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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 15-48470705-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=15&pos=48470705&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "15",
      "pos": 48470705,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_000138.5",
      "consequences": [
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 66,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FBN1",
          "gene_hgnc_id": 3603,
          "hgvs_c": "c.4388A>G",
          "hgvs_p": "p.Asn1463Ser",
          "transcript": "NM_000138.5",
          "protein_id": "NP_000129.3",
          "transcript_support_level": null,
          "aa_start": 1463,
          "aa_end": null,
          "aa_length": 2871,
          "cds_start": 4388,
          "cds_end": null,
          "cds_length": 8616,
          "cdna_start": 4704,
          "cdna_end": null,
          "cdna_length": 11609,
          "mane_select": "ENST00000316623.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000138.5"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 66,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FBN1",
          "gene_hgnc_id": 3603,
          "hgvs_c": "c.4388A>G",
          "hgvs_p": "p.Asn1463Ser",
          "transcript": "ENST00000316623.10",
          "protein_id": "ENSP00000325527.5",
          "transcript_support_level": 1,
          "aa_start": 1463,
          "aa_end": null,
          "aa_length": 2871,
          "cds_start": 4388,
          "cds_end": null,
          "cds_length": 8616,
          "cdna_start": 4704,
          "cdna_end": null,
          "cdna_length": 11609,
          "mane_select": "NM_000138.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000316623.10"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 67,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FBN1",
          "gene_hgnc_id": 3603,
          "hgvs_c": "n.4388A>G",
          "hgvs_p": null,
          "transcript": "ENST00000559133.6",
          "protein_id": "ENSP00000453958.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 9910,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000559133.6"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 35,
          "exon_rank_end": null,
          "exon_count": 65,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FBN1",
          "gene_hgnc_id": 3603,
          "hgvs_c": "c.4388A>G",
          "hgvs_p": "p.Asn1463Ser",
          "transcript": "NM_001406716.1",
          "protein_id": "NP_001393645.1",
          "transcript_support_level": null,
          "aa_start": 1463,
          "aa_end": null,
          "aa_length": 2871,
          "cds_start": 4388,
          "cds_end": null,
          "cds_length": 8616,
          "cdna_start": 4589,
          "cdna_end": null,
          "cdna_length": 11494,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001406716.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FBN1",
          "gene_hgnc_id": 3603,
          "hgvs_c": "n.*151A>G",
          "hgvs_p": null,
          "transcript": "ENST00000537463.6",
          "protein_id": "ENSP00000440294.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3766,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000537463.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 68,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FBN1",
          "gene_hgnc_id": 3603,
          "hgvs_c": "n.4388A>G",
          "hgvs_p": null,
          "transcript": "ENST00000674301.2",
          "protein_id": "ENSP00000501333.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 8995,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000674301.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FBN1",
          "gene_hgnc_id": 3603,
          "hgvs_c": "n.355A>G",
          "hgvs_p": null,
          "transcript": "ENST00000683268.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 967,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000683268.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 38,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FBN1",
          "gene_hgnc_id": 3603,
          "hgvs_c": "n.3062A>G",
          "hgvs_p": null,
          "transcript": "ENST00000684448.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5150,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000684448.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FBN1",
          "gene_hgnc_id": 3603,
          "hgvs_c": "n.*151A>G",
          "hgvs_p": null,
          "transcript": "ENST00000537463.6",
          "protein_id": "ENSP00000440294.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3766,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000537463.6"
        }
      ],
      "gene_symbol": "FBN1",
      "gene_hgnc_id": 3603,
      "dbsnp": "rs1555397413",
      "frequency_reference_population": 6.840535e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84053e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9931122064590454,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.15000000596046448,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.959,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.7999,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.16,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 8.017,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.15,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 12,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM1,PM2,PM5,PP2,PP3_Strong,PP5",
      "acmg_by_gene": [
        {
          "score": 12,
          "benign_score": 0,
          "pathogenic_score": 12,
          "criteria": [
            "PM1",
            "PM2",
            "PM5",
            "PP2",
            "PP3_Strong",
            "PP5"
          ],
          "verdict": "Pathogenic",
          "transcript": "NM_000138.5",
          "gene_symbol": "FBN1",
          "hgnc_id": 3603,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,Unknown,AR",
          "hgvs_c": "c.4388A>G",
          "hgvs_p": "p.Asn1463Ser"
        }
      ],
      "clinvar_disease": "8 conditions,Disproportionate tall stature,Familial thoracic aortic aneurysm and aortic dissection,High palate,Lumbar scoliosis,Mitral valve prolapse,Myxomatous mitral valve degeneration,Progeroid and marfanoid aspect-lipodystrophy syndrome,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "P:2 LP:1 US:2",
      "phenotype_combined": "Mitral valve prolapse;Lumbar scoliosis;Myxomatous mitral valve degeneration;High palate;Disproportionate tall stature|Familial thoracic aortic aneurysm and aortic dissection|Progeroid and marfanoid aspect-lipodystrophy syndrome|not provided|8 conditions",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}
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