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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 15-42410633-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=15&pos=42410633&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "15",
      "pos": 42410633,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000397163.8",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.2230A>G",
          "hgvs_p": "p.Ser744Gly",
          "transcript": "NM_000070.3",
          "protein_id": "NP_000061.1",
          "transcript_support_level": null,
          "aa_start": 744,
          "aa_end": null,
          "aa_length": 821,
          "cds_start": 2230,
          "cds_end": null,
          "cds_length": 2466,
          "cdna_start": 2535,
          "cdna_end": null,
          "cdna_length": 3315,
          "mane_select": "ENST00000397163.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.2230A>G",
          "hgvs_p": "p.Ser744Gly",
          "transcript": "ENST00000397163.8",
          "protein_id": "ENSP00000380349.3",
          "transcript_support_level": 1,
          "aa_start": 744,
          "aa_end": null,
          "aa_length": 821,
          "cds_start": 2230,
          "cds_end": null,
          "cds_length": 2466,
          "cdna_start": 2535,
          "cdna_end": null,
          "cdna_length": 3315,
          "mane_select": "NM_000070.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.2212A>G",
          "hgvs_p": "p.Ser738Gly",
          "transcript": "ENST00000357568.8",
          "protein_id": "ENSP00000350181.3",
          "transcript_support_level": 1,
          "aa_start": 738,
          "aa_end": null,
          "aa_length": 815,
          "cds_start": 2212,
          "cds_end": null,
          "cds_length": 2448,
          "cdna_start": 2433,
          "cdna_end": null,
          "cdna_length": 3130,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.1954A>G",
          "hgvs_p": "p.Ser652Gly",
          "transcript": "ENST00000349748.8",
          "protein_id": "ENSP00000183936.4",
          "transcript_support_level": 1,
          "aa_start": 652,
          "aa_end": null,
          "aa_length": 729,
          "cds_start": 1954,
          "cds_end": null,
          "cds_length": 2190,
          "cdna_start": 2262,
          "cdna_end": null,
          "cdna_length": 3045,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.694A>G",
          "hgvs_p": "p.Ser232Gly",
          "transcript": "ENST00000397200.8",
          "protein_id": "ENSP00000380384.4",
          "transcript_support_level": 1,
          "aa_start": 232,
          "aa_end": null,
          "aa_length": 309,
          "cds_start": 694,
          "cds_end": null,
          "cds_length": 930,
          "cdna_start": 863,
          "cdna_end": null,
          "cdna_length": 1643,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.235A>G",
          "hgvs_p": "p.Ser79Gly",
          "transcript": "ENST00000561817.5",
          "protein_id": "ENSP00000456575.1",
          "transcript_support_level": 1,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 191,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 576,
          "cdna_start": 474,
          "cdna_end": null,
          "cdna_length": 915,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.235A>G",
          "hgvs_p": "p.Ser79Gly",
          "transcript": "ENST00000337571.9",
          "protein_id": "ENSP00000336840.4",
          "transcript_support_level": 1,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 156,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 471,
          "cdna_start": 524,
          "cdna_end": null,
          "cdna_length": 1304,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.235A>G",
          "hgvs_p": "p.Ser79Gly",
          "transcript": "ENST00000397204.9",
          "protein_id": "ENSP00000380387.4",
          "transcript_support_level": 1,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 156,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 471,
          "cdna_start": 485,
          "cdna_end": null,
          "cdna_length": 1263,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.235A>G",
          "hgvs_p": "p.Ser79Gly",
          "transcript": "ENST00000673886.1",
          "protein_id": "ENSP00000501155.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 156,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 471,
          "cdna_start": 1313,
          "cdna_end": null,
          "cdna_length": 2078,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.235A>G",
          "hgvs_p": "p.Ser79Gly",
          "transcript": "ENST00000673928.1",
          "protein_id": "ENSP00000501099.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 156,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 471,
          "cdna_start": 676,
          "cdna_end": null,
          "cdna_length": 1437,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.235A>G",
          "hgvs_p": "p.Ser79Gly",
          "transcript": "ENST00000674146.1",
          "protein_id": "ENSP00000501175.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 156,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 471,
          "cdna_start": 968,
          "cdna_end": null,
          "cdna_length": 1733,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.235A>G",
          "hgvs_p": "p.Ser79Gly",
          "transcript": "ENST00000674149.1",
          "protein_id": "ENSP00000501112.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 156,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 471,
          "cdna_start": 954,
          "cdna_end": null,
          "cdna_length": 1707,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.133A>G",
          "hgvs_p": "p.Ser45Gly",
          "transcript": "ENST00000673743.1",
          "protein_id": "ENSP00000500989.1",
          "transcript_support_level": null,
          "aa_start": 45,
          "aa_end": null,
          "aa_length": 122,
          "cds_start": 133,
          "cds_end": null,
          "cds_length": 369,
          "cdna_start": 761,
          "cdna_end": null,
          "cdna_length": 1526,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000258461",
          "gene_hgnc_id": null,
          "hgvs_c": "n.*2666A>G",
          "hgvs_p": null,
          "transcript": "ENST00000495723.1",
          "protein_id": "ENSP00000492063.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3881,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000258461",
          "gene_hgnc_id": null,
          "hgvs_c": "n.*2666A>G",
          "hgvs_p": null,
          "transcript": "ENST00000495723.1",
          "protein_id": "ENSP00000492063.1",
          "transcript_support_level": 2,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3881,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.2212A>G",
          "hgvs_p": "p.Ser738Gly",
          "transcript": "NM_024344.2",
          "protein_id": "NP_077320.1",
          "transcript_support_level": null,
          "aa_start": 738,
          "aa_end": null,
          "aa_length": 815,
          "cds_start": 2212,
          "cds_end": null,
          "cds_length": 2448,
          "cdna_start": 2517,
          "cdna_end": null,
          "cdna_length": 3297,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.2086A>G",
          "hgvs_p": "p.Ser696Gly",
          "transcript": "ENST00000318023.11",
          "protein_id": "ENSP00000326281.8",
          "transcript_support_level": 5,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 773,
          "cds_start": 2086,
          "cds_end": null,
          "cds_length": 2322,
          "cdna_start": 2392,
          "cdna_end": null,
          "cdna_length": 3172,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.1954A>G",
          "hgvs_p": "p.Ser652Gly",
          "transcript": "NM_173087.2",
          "protein_id": "NP_775110.1",
          "transcript_support_level": null,
          "aa_start": 652,
          "aa_end": null,
          "aa_length": 729,
          "cds_start": 1954,
          "cds_end": null,
          "cds_length": 2190,
          "cdna_start": 2259,
          "cdna_end": null,
          "cdna_length": 3039,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.694A>G",
          "hgvs_p": "p.Ser232Gly",
          "transcript": "NM_173088.2",
          "protein_id": "NP_775111.1",
          "transcript_support_level": null,
          "aa_start": 232,
          "aa_end": null,
          "aa_length": 309,
          "cds_start": 694,
          "cds_end": null,
          "cds_length": 930,
          "cdna_start": 886,
          "cdna_end": null,
          "cdna_length": 1666,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.562A>G",
          "hgvs_p": "p.Ser188Gly",
          "transcript": "ENST00000569827.5",
          "protein_id": "ENSP00000454379.1",
          "transcript_support_level": 5,
          "aa_start": 188,
          "aa_end": null,
          "aa_length": 254,
          "cds_start": 562,
          "cds_end": null,
          "cds_length": 767,
          "cdna_start": 703,
          "cdna_end": null,
          "cdna_length": 908,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAPN3",
          "gene_hgnc_id": 1480,
          "hgvs_c": "c.454A>G",
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      "gene_symbol": "CAPN3",
      "gene_hgnc_id": 1480,
      "dbsnp": "rs750083132",
      "frequency_reference_population": 0.000002052211,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000205221,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.949825644493103,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.719,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.4187,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.2,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 4.257,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 18,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM1,PM2,PM5,PP3_Strong,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 18,
          "benign_score": 0,
          "pathogenic_score": 18,
          "criteria": [
            "PM1",
            "PM2",
            "PM5",
            "PP3_Strong",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000397163.8",
          "gene_symbol": "CAPN3",
          "hgnc_id": 1480,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.2230A>G",
          "hgvs_p": "p.Ser744Gly"
        },
        {
          "score": 14,
          "benign_score": 0,
          "pathogenic_score": 14,
          "criteria": [
            "PM2",
            "PP3_Strong",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000495723.1",
          "gene_symbol": "ENSG00000258461",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.*2666A>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " autosomal dominant 4, limb-girdle,Autosomal recessive limb-girdle muscular dystrophy,Autosomal recessive limb-girdle muscular dystrophy type 2A,Muscular dystrophy,not provided",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:4 LP:1",
      "phenotype_combined": "Autosomal recessive limb-girdle muscular dystrophy type 2A|not provided|Autosomal recessive limb-girdle muscular dystrophy|Muscular dystrophy, limb-girdle, autosomal dominant 4",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}