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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-99914251-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=99914251&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 99914251,
      "ref": "C",
      "alt": "T",
      "effect": "stop_gained",
      "transcript": "NM_001008707.2",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1567C>T",
          "hgvs_p": "p.Arg523*",
          "transcript": "NM_004434.3",
          "protein_id": "NP_004425.2",
          "transcript_support_level": null,
          "aa_start": 523,
          "aa_end": null,
          "aa_length": 815,
          "cds_start": 1567,
          "cds_end": null,
          "cds_length": 2448,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000262233.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004434.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1567C>T",
          "hgvs_p": "p.Arg523*",
          "transcript": "ENST00000262233.11",
          "protein_id": "ENSP00000262233.7",
          "transcript_support_level": 1,
          "aa_start": 523,
          "aa_end": null,
          "aa_length": 815,
          "cds_start": 1567,
          "cds_end": null,
          "cds_length": 2448,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_004434.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000262233.11"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1741C>T",
          "hgvs_p": "p.Arg581*",
          "transcript": "ENST00000909081.1",
          "protein_id": "ENSP00000579140.1",
          "transcript_support_level": null,
          "aa_start": 581,
          "aa_end": null,
          "aa_length": 873,
          "cds_start": 1741,
          "cds_end": null,
          "cds_length": 2622,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909081.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1567C>T",
          "hgvs_p": "p.Arg523*",
          "transcript": "ENST00000940980.1",
          "protein_id": "ENSP00000611039.1",
          "transcript_support_level": null,
          "aa_start": 523,
          "aa_end": null,
          "aa_length": 846,
          "cds_start": 1567,
          "cds_end": null,
          "cds_length": 2541,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940980.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1642C>T",
          "hgvs_p": "p.Arg548*",
          "transcript": "ENST00000649352.1",
          "protein_id": "ENSP00000498100.1",
          "transcript_support_level": null,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 840,
          "cds_start": 1642,
          "cds_end": null,
          "cds_length": 2523,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000649352.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1624C>T",
          "hgvs_p": "p.Arg542*",
          "transcript": "NM_001008707.2",
          "protein_id": "NP_001008707.1",
          "transcript_support_level": null,
          "aa_start": 542,
          "aa_end": null,
          "aa_length": 834,
          "cds_start": 1624,
          "cds_end": null,
          "cds_length": 2505,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001008707.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1624C>T",
          "hgvs_p": "p.Arg542*",
          "transcript": "ENST00000334192.8",
          "protein_id": "ENSP00000334314.4",
          "transcript_support_level": 5,
          "aa_start": 542,
          "aa_end": null,
          "aa_length": 834,
          "cds_start": 1624,
          "cds_end": null,
          "cds_length": 2505,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000334192.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1594C>T",
          "hgvs_p": "p.Arg532*",
          "transcript": "ENST00000909078.1",
          "protein_id": "ENSP00000579137.1",
          "transcript_support_level": null,
          "aa_start": 532,
          "aa_end": null,
          "aa_length": 824,
          "cds_start": 1594,
          "cds_end": null,
          "cds_length": 2475,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909078.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1585C>T",
          "hgvs_p": "p.Arg529*",
          "transcript": "NM_001440375.1",
          "protein_id": "NP_001427304.1",
          "transcript_support_level": null,
          "aa_start": 529,
          "aa_end": null,
          "aa_length": 821,
          "cds_start": 1585,
          "cds_end": null,
          "cds_length": 2466,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001440375.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1564C>T",
          "hgvs_p": "p.Arg522*",
          "transcript": "ENST00000940979.1",
          "protein_id": "ENSP00000611038.1",
          "transcript_support_level": null,
          "aa_start": 522,
          "aa_end": null,
          "aa_length": 814,
          "cds_start": 1564,
          "cds_end": null,
          "cds_length": 2445,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940979.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1531C>T",
          "hgvs_p": "p.Arg511*",
          "transcript": "ENST00000327921.13",
          "protein_id": "ENSP00000327384.9",
          "transcript_support_level": 2,
          "aa_start": 511,
          "aa_end": null,
          "aa_length": 803,
          "cds_start": 1531,
          "cds_end": null,
          "cds_length": 2412,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000327921.13"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1528C>T",
          "hgvs_p": "p.Arg510*",
          "transcript": "NM_001375411.1",
          "protein_id": "NP_001362340.1",
          "transcript_support_level": null,
          "aa_start": 510,
          "aa_end": null,
          "aa_length": 802,
          "cds_start": 1528,
          "cds_end": null,
          "cds_length": 2409,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001375411.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1513C>T",
          "hgvs_p": "p.Arg505*",
          "transcript": "ENST00000909079.1",
          "protein_id": "ENSP00000579138.1",
          "transcript_support_level": null,
          "aa_start": 505,
          "aa_end": null,
          "aa_length": 797,
          "cds_start": 1513,
          "cds_end": null,
          "cds_length": 2394,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909079.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1585C>T",
          "hgvs_p": "p.Arg529*",
          "transcript": "NM_001440376.1",
          "protein_id": "NP_001427305.1",
          "transcript_support_level": null,
          "aa_start": 529,
          "aa_end": null,
          "aa_length": 777,
          "cds_start": 1585,
          "cds_end": null,
          "cds_length": 2334,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001440376.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1567C>T",
          "hgvs_p": "p.Arg523*",
          "transcript": "NM_001375412.1",
          "protein_id": "NP_001362341.1",
          "transcript_support_level": null,
          "aa_start": 523,
          "aa_end": null,
          "aa_length": 771,
          "cds_start": 1567,
          "cds_end": null,
          "cds_length": 2316,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001375412.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1567C>T",
          "hgvs_p": "p.Arg523*",
          "transcript": "ENST00000909082.1",
          "protein_id": "ENSP00000579141.1",
          "transcript_support_level": null,
          "aa_start": 523,
          "aa_end": null,
          "aa_length": 771,
          "cds_start": 1567,
          "cds_end": null,
          "cds_length": 2316,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909082.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1528C>T",
          "hgvs_p": "p.Arg510*",
          "transcript": "NM_001440377.1",
          "protein_id": "NP_001427306.1",
          "transcript_support_level": null,
          "aa_start": 510,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 1528,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001440377.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1384C>T",
          "hgvs_p": "p.Arg462*",
          "transcript": "ENST00000909080.1",
          "protein_id": "ENSP00000579139.1",
          "transcript_support_level": null,
          "aa_start": 462,
          "aa_end": null,
          "aa_length": 754,
          "cds_start": 1384,
          "cds_end": null,
          "cds_length": 2265,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909080.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1642C>T",
          "hgvs_p": "p.Arg548*",
          "transcript": "XM_005267399.4",
          "protein_id": "XP_005267456.2",
          "transcript_support_level": null,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 840,
          "cds_start": 1642,
          "cds_end": null,
          "cds_length": 2523,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005267399.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML1",
          "gene_hgnc_id": 3330,
          "hgvs_c": "c.1624C>T",
          "hgvs_p": "p.Arg542*",
          "transcript": "XM_005267397.2",
          "protein_id": "XP_005267454.1",
          "transcript_support_level": null,
          "aa_start": 542,
          "aa_end": null,
          "aa_length": 834,
          "cds_start": 1624,
          "cds_end": null,
          "cds_length": 2505,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        {
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          "biotype": "retained_intron",
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      ],
      "gene_symbol": "EML1",
      "gene_hgnc_id": 3330,
      "dbsnp": "rs1555404109",
      "frequency_reference_population": 6.8411805e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84118e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5600000023841858,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.56,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 2.107,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 12,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Moderate",
      "acmg_by_gene": [
        {
          "score": 12,
          "benign_score": 0,
          "pathogenic_score": 12,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5_Moderate"
          ],
          "verdict": "Pathogenic",
          "transcript": "NM_001008707.2",
          "gene_symbol": "EML1",
          "hgnc_id": 3330,
          "effects": [
            "stop_gained"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1624C>T",
          "hgvs_p": "p.Arg542*"
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      ],
      "clinvar_disease": "Band heterotopia of brain",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "P:1",
      "phenotype_combined": "Band heterotopia of brain",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}