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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 15-89330184-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=15&pos=89330184&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "15",
      "pos": 89330184,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_002693.3",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "c.752C>T",
          "hgvs_p": "p.Thr251Ile",
          "transcript": "NM_002693.3",
          "protein_id": "NP_002684.1",
          "transcript_support_level": null,
          "aa_start": 251,
          "aa_end": null,
          "aa_length": 1239,
          "cds_start": 752,
          "cds_end": null,
          "cds_length": 3720,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000268124.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002693.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "c.752C>T",
          "hgvs_p": "p.Thr251Ile",
          "transcript": "ENST00000268124.11",
          "protein_id": "ENSP00000268124.5",
          "transcript_support_level": 1,
          "aa_start": 251,
          "aa_end": null,
          "aa_length": 1239,
          "cds_start": 752,
          "cds_end": null,
          "cds_length": 3720,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002693.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000268124.11"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "c.752C>T",
          "hgvs_p": "p.Thr251Ile",
          "transcript": "ENST00000442287.6",
          "protein_id": "ENSP00000399851.2",
          "transcript_support_level": 1,
          "aa_start": 251,
          "aa_end": null,
          "aa_length": 1239,
          "cds_start": 752,
          "cds_end": null,
          "cds_length": 3720,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000442287.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "c.752C>T",
          "hgvs_p": "p.Thr251Ile",
          "transcript": "NM_001126131.2",
          "protein_id": "NP_001119603.1",
          "transcript_support_level": null,
          "aa_start": 251,
          "aa_end": null,
          "aa_length": 1239,
          "cds_start": 752,
          "cds_end": null,
          "cds_length": 3720,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001126131.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "c.752C>T",
          "hgvs_p": "p.Thr251Ile",
          "transcript": "ENST00000636937.2",
          "protein_id": "ENSP00000516154.1",
          "transcript_support_level": 5,
          "aa_start": 251,
          "aa_end": null,
          "aa_length": 1239,
          "cds_start": 752,
          "cds_end": null,
          "cds_length": 3720,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000636937.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "n.353C>T",
          "hgvs_p": null,
          "transcript": "ENST00000530292.3",
          "protein_id": "ENSP00000432885.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000530292.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "n.*135C>T",
          "hgvs_p": null,
          "transcript": "ENST00000631044.2",
          "protein_id": "ENSP00000486730.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000631044.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "n.752C>T",
          "hgvs_p": null,
          "transcript": "ENST00000635986.2",
          "protein_id": "ENSP00000490653.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000635986.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "n.752C>T",
          "hgvs_p": null,
          "transcript": "ENST00000636774.1",
          "protein_id": "ENSP00000489799.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000636774.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "n.407C>T",
          "hgvs_p": null,
          "transcript": "ENST00000666746.1",
          "protein_id": "ENSP00000499495.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000666746.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "n.950C>T",
          "hgvs_p": null,
          "transcript": "ENST00000672071.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000672071.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "n.*135C>T",
          "hgvs_p": null,
          "transcript": "ENST00000631044.2",
          "protein_id": "ENSP00000486730.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000631044.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLGARF",
          "gene_hgnc_id": 56246,
          "hgvs_c": "c.*24C>T",
          "hgvs_p": null,
          "transcript": "NM_001430120.1",
          "protein_id": "NP_001417049.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 260,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000706918.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001430120.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLGARF",
          "gene_hgnc_id": 56246,
          "hgvs_c": "c.*24C>T",
          "hgvs_p": null,
          "transcript": "ENST00000706918.1",
          "protein_id": "ENSP00000516626.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 260,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001430120.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000706918.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLG",
          "gene_hgnc_id": 9179,
          "hgvs_c": "c.*27C>T",
          "hgvs_p": null,
          "transcript": "ENST00000637307.1",
          "protein_id": "ENSP00000490427.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 31,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 98,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000637307.1"
        }
      ],
      "gene_symbol": "POLG",
      "gene_hgnc_id": 9179,
      "dbsnp": "rs113994094",
      "frequency_reference_population": 0.0019810582,
      "hom_count_reference_population": 2,
      "allele_count_reference_population": 3197,
      "gnomad_exomes_af": 0.00202882,
      "gnomad_genomes_af": 0.00152287,
      "gnomad_exomes_ac": 2965,
      "gnomad_genomes_ac": 232,
      "gnomad_exomes_homalt": 2,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.13515529036521912,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.527,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.0998,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.01,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 2.591,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PS3,PM1,PP5,BP4",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 1,
          "pathogenic_score": 7,
          "criteria": [
            "PS3",
            "PM1",
            "PP5",
            "BP4"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_002693.3",
          "gene_symbol": "POLG",
          "hgnc_id": 9179,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.752C>T",
          "hgvs_p": "p.Thr251Ile"
        },
        {
          "score": 4,
          "benign_score": 1,
          "pathogenic_score": 5,
          "criteria": [
            "PS3",
            "PP5",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001430120.1",
          "gene_symbol": "POLGARF",
          "hgnc_id": 56246,
          "effects": [
            "downstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.*24C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " and ophthalmoparesis, autosomal dominant 1, autosomal recessive 1, dysarthria,6 conditions,Abnormality of the nervous system,Global developmental delay,Hereditary spastic paraplegia,Hypertrophic cardiomyopathy,Inborn genetic diseases,Mitochondrial DNA depletion syndrome,Mitochondrial DNA depletion syndrome 1,Mitochondrial DNA depletion syndrome 4b,Mitochondrial disease,POLG-Related Spectrum Disorders,POLG-related disorder,Progressive external ophthalmoplegia with mitochondrial DNA deletions,Progressive sclerosing poliodystrophy,See cases,Sensory ataxic neuropathy,Tip-toe gait,not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "P:31 LP:10 US:7 O:3",
      "phenotype_combined": "Mitochondrial DNA depletion syndrome 4b|Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 1|Progressive sclerosing poliodystrophy|not provided|not specified|POLG-Related Spectrum Disorders|Global developmental delay|Mitochondrial DNA depletion syndrome 4b;Progressive sclerosing poliodystrophy|Abnormality of the nervous system|Sensory ataxic neuropathy, dysarthria, and ophthalmoparesis|Mitochondrial disease|Hypertrophic cardiomyopathy|Hereditary spastic paraplegia|Mitochondrial DNA depletion syndrome 1|Mitochondrial DNA depletion syndrome 4b;Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 1;Progressive sclerosing poliodystrophy;Sensory ataxic neuropathy, dysarthria, and ophthalmoparesis|See cases|Mitochondrial DNA depletion syndrome|Mitochondrial DNA depletion syndrome 4b;Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant 1;Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 1;Progressive sclerosing poliodystrophy;Sensory ataxic neuropathy, dysarthria, and ophthalmoparesis|6 conditions|Tip-toe gait|POLG-related disorder|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}
For research and educational, non-commercial use only. Not for clinical or diagnostic use. GeneBe does not provide medical advice. Data use for AI modeling is prohibited: if used, the cost is $0.001 per byte of downloaded uncompressed data.