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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 15-40217523-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=15&pos=40217523&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "15",
      "pos": 40217523,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001211.6",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B",
          "gene_hgnc_id": 1149,
          "hgvs_c": "c.2706G>T",
          "hgvs_p": "p.Lys902Asn",
          "transcript": "NM_001211.6",
          "protein_id": "NP_001202.5",
          "transcript_support_level": null,
          "aa_start": 902,
          "aa_end": null,
          "aa_length": 1050,
          "cds_start": 2706,
          "cds_end": null,
          "cds_length": 3153,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000287598.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001211.6"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B",
          "gene_hgnc_id": 1149,
          "hgvs_c": "c.2706G>T",
          "hgvs_p": "p.Lys902Asn",
          "transcript": "ENST00000287598.11",
          "protein_id": "ENSP00000287598.7",
          "transcript_support_level": 1,
          "aa_start": 902,
          "aa_end": null,
          "aa_length": 1050,
          "cds_start": 2706,
          "cds_end": null,
          "cds_length": 3153,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001211.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000287598.11"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B",
          "gene_hgnc_id": 1149,
          "hgvs_c": "c.2748G>T",
          "hgvs_p": "p.Lys916Asn",
          "transcript": "ENST00000412359.7",
          "protein_id": "ENSP00000398470.3",
          "transcript_support_level": 2,
          "aa_start": 916,
          "aa_end": null,
          "aa_length": 1064,
          "cds_start": 2748,
          "cds_end": null,
          "cds_length": 3195,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000412359.7"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B",
          "gene_hgnc_id": 1149,
          "hgvs_c": "c.2808G>T",
          "hgvs_p": "p.Lys936Asn",
          "transcript": "ENST00000918306.1",
          "protein_id": "ENSP00000588365.1",
          "transcript_support_level": null,
          "aa_start": 936,
          "aa_end": null,
          "aa_length": 1084,
          "cds_start": 2808,
          "cds_end": null,
          "cds_length": 3255,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918306.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B",
          "gene_hgnc_id": 1149,
          "hgvs_c": "c.2724G>T",
          "hgvs_p": "p.Lys908Asn",
          "transcript": "ENST00000918309.1",
          "protein_id": "ENSP00000588368.1",
          "transcript_support_level": null,
          "aa_start": 908,
          "aa_end": null,
          "aa_length": 1056,
          "cds_start": 2724,
          "cds_end": null,
          "cds_length": 3171,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918309.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B",
          "gene_hgnc_id": 1149,
          "hgvs_c": "c.2721G>T",
          "hgvs_p": "p.Lys907Asn",
          "transcript": "ENST00000897791.1",
          "protein_id": "ENSP00000567850.1",
          "transcript_support_level": null,
          "aa_start": 907,
          "aa_end": null,
          "aa_length": 1055,
          "cds_start": 2721,
          "cds_end": null,
          "cds_length": 3168,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897791.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B",
          "gene_hgnc_id": 1149,
          "hgvs_c": "c.2661G>T",
          "hgvs_p": "p.Lys887Asn",
          "transcript": "ENST00000897792.1",
          "protein_id": "ENSP00000567851.1",
          "transcript_support_level": null,
          "aa_start": 887,
          "aa_end": null,
          "aa_length": 1035,
          "cds_start": 2661,
          "cds_end": null,
          "cds_length": 3108,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897792.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B",
          "gene_hgnc_id": 1149,
          "hgvs_c": "c.2646G>T",
          "hgvs_p": "p.Lys882Asn",
          "transcript": "ENST00000918305.1",
          "protein_id": "ENSP00000588364.1",
          "transcript_support_level": null,
          "aa_start": 882,
          "aa_end": null,
          "aa_length": 1030,
          "cds_start": 2646,
          "cds_end": null,
          "cds_length": 3093,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918305.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B",
          "gene_hgnc_id": 1149,
          "hgvs_c": "c.2304G>T",
          "hgvs_p": "p.Lys768Asn",
          "transcript": "ENST00000918310.1",
          "protein_id": "ENSP00000588369.1",
          "transcript_support_level": null,
          "aa_start": 768,
          "aa_end": null,
          "aa_length": 916,
          "cds_start": 2304,
          "cds_end": null,
          "cds_length": 2751,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918310.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B",
          "gene_hgnc_id": 1149,
          "hgvs_c": "c.2229G>T",
          "hgvs_p": "p.Lys743Asn",
          "transcript": "ENST00000918308.1",
          "protein_id": "ENSP00000588367.1",
          "transcript_support_level": null,
          "aa_start": 743,
          "aa_end": null,
          "aa_length": 891,
          "cds_start": 2229,
          "cds_end": null,
          "cds_length": 2676,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918308.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B",
          "gene_hgnc_id": 1149,
          "hgvs_c": "c.2160G>T",
          "hgvs_p": "p.Lys720Asn",
          "transcript": "ENST00000918307.1",
          "protein_id": "ENSP00000588366.1",
          "transcript_support_level": null,
          "aa_start": 720,
          "aa_end": null,
          "aa_length": 868,
          "cds_start": 2160,
          "cds_end": null,
          "cds_length": 2607,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000918307.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B-PAK6",
          "gene_hgnc_id": 52276,
          "hgvs_c": "c.-345G>T",
          "hgvs_p": null,
          "transcript": "NM_001128628.3",
          "protein_id": "NP_001122100.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 681,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2046,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001128628.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B-PAK6",
          "gene_hgnc_id": 52276,
          "hgvs_c": "c.-262G>T",
          "hgvs_p": null,
          "transcript": "NM_001128629.3",
          "protein_id": "NP_001122101.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 681,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2046,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001128629.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAK6",
          "gene_hgnc_id": 16061,
          "hgvs_c": "c.-345G>T",
          "hgvs_p": null,
          "transcript": "ENST00000441369.6",
          "protein_id": "ENSP00000406873.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 681,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2046,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000441369.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAK6",
          "gene_hgnc_id": 16061,
          "hgvs_c": "c.-262G>T",
          "hgvs_p": null,
          "transcript": "ENST00000453867.7",
          "protein_id": "ENSP00000401153.3",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 681,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2046,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000453867.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BUB1B",
          "gene_hgnc_id": 1149,
          "hgvs_c": "n.107G>T",
          "hgvs_p": null,
          "transcript": "ENST00000558151.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000558151.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "LOC107984763",
          "gene_hgnc_id": null,
          "hgvs_c": "n.217+21962C>A",
          "hgvs_p": null,
          "transcript": "XR_001751506.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_001751506.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAK6",
          "gene_hgnc_id": 16061,
          "hgvs_c": "c.-345G>T",
          "hgvs_p": null,
          "transcript": "ENST00000558658.6",
          "protein_id": "ENSP00000456785.2",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 681,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2046,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000558658.6"
        }
      ],
      "gene_symbol": "BUB1B",
      "gene_hgnc_id": 1149,
      "dbsnp": "rs1595538961",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.14010551571846008,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.25999999046325684,
      "splice_prediction_selected": "Uncertain_significance",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.034,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2208,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.69,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.979,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.26,
      "spliceai_max_prediction": "Uncertain_significance",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001211.6",
          "gene_symbol": "BUB1B",
          "hgnc_id": 1149,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.2706G>T",
          "hgvs_p": "p.Lys902Asn"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001128628.3",
          "gene_symbol": "BUB1B-PAK6",
          "hgnc_id": 52276,
          "effects": [
            "5_prime_UTR_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.-345G>T",
          "hgvs_p": null
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000441369.6",
          "gene_symbol": "PAK6",
          "hgnc_id": 16061,
          "effects": [
            "5_prime_UTR_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-345G>T",
          "hgvs_p": null
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "XR_001751506.2",
          "gene_symbol": "LOC107984763",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.217+21962C>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Inborn genetic diseases",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}