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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-1945855-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=1945855&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 1945855,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_005061.3",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1027C>T",
          "hgvs_p": "p.Arg343Trp",
          "transcript": "NM_005061.3",
          "protein_id": "NP_005052.1",
          "transcript_support_level": null,
          "aa_start": 343,
          "aa_end": null,
          "aa_length": 407,
          "cds_start": 1027,
          "cds_end": null,
          "cds_length": 1224,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000268661.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005061.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1027C>T",
          "hgvs_p": "p.Arg343Trp",
          "transcript": "ENST00000268661.8",
          "protein_id": "ENSP00000268661.7",
          "transcript_support_level": 1,
          "aa_start": 343,
          "aa_end": null,
          "aa_length": 407,
          "cds_start": 1027,
          "cds_end": null,
          "cds_length": 1224,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_005061.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000268661.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1102C>T",
          "hgvs_p": "p.Arg368Trp",
          "transcript": "ENST00000968104.1",
          "protein_id": "ENSP00000638163.1",
          "transcript_support_level": null,
          "aa_start": 368,
          "aa_end": null,
          "aa_length": 432,
          "cds_start": 1102,
          "cds_end": null,
          "cds_length": 1299,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968104.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1087C>T",
          "hgvs_p": "p.Arg363Trp",
          "transcript": "ENST00000968108.1",
          "protein_id": "ENSP00000638167.1",
          "transcript_support_level": null,
          "aa_start": 363,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": 1087,
          "cds_end": null,
          "cds_length": 1284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968108.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1042C>T",
          "hgvs_p": "p.Arg348Trp",
          "transcript": "ENST00000902258.1",
          "protein_id": "ENSP00000572317.1",
          "transcript_support_level": null,
          "aa_start": 348,
          "aa_end": null,
          "aa_length": 412,
          "cds_start": 1042,
          "cds_end": null,
          "cds_length": 1239,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902258.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1033C>T",
          "hgvs_p": "p.Arg345Trp",
          "transcript": "ENST00000968106.1",
          "protein_id": "ENSP00000638165.1",
          "transcript_support_level": null,
          "aa_start": 345,
          "aa_end": null,
          "aa_length": 409,
          "cds_start": 1033,
          "cds_end": null,
          "cds_length": 1230,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968106.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1015C>T",
          "hgvs_p": "p.Arg339Trp",
          "transcript": "ENST00000968111.1",
          "protein_id": "ENSP00000638170.1",
          "transcript_support_level": null,
          "aa_start": 339,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 1015,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968111.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1003C>T",
          "hgvs_p": "p.Arg335Trp",
          "transcript": "ENST00000968109.1",
          "protein_id": "ENSP00000638168.1",
          "transcript_support_level": null,
          "aa_start": 335,
          "aa_end": null,
          "aa_length": 399,
          "cds_start": 1003,
          "cds_end": null,
          "cds_length": 1200,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968109.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1000C>T",
          "hgvs_p": "p.Arg334Trp",
          "transcript": "ENST00000968103.1",
          "protein_id": "ENSP00000638162.1",
          "transcript_support_level": null,
          "aa_start": 334,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 1000,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968103.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1000C>T",
          "hgvs_p": "p.Arg334Trp",
          "transcript": "ENST00000968112.1",
          "protein_id": "ENSP00000638171.1",
          "transcript_support_level": null,
          "aa_start": 334,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 1000,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968112.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.943C>T",
          "hgvs_p": "p.Arg315Trp",
          "transcript": "ENST00000968110.1",
          "protein_id": "ENSP00000638169.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 379,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1140,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968110.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.925C>T",
          "hgvs_p": "p.Arg309Trp",
          "transcript": "ENST00000968105.1",
          "protein_id": "ENSP00000638164.1",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 373,
          "cds_start": 925,
          "cds_end": null,
          "cds_length": 1122,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968105.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1042C>T",
          "hgvs_p": "p.Arg348Trp",
          "transcript": "ENST00000968107.1",
          "protein_id": "ENSP00000638166.1",
          "transcript_support_level": null,
          "aa_start": 348,
          "aa_end": null,
          "aa_length": 372,
          "cds_start": 1042,
          "cds_end": null,
          "cds_length": 1119,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968107.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1027C>T",
          "hgvs_p": "p.Arg343Trp",
          "transcript": "ENST00000902259.1",
          "protein_id": "ENSP00000572318.1",
          "transcript_support_level": null,
          "aa_start": 343,
          "aa_end": null,
          "aa_length": 367,
          "cds_start": 1027,
          "cds_end": null,
          "cds_length": 1104,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902259.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL3L",
          "gene_hgnc_id": 10351,
          "hgvs_c": "c.1042C>T",
          "hgvs_p": "p.Arg348Trp",
          "transcript": "XM_011522571.3",
          "protein_id": "XP_011520873.1",
          "transcript_support_level": null,
          "aa_start": 348,
          "aa_end": null,
          "aa_length": 412,
          "cds_start": 1042,
          "cds_end": null,
          "cds_length": 1239,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011522571.3"
        }
      ],
      "gene_symbol": "RPL3L",
      "gene_hgnc_id": 10351,
      "dbsnp": "rs1465045317",
      "frequency_reference_population": 0.000011772477,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 19,
      "gnomad_exomes_af": 0.0000102623,
      "gnomad_genomes_af": 0.0000262677,
      "gnomad_exomes_ac": 15,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9556159973144531,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.6200000047683716,
      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.531,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.5721,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.09,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 1.574,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.62,
      "spliceai_max_prediction": "Pathogenic",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3,PP5",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_005061.3",
          "gene_symbol": "RPL3L",
          "hgnc_id": 10351,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1027C>T",
          "hgvs_p": "p.Arg343Trp"
        }
      ],
      "clinvar_disease": " 2D, dilated,Cardiomyopathy",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Cardiomyopathy, dilated, 2D",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}