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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 15-26772403-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=15&pos=26772403&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "15",
      "pos": 26772403,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "ENST00000311550.10",
      "consequences": [
        {
          "aa_ref": "M",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "c.239T>G",
          "hgvs_p": "p.Met80Arg",
          "transcript": "NM_000814.6",
          "protein_id": "NP_000805.1",
          "transcript_support_level": null,
          "aa_start": 80,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": 239,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": 343,
          "cdna_end": null,
          "cdna_length": 5767,
          "mane_select": "ENST00000311550.10",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "c.239T>G",
          "hgvs_p": "p.Met80Arg",
          "transcript": "ENST00000311550.10",
          "protein_id": "ENSP00000308725.5",
          "transcript_support_level": 1,
          "aa_start": 80,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": 239,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": 343,
          "cdna_end": null,
          "cdna_length": 5767,
          "mane_select": "NM_000814.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "c.407T>G",
          "hgvs_p": "p.Met136Arg",
          "transcript": "ENST00000541819.6",
          "protein_id": "ENSP00000442408.2",
          "transcript_support_level": 1,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 529,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 1590,
          "cdna_start": 510,
          "cdna_end": null,
          "cdna_length": 5934,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "c.239T>G",
          "hgvs_p": "p.Met80Arg",
          "transcript": "ENST00000299267.9",
          "protein_id": "ENSP00000299267.4",
          "transcript_support_level": 1,
          "aa_start": 80,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": 239,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": 278,
          "cdna_end": null,
          "cdna_length": 5702,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "c.239T>G",
          "hgvs_p": "p.Met80Arg",
          "transcript": "NM_021912.5",
          "protein_id": "NP_068712.1",
          "transcript_support_level": null,
          "aa_start": 80,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": 239,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": 278,
          "cdna_end": null,
          "cdna_length": 5702,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "c.140T>G",
          "hgvs_p": "p.Met47Arg",
          "transcript": "ENST00000638099.1",
          "protein_id": "ENSP00000490678.1",
          "transcript_support_level": 5,
          "aa_start": 47,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 140,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": 220,
          "cdna_end": null,
          "cdna_length": 2752,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "c.-113T>G",
          "hgvs_p": null,
          "transcript": "NM_001278631.2",
          "protein_id": "NP_001265560.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 388,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1167,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5863,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "n.239T>G",
          "hgvs_p": null,
          "transcript": "ENST00000554556.5",
          "protein_id": "ENSP00000451077.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 817,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "n.266T>G",
          "hgvs_p": null,
          "transcript": "ENST00000554722.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 406,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "n.239T>G",
          "hgvs_p": null,
          "transcript": "ENST00000555632.5",
          "protein_id": "ENSP00000452041.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2109,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "n.611T>G",
          "hgvs_p": null,
          "transcript": "ENST00000557641.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 828,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "c.-113T>G",
          "hgvs_p": null,
          "transcript": "NM_001278631.2",
          "protein_id": "NP_001265560.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 388,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1167,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5863,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GABRB3",
          "gene_hgnc_id": 4083,
          "hgvs_c": "c.-420T>G",
          "hgvs_p": null,
          "transcript": "ENST00000636466.1",
          "protein_id": "ENSP00000489768.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 388,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1167,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4509,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "GABRB3",
      "gene_hgnc_id": 4083,
      "dbsnp": "rs1064794797",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9516788125038147,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.7300000190734863,
      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.941,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9751,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.34,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 5.089,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.874147833448651,
      "dbscsnv_ada_prediction": "Benign",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 11,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM1,PM2,PM5,PP3_Strong,PP5",
      "acmg_by_gene": [
        {
          "score": 11,
          "benign_score": 0,
          "pathogenic_score": 11,
          "criteria": [
            "PM1",
            "PM2",
            "PM5",
            "PP3_Strong",
            "PP5"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000311550.10",
          "gene_symbol": "GABRB3",
          "hgnc_id": 4083,
          "effects": [
            "missense_variant",
            "splice_region_variant"
          ],
          "inheritance_mode": "AD,Unknown",
          "hgvs_c": "c.239T>G",
          "hgvs_p": "p.Met80Arg"
        }
      ],
      "clinvar_disease": " 1, 43, 5, childhood absence, susceptibility to,Developmental and epileptic encephalopathy,Epilepsy,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "P:1 US:1",
      "phenotype_combined": "Developmental and epileptic encephalopathy, 43|Epilepsy, childhood absence, susceptibility to, 1;Epilepsy, childhood absence, susceptibility to, 5|not provided",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}