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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 15-60531767-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=15&pos=60531767&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "15",
      "pos": 60531767,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_134260.3",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.281A>G",
          "hgvs_p": "p.Lys94Arg",
          "transcript": "NM_134261.3",
          "protein_id": "NP_599023.1",
          "transcript_support_level": null,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 523,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 1572,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000335670.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_134261.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.281A>G",
          "hgvs_p": "p.Lys94Arg",
          "transcript": "ENST00000335670.11",
          "protein_id": "ENSP00000335087.6",
          "transcript_support_level": 1,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 523,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 1572,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_134261.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000335670.11"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.380A>G",
          "hgvs_p": "p.Lys127Arg",
          "transcript": "ENST00000261523.9",
          "protein_id": "ENSP00000261523.5",
          "transcript_support_level": 1,
          "aa_start": 127,
          "aa_end": null,
          "aa_length": 556,
          "cds_start": 380,
          "cds_end": null,
          "cds_length": 1671,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000261523.9"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.356A>G",
          "hgvs_p": "p.Lys119Arg",
          "transcript": "ENST00000309157.8",
          "protein_id": "ENSP00000309753.3",
          "transcript_support_level": 1,
          "aa_start": 119,
          "aa_end": null,
          "aa_length": 548,
          "cds_start": 356,
          "cds_end": null,
          "cds_length": 1647,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000309157.8"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.116A>G",
          "hgvs_p": "p.Lys39Arg",
          "transcript": "ENST00000449337.6",
          "protein_id": "ENSP00000402971.2",
          "transcript_support_level": 1,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 468,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 1407,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000449337.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "n.216A>G",
          "hgvs_p": null,
          "transcript": "ENST00000558234.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000558234.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.380A>G",
          "hgvs_p": "p.Lys127Arg",
          "transcript": "NM_134260.3",
          "protein_id": "NP_599022.1",
          "transcript_support_level": null,
          "aa_start": 127,
          "aa_end": null,
          "aa_length": 556,
          "cds_start": 380,
          "cds_end": null,
          "cds_length": 1671,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_134260.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.356A>G",
          "hgvs_p": "p.Lys119Arg",
          "transcript": "NM_002943.4",
          "protein_id": "NP_002934.1",
          "transcript_support_level": null,
          "aa_start": 119,
          "aa_end": null,
          "aa_length": 548,
          "cds_start": 356,
          "cds_end": null,
          "cds_length": 1647,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002943.4"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.116A>G",
          "hgvs_p": "p.Lys39Arg",
          "transcript": "NM_134262.3",
          "protein_id": "NP_599024.1",
          "transcript_support_level": null,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 468,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 1407,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_134262.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.287A>G",
          "hgvs_p": "p.Lys96Arg",
          "transcript": "ENST00000559343.1",
          "protein_id": "ENSP00000453322.1",
          "transcript_support_level": 4,
          "aa_start": 96,
          "aa_end": null,
          "aa_length": 152,
          "cds_start": 287,
          "cds_end": null,
          "cds_length": 460,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000559343.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.287A>G",
          "hgvs_p": "p.Lys96Arg",
          "transcript": "XM_011521874.2",
          "protein_id": "XP_011520176.1",
          "transcript_support_level": null,
          "aa_start": 96,
          "aa_end": null,
          "aa_length": 525,
          "cds_start": 287,
          "cds_end": null,
          "cds_length": 1578,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011521874.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.224A>G",
          "hgvs_p": "p.Lys75Arg",
          "transcript": "XM_011521875.3",
          "protein_id": "XP_011520177.1",
          "transcript_support_level": null,
          "aa_start": 75,
          "aa_end": null,
          "aa_length": 504,
          "cds_start": 224,
          "cds_end": null,
          "cds_length": 1515,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011521875.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.113A>G",
          "hgvs_p": "p.Lys38Arg",
          "transcript": "XM_011521877.4",
          "protein_id": "XP_011520179.1",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 467,
          "cds_start": 113,
          "cds_end": null,
          "cds_length": 1404,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011521877.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.-131A>G",
          "hgvs_p": null,
          "transcript": "XM_047432928.1",
          "protein_id": "XP_047288884.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 386,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1161,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047432928.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.-131A>G",
          "hgvs_p": null,
          "transcript": "XM_047432929.1",
          "protein_id": "XP_047288885.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 386,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1161,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047432929.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.-131A>G",
          "hgvs_p": null,
          "transcript": "XM_047432930.1",
          "protein_id": "XP_047288886.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 386,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1161,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047432930.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.-131A>G",
          "hgvs_p": null,
          "transcript": "XM_047432928.1",
          "protein_id": "XP_047288884.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 386,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1161,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047432928.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.-131A>G",
          "hgvs_p": null,
          "transcript": "XM_047432929.1",
          "protein_id": "XP_047288885.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 386,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1161,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047432929.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "c.-131A>G",
          "hgvs_p": null,
          "transcript": "XM_047432930.1",
          "protein_id": "XP_047288886.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 386,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1161,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047432930.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RORA",
          "gene_hgnc_id": 10258,
          "hgvs_c": "n.*90A>G",
          "hgvs_p": null,
          "transcript": "ENST00000551975.5",
          "protein_id": "ENSP00000449482.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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      "dbsnp": "rs1555427497",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
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      "gnomad_exomes_homalt": null,
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      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8988803029060364,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.8939999938011169,
      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.937,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9893,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.57,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 8.017,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.991269663302772,
      "dbscsnv_ada_prediction": "Pathogenic",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3,PP5",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
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          "criteria": [
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            "PP3",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_134260.3",
          "gene_symbol": "RORA",
          "hgnc_id": 10258,
          "effects": [
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          "inheritance_mode": "AD",
          "hgvs_c": "c.380A>G",
          "hgvs_p": "p.Lys127Arg"
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        {
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          "criteria": [
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            "PP3",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000559203.1",
          "gene_symbol": "RORA-AS1",
          "hgnc_id": 51410,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.235+11472T>C",
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      ],
      "clinvar_disease": "Intellectual developmental disorder with or without epilepsy or cerebellar ataxia",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Intellectual developmental disorder with or without epilepsy or cerebellar ataxia",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}