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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-20737199-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=20737199&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 20737199,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_017736.5",
      "consequences": [
        {
          "aa_ref": "C",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "THUMPD1",
          "gene_hgnc_id": 23807,
          "hgvs_c": "c.743G>T",
          "hgvs_p": "p.Cys248Phe",
          "transcript": "NM_017736.5",
          "protein_id": "NP_060206.2",
          "transcript_support_level": null,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 743,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000396083.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017736.5"
        },
        {
          "aa_ref": "C",
          "aa_alt": "F",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "THUMPD1",
          "gene_hgnc_id": 23807,
          "hgvs_c": "c.743G>T",
          "hgvs_p": "p.Cys248Phe",
          "transcript": "ENST00000396083.7",
          "protein_id": "ENSP00000379392.2",
          "transcript_support_level": 1,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 743,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_017736.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000396083.7"
        },
        {
          "aa_ref": "C",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "THUMPD1",
          "gene_hgnc_id": 23807,
          "hgvs_c": "c.743G>T",
          "hgvs_p": "p.Cys248Phe",
          "transcript": "ENST00000381337.6",
          "protein_id": "ENSP00000370741.2",
          "transcript_support_level": 1,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 743,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000381337.6"
        },
        {
          "aa_ref": "C",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "THUMPD1",
          "gene_hgnc_id": 23807,
          "hgvs_c": "c.743G>T",
          "hgvs_p": "p.Cys248Phe",
          "transcript": "NM_001304550.2",
          "protein_id": "NP_001291479.1",
          "transcript_support_level": null,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 743,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001304550.2"
        },
        {
          "aa_ref": "C",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "THUMPD1",
          "gene_hgnc_id": 23807,
          "hgvs_c": "c.743G>T",
          "hgvs_p": "p.Cys248Phe",
          "transcript": "ENST00000888379.1",
          "protein_id": "ENSP00000558438.1",
          "transcript_support_level": null,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 743,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000888379.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "THUMPD1",
          "gene_hgnc_id": 23807,
          "hgvs_c": "c.650G>T",
          "hgvs_p": "p.Cys217Phe",
          "transcript": "ENST00000966612.1",
          "protein_id": "ENSP00000636671.1",
          "transcript_support_level": null,
          "aa_start": 217,
          "aa_end": null,
          "aa_length": 322,
          "cds_start": 650,
          "cds_end": null,
          "cds_length": 969,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966612.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "THUMPD1",
          "gene_hgnc_id": 23807,
          "hgvs_c": "c.566G>T",
          "hgvs_p": "p.Cys189Phe",
          "transcript": "ENST00000888380.1",
          "protein_id": "ENSP00000558439.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 294,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 885,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000888380.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "ACSM3",
          "gene_hgnc_id": 10522,
          "hgvs_c": "c.-242+7943C>A",
          "hgvs_p": null,
          "transcript": "ENST00000966458.1",
          "protein_id": "ENSP00000636517.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966458.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ACSM3",
          "gene_hgnc_id": 10522,
          "hgvs_c": "c.-293-12066C>A",
          "hgvs_p": null,
          "transcript": "ENST00000887872.1",
          "protein_id": "ENSP00000557931.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1761,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887872.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ACSM3",
          "gene_hgnc_id": 10522,
          "hgvs_c": "c.-189-12711C>A",
          "hgvs_p": null,
          "transcript": "ENST00000887873.1",
          "protein_id": "ENSP00000557932.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": null,
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          "cds_length": 1761,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000887873.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ACSM3",
          "gene_hgnc_id": 10522,
          "hgvs_c": "c.-189-12711C>A",
          "hgvs_p": null,
          "transcript": "ENST00000887874.1",
          "protein_id": "ENSP00000557933.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": null,
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          "cds_length": 1761,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887874.1"
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        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "ACSM3",
          "gene_hgnc_id": 10522,
          "hgvs_c": "c.-189-12711C>A",
          "hgvs_p": null,
          "transcript": "ENST00000966442.1",
          "protein_id": "ENSP00000636501.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 586,
          "cds_start": null,
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          "cdna_start": null,
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          "cdna_length": null,
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        {
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": null,
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          "exon_count": 18,
          "intron_rank": 3,
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          "gene_symbol": "ACSM3",
          "gene_hgnc_id": 10522,
          "hgvs_c": "c.-190+7943C>A",
          "hgvs_p": null,
          "transcript": "ENST00000966443.1",
          "protein_id": "ENSP00000636502.1",
          "transcript_support_level": null,
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          "biotype": "protein_coding",
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        {
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          "canonical": false,
          "protein_coding": true,
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          "consequences": [
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          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "ACSM3",
          "gene_hgnc_id": 10522,
          "hgvs_c": "c.-397+7646C>A",
          "hgvs_p": null,
          "transcript": "ENST00000966445.1",
          "protein_id": "ENSP00000636504.1",
          "transcript_support_level": null,
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          "cds_start": null,
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        {
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          "gene_symbol": "ACSM3",
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          "hgvs_c": "c.-294+7646C>A",
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          "transcript": "ENST00000966446.1",
          "protein_id": "ENSP00000636505.1",
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          "biotype": "protein_coding",
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        {
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          "canonical": false,
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": null,
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          "exon_count": 18,
          "intron_rank": 2,
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          "gene_symbol": "ACSM3",
          "gene_hgnc_id": 10522,
          "hgvs_c": "c.-294+7646C>A",
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          "transcript": "ENST00000966447.1",
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        {
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          "consequences": [
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          "exon_count": 19,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ACSM3",
          "gene_hgnc_id": 10522,
          "hgvs_c": "c.-396-12066C>A",
          "hgvs_p": null,
          "transcript": "ENST00000966448.1",
          "protein_id": "ENSP00000636507.1",
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          "gene_symbol": "ACSM3",
          "gene_hgnc_id": 10522,
          "hgvs_c": "c.-95-18374C>A",
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        {
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          "intron_rank": 1,
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          "gene_symbol": "ACSM3",
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          "hgvs_c": "c.-293-12066C>A",
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          "biotype": "protein_coding",
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        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "ACSM3",
          "gene_hgnc_id": 10522,
          "hgvs_c": "c.-189-12711C>A",
          "hgvs_p": null,
          "transcript": "ENST00000966451.1",
          "protein_id": "ENSP00000636510.1",
          "transcript_support_level": null,
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          "aa_length": 586,
          "cds_start": null,
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          "mane_select": null,
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        },
        {
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      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_017736.5",
          "gene_symbol": "THUMPD1",
          "hgnc_id": 23807,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.743G>T",
          "hgvs_p": "p.Cys248Phe"
        },
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000568235.5",
          "gene_symbol": "ACSM3",
          "hgnc_id": 10522,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-189-12711C>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Inborn genetic diseases",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}