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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-16159495-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=16159495&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 16159495,
      "ref": "C",
      "alt": "T",
      "effect": "stop_gained",
      "transcript": "NM_001171.6",
      "consequences": [
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3722G>A",
          "hgvs_p": "p.Trp1241*",
          "transcript": "NM_001171.6",
          "protein_id": "NP_001162.5",
          "transcript_support_level": null,
          "aa_start": 1241,
          "aa_end": null,
          "aa_length": 1503,
          "cds_start": 3722,
          "cds_end": null,
          "cds_length": 4512,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000205557.12",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001171.6"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3722G>A",
          "hgvs_p": "p.Trp1241*",
          "transcript": "ENST00000205557.12",
          "protein_id": "ENSP00000205557.7",
          "transcript_support_level": 1,
          "aa_start": 1241,
          "aa_end": null,
          "aa_length": 1503,
          "cds_start": 3722,
          "cds_end": null,
          "cds_length": 4512,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001171.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000205557.12"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3818G>A",
          "hgvs_p": "p.Trp1273*",
          "transcript": "ENST00000909083.1",
          "protein_id": "ENSP00000579142.1",
          "transcript_support_level": null,
          "aa_start": 1273,
          "aa_end": null,
          "aa_length": 1535,
          "cds_start": 3818,
          "cds_end": null,
          "cds_length": 4608,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909083.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3815G>A",
          "hgvs_p": "p.Trp1272*",
          "transcript": "ENST00000909090.1",
          "protein_id": "ENSP00000579149.1",
          "transcript_support_level": null,
          "aa_start": 1272,
          "aa_end": null,
          "aa_length": 1534,
          "cds_start": 3815,
          "cds_end": null,
          "cds_length": 4605,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909090.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3812G>A",
          "hgvs_p": "p.Trp1271*",
          "transcript": "ENST00000909087.1",
          "protein_id": "ENSP00000579146.1",
          "transcript_support_level": null,
          "aa_start": 1271,
          "aa_end": null,
          "aa_length": 1533,
          "cds_start": 3812,
          "cds_end": null,
          "cds_length": 4602,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909087.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3758G>A",
          "hgvs_p": "p.Trp1253*",
          "transcript": "ENST00000909096.1",
          "protein_id": "ENSP00000579155.1",
          "transcript_support_level": null,
          "aa_start": 1253,
          "aa_end": null,
          "aa_length": 1515,
          "cds_start": 3758,
          "cds_end": null,
          "cds_length": 4548,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909096.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3722G>A",
          "hgvs_p": "p.Trp1241*",
          "transcript": "ENST00000909085.1",
          "protein_id": "ENSP00000579144.1",
          "transcript_support_level": null,
          "aa_start": 1241,
          "aa_end": null,
          "aa_length": 1511,
          "cds_start": 3722,
          "cds_end": null,
          "cds_length": 4536,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909085.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3734G>A",
          "hgvs_p": "p.Trp1245*",
          "transcript": "ENST00000909097.1",
          "protein_id": "ENSP00000579156.1",
          "transcript_support_level": null,
          "aa_start": 1245,
          "aa_end": null,
          "aa_length": 1507,
          "cds_start": 3734,
          "cds_end": null,
          "cds_length": 4524,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909097.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3689G>A",
          "hgvs_p": "p.Trp1230*",
          "transcript": "NM_001440309.1",
          "protein_id": "NP_001427238.1",
          "transcript_support_level": null,
          "aa_start": 1230,
          "aa_end": null,
          "aa_length": 1492,
          "cds_start": 3689,
          "cds_end": null,
          "cds_length": 4479,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001440309.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3689G>A",
          "hgvs_p": "p.Trp1230*",
          "transcript": "ENST00000909086.1",
          "protein_id": "ENSP00000579145.1",
          "transcript_support_level": null,
          "aa_start": 1230,
          "aa_end": null,
          "aa_length": 1492,
          "cds_start": 3689,
          "cds_end": null,
          "cds_length": 4479,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909086.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3683G>A",
          "hgvs_p": "p.Trp1228*",
          "transcript": "ENST00000909094.1",
          "protein_id": "ENSP00000579153.1",
          "transcript_support_level": null,
          "aa_start": 1228,
          "aa_end": null,
          "aa_length": 1490,
          "cds_start": 3683,
          "cds_end": null,
          "cds_length": 4473,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909094.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3671G>A",
          "hgvs_p": "p.Trp1224*",
          "transcript": "ENST00000909095.1",
          "protein_id": "ENSP00000579154.1",
          "transcript_support_level": null,
          "aa_start": 1224,
          "aa_end": null,
          "aa_length": 1486,
          "cds_start": 3671,
          "cds_end": null,
          "cds_length": 4461,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909095.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3647G>A",
          "hgvs_p": "p.Trp1216*",
          "transcript": "ENST00000909091.1",
          "protein_id": "ENSP00000579150.1",
          "transcript_support_level": null,
          "aa_start": 1216,
          "aa_end": null,
          "aa_length": 1478,
          "cds_start": 3647,
          "cds_end": null,
          "cds_length": 4437,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909091.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3641G>A",
          "hgvs_p": "p.Trp1214*",
          "transcript": "ENST00000909089.1",
          "protein_id": "ENSP00000579148.1",
          "transcript_support_level": null,
          "aa_start": 1214,
          "aa_end": null,
          "aa_length": 1476,
          "cds_start": 3641,
          "cds_end": null,
          "cds_length": 4431,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909089.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3629G>A",
          "hgvs_p": "p.Trp1210*",
          "transcript": "ENST00000909092.1",
          "protein_id": "ENSP00000579151.1",
          "transcript_support_level": null,
          "aa_start": 1210,
          "aa_end": null,
          "aa_length": 1472,
          "cds_start": 3629,
          "cds_end": null,
          "cds_length": 4419,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909092.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3620G>A",
          "hgvs_p": "p.Trp1207*",
          "transcript": "ENST00000946285.1",
          "protein_id": "ENSP00000616344.1",
          "transcript_support_level": null,
          "aa_start": 1207,
          "aa_end": null,
          "aa_length": 1469,
          "cds_start": 3620,
          "cds_end": null,
          "cds_length": 4410,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946285.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3596G>A",
          "hgvs_p": "p.Trp1199*",
          "transcript": "ENST00000909088.1",
          "protein_id": "ENSP00000579147.1",
          "transcript_support_level": null,
          "aa_start": 1199,
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          "aa_length": 1461,
          "cds_start": 3596,
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          "cds_length": 4386,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000909088.1"
        },
        {
          "aa_ref": "W",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3590G>A",
          "hgvs_p": "p.Trp1197*",
          "transcript": "ENST00000909093.1",
          "protein_id": "ENSP00000579152.1",
          "transcript_support_level": null,
          "aa_start": 1197,
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          "aa_length": 1459,
          "cds_start": 3590,
          "cds_end": null,
          "cds_length": 4380,
          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3578G>A",
          "hgvs_p": "p.Trp1193*",
          "transcript": "ENST00000946284.1",
          "protein_id": "ENSP00000616343.1",
          "transcript_support_level": null,
          "aa_start": 1193,
          "aa_end": null,
          "aa_length": 1455,
          "cds_start": 3578,
          "cds_end": null,
          "cds_length": 4368,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946284.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCC6",
          "gene_hgnc_id": 57,
          "hgvs_c": "c.3554G>A",
          "hgvs_p": "p.Trp1185*",
          "transcript": "NM_001440310.1",
          "protein_id": "NP_001427239.1",
          "transcript_support_level": null,
          "aa_start": 1185,
          "aa_end": null,
          "aa_length": 1447,
          "cds_start": 3554,
          "cds_end": null,
          "cds_length": 4344,
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      "dbsnp": "rs72653748",
      "frequency_reference_population": 0.0000030980202,
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      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.0000027363,
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      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
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      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.28999999165534973,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0.14000000059604645,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "alphamissense_score": null,
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      "bayesdelnoaf_score": 0.29,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": -1.629,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.14,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
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      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 18,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Very_Strong",
      "acmg_by_gene": [
        {
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          "benign_score": 0,
          "pathogenic_score": 18,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5_Very_Strong"
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          "verdict": "Pathogenic",
          "transcript": "NM_001171.6",
          "gene_symbol": "ABCC6",
          "hgnc_id": 57,
          "effects": [
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          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.3722G>A",
          "hgvs_p": "p.Trp1241*"
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      ],
      "clinvar_disease": "Autosomal recessive inherited pseudoxanthoma elasticum,not provided",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:1 LP:1",
      "phenotype_combined": "Autosomal recessive inherited pseudoxanthoma elasticum|not provided",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}