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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-20319726-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=20319726&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 20319726,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001007240.3",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "c.901G>A",
          "hgvs_p": "p.Val301Ile",
          "transcript": "NM_001502.4",
          "protein_id": "NP_001493.2",
          "transcript_support_level": null,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 534,
          "cds_start": 901,
          "cds_end": null,
          "cds_length": 1605,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000302555.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001502.4"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "c.901G>A",
          "hgvs_p": "p.Val301Ile",
          "transcript": "ENST00000302555.10",
          "protein_id": "ENSP00000304044.6",
          "transcript_support_level": 1,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 534,
          "cds_start": 901,
          "cds_end": null,
          "cds_length": 1605,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001502.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000302555.10"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "c.910G>A",
          "hgvs_p": "p.Val304Ile",
          "transcript": "ENST00000381362.8",
          "protein_id": "ENSP00000370767.4",
          "transcript_support_level": 1,
          "aa_start": 304,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 910,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000381362.8"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "c.469G>A",
          "hgvs_p": "p.Val157Ile",
          "transcript": "ENST00000381360.9",
          "protein_id": "ENSP00000370765.5",
          "transcript_support_level": 1,
          "aa_start": 157,
          "aa_end": null,
          "aa_length": 390,
          "cds_start": 469,
          "cds_end": null,
          "cds_length": 1173,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000381360.9"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "c.460G>A",
          "hgvs_p": "p.Val154Ile",
          "transcript": "ENST00000341642.9",
          "protein_id": "ENSP00000343861.5",
          "transcript_support_level": 1,
          "aa_start": 154,
          "aa_end": null,
          "aa_length": 387,
          "cds_start": 460,
          "cds_end": null,
          "cds_length": 1164,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000341642.9"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "c.910G>A",
          "hgvs_p": "p.Val304Ile",
          "transcript": "NM_001007240.3",
          "protein_id": "NP_001007241.2",
          "transcript_support_level": null,
          "aa_start": 304,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 910,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001007240.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "c.901G>A",
          "hgvs_p": "p.Val301Ile",
          "transcript": "ENST00000888890.1",
          "protein_id": "ENSP00000558949.1",
          "transcript_support_level": null,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 534,
          "cds_start": 901,
          "cds_end": null,
          "cds_length": 1605,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000888890.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "c.901G>A",
          "hgvs_p": "p.Val301Ile",
          "transcript": "ENST00000888891.1",
          "protein_id": "ENSP00000558950.1",
          "transcript_support_level": null,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 534,
          "cds_start": 901,
          "cds_end": null,
          "cds_length": 1605,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000888891.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "c.469G>A",
          "hgvs_p": "p.Val157Ile",
          "transcript": "NM_001007241.3",
          "protein_id": "NP_001007242.2",
          "transcript_support_level": null,
          "aa_start": 157,
          "aa_end": null,
          "aa_length": 390,
          "cds_start": 469,
          "cds_end": null,
          "cds_length": 1173,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001007241.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "c.460G>A",
          "hgvs_p": "p.Val154Ile",
          "transcript": "NM_001007242.3",
          "protein_id": "NP_001007243.2",
          "transcript_support_level": null,
          "aa_start": 154,
          "aa_end": null,
          "aa_length": 387,
          "cds_start": 460,
          "cds_end": null,
          "cds_length": 1164,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001007242.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "c.460G>A",
          "hgvs_p": "p.Val154Ile",
          "transcript": "ENST00000888892.1",
          "protein_id": "ENSP00000558951.1",
          "transcript_support_level": null,
          "aa_start": 154,
          "aa_end": null,
          "aa_length": 387,
          "cds_start": 460,
          "cds_end": null,
          "cds_length": 1164,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000888892.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "n.435G>A",
          "hgvs_p": null,
          "transcript": "ENST00000573897.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000573897.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GP2",
          "gene_hgnc_id": 4441,
          "hgvs_c": "c.*25G>A",
          "hgvs_p": null,
          "transcript": "ENST00000575582.5",
          "protein_id": "ENSP00000460466.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 144,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 435,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000575582.5"
        }
      ],
      "gene_symbol": "GP2",
      "gene_hgnc_id": 4441,
      "dbsnp": "rs201741257",
      "frequency_reference_population": 0.000034742687,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 56,
      "gnomad_exomes_af": 0.000018498,
      "gnomad_genomes_af": 0.000190494,
      "gnomad_exomes_ac": 27,
      "gnomad_genomes_ac": 29,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.026813805103302002,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.11999999731779099,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.297,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1167,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.4,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.04,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.12,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001007240.3",
          "gene_symbol": "GP2",
          "hgnc_id": 4441,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.910G>A",
          "hgvs_p": "p.Val304Ile"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}