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.688A>G",
          "hgvs_p": "p.Thr230Ala",
          "transcript": "NM_001440377.1",
          "protein_id": "NP_001427306.1",
          "transcript_support_level": null,
          "aa_start": 230,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 688,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": 799,
          "cdna_end": null,
          "cdna_length": 4336,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.634A>G",
          "hgvs_p": "p.Thr212Ala",
          "transcript": "ENST00000556714.5",
          "protein_id": "ENSP00000452089.1",
          "transcript_support_level": 5,
          "aa_start": 212,
          "aa_end": null,
          "aa_length": 284,
          "cds_start": 634,
          "cds_end": null,
          "cds_length": 855,
          "cdna_start": 743,
          "cdna_end": null,
          "cdna_length": 964,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.802A>G",
          "hgvs_p": "p.Thr268Ala",
          "transcript": "XM_005267399.4",
          "protein_id": "XP_005267456.2",
          "transcript_support_level": null,
          "aa_start": 268,
          "aa_end": null,
          "aa_length": 840,
          "cds_start": 802,
          "cds_end": null,
          "cds_length": 2523,
          "cdna_start": 895,
          "cdna_end": null,
          "cdna_length": 4564,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.784A>G",
          "hgvs_p": "p.Thr262Ala",
          "transcript": "XM_005267397.2",
          "protein_id": "XP_005267454.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 834,
          "cds_start": 784,
          "cds_end": null,
          "cds_length": 2505,
          "cdna_start": 848,
          "cdna_end": null,
          "cdna_length": 4517,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.745A>G",
          "hgvs_p": "p.Thr249Ala",
          "transcript": "XM_005267398.3",
          "protein_id": "XP_005267455.1",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 821,
          "cds_start": 745,
          "cds_end": null,
          "cds_length": 2466,
          "cdna_start": 856,
          "cdna_end": null,
          "cdna_length": 4525,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.802A>G",
          "hgvs_p": "p.Thr268Ala",
          "transcript": "XM_011536542.4",
          "protein_id": "XP_011534844.1",
          "transcript_support_level": null,
          "aa_start": 268,
          "aa_end": null,
          "aa_length": 796,
          "cds_start": 802,
          "cds_end": null,
          "cds_length": 2391,
          "cdna_start": 895,
          "cdna_end": null,
          "cdna_length": 4432,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.163A>G",
          "hgvs_p": "p.Thr55Ala",
          "transcript": "XM_024449507.1",
          "protein_id": "XP_024305275.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 627,
          "cds_start": 163,
          "cds_end": null,
          "cds_length": 1884,
          "cdna_start": 456,
          "cdna_end": null,
          "cdna_length": 4125,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "n.356A>G",
          "hgvs_p": null,
          "transcript": "ENST00000555277.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 580,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "n.-48A>G",
          "hgvs_p": null,
          "transcript": "ENST00000554553.5",
          "protein_id": "ENSP00000451706.1",
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 582,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "EML1",
      "gene_hgnc_id": 3330,
      "dbsnp": "rs886037936",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6720061302185059,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.597,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.68,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.23,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.187,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP5_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP5_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000262233.11",
          "gene_symbol": "EML1",
          "hgnc_id": 3330,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.727A>G",
          "hgvs_p": "p.Thr243Ala"
        }
      ],
      "clinvar_disease": "Band heterotopia of brain,not provided",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "phenotype_combined": "Band heterotopia of brain|not provided",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}