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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 15-68146593-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=15&pos=68146593&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "message": null,
  "variants": [
    {
      "acmg_by_gene": [
        {
          "benign_score": 4,
          "criteria": [
            "BS2"
          ],
          "effects": [
            "missense_variant"
          ],
          "gene_symbol": "PIAS1",
          "hgnc_id": 2752,
          "hgvs_c": "c.727G>A",
          "hgvs_p": "p.Val243Met",
          "inheritance_mode": "AD",
          "pathogenic_score": 0,
          "score": -4,
          "transcript": "NM_001320687.1",
          "verdict": "Likely_benign"
        }
      ],
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BS2",
      "acmg_score": -4,
      "allele_count_reference_population": 88,
      "alphamissense_prediction": null,
      "alphamissense_score": 0.1882,
      "alt": "A",
      "apogee2_prediction": null,
      "apogee2_score": null,
      "bayesdelnoaf_prediction": "Benign",
      "bayesdelnoaf_score": -0.26,
      "chr": "15",
      "clinvar_classification": "Uncertain significance",
      "clinvar_disease": "not specified",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "computational_prediction_selected": "Uncertain_significance",
      "computational_score_selected": 0.5536484122276306,
      "computational_source_selected": "MetaRNN",
      "consequences": [
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 651,
          "aa_ref": "V",
          "aa_start": 241,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 7980,
          "cdna_start": 733,
          "cds_end": null,
          "cds_length": 1956,
          "cds_start": 721,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "NM_016166.3",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.721G>A",
          "hgvs_p": "p.Val241Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000249636.11",
          "protein_coding": true,
          "protein_id": "NP_057250.1",
          "strand": true,
          "transcript": "NM_016166.3",
          "transcript_support_level": null
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 651,
          "aa_ref": "V",
          "aa_start": 241,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 7980,
          "cdna_start": 733,
          "cds_end": null,
          "cds_length": 1956,
          "cds_start": 721,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "ENST00000249636.11",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.721G>A",
          "hgvs_p": "p.Val241Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_016166.3",
          "protein_coding": true,
          "protein_id": "ENSP00000249636.6",
          "strand": true,
          "transcript": "ENST00000249636.11",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 697,
          "aa_ref": "V",
          "aa_start": 241,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3285,
          "cdna_start": 747,
          "cds_end": null,
          "cds_length": 2094,
          "cds_start": 721,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 15,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "ENST00000899735.1",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.721G>A",
          "hgvs_p": "p.Val241Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000569794.1",
          "strand": true,
          "transcript": "ENST00000899735.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 685,
          "aa_ref": "V",
          "aa_start": 275,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2299,
          "cdna_start": 835,
          "cds_end": null,
          "cds_length": 2058,
          "cds_start": 823,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 15,
          "exon_rank": 7,
          "exon_rank_end": null,
          "feature": "ENST00000899737.1",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.823G>A",
          "hgvs_p": "p.Val275Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000569796.1",
          "strand": true,
          "transcript": "ENST00000899737.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 681,
          "aa_ref": "V",
          "aa_start": 241,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2534,
          "cdna_start": 733,
          "cds_end": null,
          "cds_length": 2046,
          "cds_start": 721,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 15,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "ENST00000899736.1",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.721G>A",
          "hgvs_p": "p.Val241Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000569795.1",
          "strand": true,
          "transcript": "ENST00000899736.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 653,
          "aa_ref": "V",
          "aa_start": 243,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 6332,
          "cdna_start": 1468,
          "cds_end": null,
          "cds_length": 1962,
          "cds_start": 727,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 15,
          "exon_rank": 7,
          "exon_rank_end": null,
          "feature": "NM_001320687.1",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.727G>A",
          "hgvs_p": "p.Val243Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001307616.1",
          "strand": true,
          "transcript": "NM_001320687.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 653,
          "aa_ref": "V",
          "aa_start": 243,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2932,
          "cdna_start": 1468,
          "cds_end": null,
          "cds_length": 1962,
          "cds_start": 727,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 15,
          "exon_rank": 7,
          "exon_rank_end": null,
          "feature": "ENST00000545237.1",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.727G>A",
          "hgvs_p": "p.Val243Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000438574.1",
          "strand": true,
          "transcript": "ENST00000545237.1",
          "transcript_support_level": 2
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 649,
          "aa_ref": "V",
          "aa_start": 241,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3814,
          "cdna_start": 734,
          "cds_end": null,
          "cds_length": 1950,
          "cds_start": 721,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "ENST00000937336.1",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.721G>A",
          "hgvs_p": "p.Val241Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000607395.1",
          "strand": true,
          "transcript": "ENST00000937336.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 547,
          "aa_ref": "V",
          "aa_start": 241,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1896,
          "cdna_start": 733,
          "cds_end": null,
          "cds_length": 1644,
          "cds_start": 721,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 12,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "ENST00000964577.1",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.721G>A",
          "hgvs_p": "p.Val241Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000634636.1",
          "strand": true,
          "transcript": "ENST00000964577.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 660,
          "aa_ref": "V",
          "aa_start": 250,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 9084,
          "cdna_start": 1837,
          "cds_end": null,
          "cds_length": 1983,
          "cds_start": 748,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 16,
          "exon_rank": 8,
          "exon_rank_end": null,
          "feature": "XM_011522126.3",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.748G>A",
          "hgvs_p": "p.Val250Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_011520428.1",
          "strand": true,
          "transcript": "XM_011522126.3",
          "transcript_support_level": null
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 653,
          "aa_ref": "V",
          "aa_start": 243,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 9543,
          "cdna_start": 2296,
          "cds_end": null,
          "cds_length": 1962,
          "cds_start": 727,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "XM_017022688.2",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.727G>A",
          "hgvs_p": "p.Val243Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_016878177.1",
          "strand": true,
          "transcript": "XM_017022688.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 645,
          "aa_ref": "V",
          "aa_start": 235,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 8600,
          "cdna_start": 1353,
          "cds_end": null,
          "cds_length": 1938,
          "cds_start": 703,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "XM_011522127.3",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.703G>A",
          "hgvs_p": "p.Val235Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_011520429.1",
          "strand": true,
          "transcript": "XM_011522127.3",
          "transcript_support_level": null
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 644,
          "aa_ref": "V",
          "aa_start": 234,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 8868,
          "cdna_start": 1621,
          "cds_end": null,
          "cds_length": 1935,
          "cds_start": 700,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "XM_017022689.2",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.700G>A",
          "hgvs_p": "p.Val234Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_016878178.1",
          "strand": true,
          "transcript": "XM_017022689.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "M",
          "aa_end": null,
          "aa_length": 467,
          "aa_ref": "V",
          "aa_start": 57,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 7545,
          "cdna_start": 298,
          "cds_end": null,
          "cds_length": 1404,
          "cds_start": 169,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 13,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "XM_047433197.1",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.169G>A",
          "hgvs_p": "p.Val57Met",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_047289153.1",
          "strand": true,
          "transcript": "XM_047433197.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 496,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1732,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 1491,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 9,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000964578.1",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "c.470-18138G>A",
          "hgvs_p": null,
          "intron_rank": 2,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000634637.1",
          "strand": true,
          "transcript": "ENST00000964578.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "retained_intron",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 411,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_count": 2,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000564009.1",
          "gene_hgnc_id": 2752,
          "gene_symbol": "PIAS1",
          "hgvs_c": "n.-69G>A",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "ENST00000564009.1",
          "transcript_support_level": 3
        }
      ],
      "custom_annotations": null,
      "dbscsnv_ada_prediction": null,
      "dbscsnv_ada_score": null,
      "dbsnp": "rs763915443",
      "effect": "missense_variant",
      "frequency_reference_population": 0.000054573848,
      "gene_hgnc_id": 2752,
      "gene_symbol": "PIAS1",
      "gnomad_exomes_ac": 85,
      "gnomad_exomes_af": 0.0000582047,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_ac": 3,
      "gnomad_genomes_af": 0.00001972,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_heteroplasmic": null,
      "gnomad_mito_homoplasmic": null,
      "hom_count_reference_population": 0,
      "mitotip_prediction": null,
      "mitotip_score": null,
      "pathogenicity_classification_combined": "Uncertain significance",
      "phenotype_combined": "not specified",
      "phylop100way_prediction": "Pathogenic",
      "phylop100way_score": 10.003,
      "pos": 68146593,
      "ref": "G",
      "revel_prediction": "Benign",
      "revel_score": 0.272,
      "splice_prediction_selected": "Benign",
      "splice_score_selected": 0,
      "splice_source_selected": "max_spliceai",
      "spliceai_max_prediction": "Benign",
      "spliceai_max_score": 0,
      "transcript": "NM_001320687.1"
    }
  ]
}
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