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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-2090335-ACTC-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=2090335&ref=ACTC&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 2090335,
      "ref": "ACTC",
      "alt": "A",
      "effect": "conservative_inframe_deletion",
      "transcript": "NM_001009944.3",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 45,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.12391_12393delGAG",
          "hgvs_p": "p.Glu4131del",
          "transcript": "NM_001009944.3",
          "protein_id": "NP_001009944.3",
          "transcript_support_level": null,
          "aa_start": 4131,
          "aa_end": null,
          "aa_length": 4303,
          "cds_start": 12391,
          "cds_end": null,
          "cds_length": 12912,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000262304.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001009944.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 45,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.12391_12393delGAG",
          "hgvs_p": "p.Glu4131del",
          "transcript": "ENST00000262304.9",
          "protein_id": "ENSP00000262304.4",
          "transcript_support_level": 1,
          "aa_start": 4131,
          "aa_end": null,
          "aa_length": 4303,
          "cds_start": 12391,
          "cds_end": null,
          "cds_length": 12912,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001009944.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000262304.9"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 45,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.12388_12390delGAG",
          "hgvs_p": "p.Glu4130del",
          "transcript": "ENST00000423118.5",
          "protein_id": "ENSP00000399501.1",
          "transcript_support_level": 1,
          "aa_start": 4130,
          "aa_end": null,
          "aa_length": 4302,
          "cds_start": 12388,
          "cds_end": null,
          "cds_length": 12909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000423118.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 45,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.12388_12390delGAG",
          "hgvs_p": "p.Glu4130del",
          "transcript": "NM_000296.4",
          "protein_id": "NP_000287.4",
          "transcript_support_level": null,
          "aa_start": 4130,
          "aa_end": null,
          "aa_length": 4302,
          "cds_start": 12388,
          "cds_end": null,
          "cds_length": 12909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000296.4"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 47,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.12526_12528delGAG",
          "hgvs_p": "p.Glu4176del",
          "transcript": "XM_047434208.1",
          "protein_id": "XP_047290164.1",
          "transcript_support_level": null,
          "aa_start": 4176,
          "aa_end": null,
          "aa_length": 4348,
          "cds_start": 12526,
          "cds_end": null,
          "cds_length": 13047,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434208.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 46,
          "exon_rank_end": null,
          "exon_count": 47,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.12454_12456delGAG",
          "hgvs_p": "p.Glu4152del",
          "transcript": "XM_047434209.1",
          "protein_id": "XP_047290165.1",
          "transcript_support_level": null,
          "aa_start": 4152,
          "aa_end": null,
          "aa_length": 4324,
          "cds_start": 12454,
          "cds_end": null,
          "cds_length": 12975,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434209.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 46,
          "exon_rank_end": null,
          "exon_count": 47,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.12445_12447delGAG",
          "hgvs_p": "p.Glu4149del",
          "transcript": "XM_011522528.4",
          "protein_id": "XP_011520830.1",
          "transcript_support_level": null,
          "aa_start": 4149,
          "aa_end": null,
          "aa_length": 4321,
          "cds_start": 12445,
          "cds_end": null,
          "cds_length": 12966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011522528.4"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 46,
          "exon_rank_end": null,
          "exon_count": 47,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.12442_12444delGAG",
          "hgvs_p": "p.Glu4148del",
          "transcript": "XM_011522529.3",
          "protein_id": "XP_011520831.1",
          "transcript_support_level": null,
          "aa_start": 4148,
          "aa_end": null,
          "aa_length": 4320,
          "cds_start": 12442,
          "cds_end": null,
          "cds_length": 12963,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011522529.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 47,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.12373_12375delGAG",
          "hgvs_p": "p.Glu4125del",
          "transcript": "XM_047434210.1",
          "protein_id": "XP_047290166.1",
          "transcript_support_level": null,
          "aa_start": 4125,
          "aa_end": null,
          "aa_length": 4297,
          "cds_start": 12373,
          "cds_end": null,
          "cds_length": 12894,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434210.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 47,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.12316_12318delGAG",
          "hgvs_p": "p.Glu4106del",
          "transcript": "XM_047434211.1",
          "protein_id": "XP_047290167.1",
          "transcript_support_level": null,
          "aa_start": 4106,
          "aa_end": null,
          "aa_length": 4278,
          "cds_start": 12316,
          "cds_end": null,
          "cds_length": 12837,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434211.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 38,
          "exon_rank_end": null,
          "exon_count": 39,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.10486_10488delGAG",
          "hgvs_p": "p.Glu3496del",
          "transcript": "XM_047434212.1",
          "protein_id": "XP_047290168.1",
          "transcript_support_level": null,
          "aa_start": 3496,
          "aa_end": null,
          "aa_length": 3668,
          "cds_start": 10486,
          "cds_end": null,
          "cds_length": 11007,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434212.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 35,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9469_9471delGAG",
          "hgvs_p": "p.Glu3157del",
          "transcript": "XM_011522537.2",
          "protein_id": "XP_011520839.1",
          "transcript_support_level": null,
          "aa_start": 3157,
          "aa_end": null,
          "aa_length": 3329,
          "cds_start": 9469,
          "cds_end": null,
          "cds_length": 9990,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011522537.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 35,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9463_9465delGAG",
          "hgvs_p": "p.Glu3155del",
          "transcript": "XM_047434213.1",
          "protein_id": "XP_047290169.1",
          "transcript_support_level": null,
          "aa_start": 3155,
          "aa_end": null,
          "aa_length": 3327,
          "cds_start": 9463,
          "cds_end": null,
          "cds_length": 9984,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434213.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 34,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9346_9348delGAG",
          "hgvs_p": "p.Glu3116del",
          "transcript": "XM_005255370.4",
          "protein_id": "XP_005255427.1",
          "transcript_support_level": null,
          "aa_start": 3116,
          "aa_end": null,
          "aa_length": 3288,
          "cds_start": 9346,
          "cds_end": null,
          "cds_length": 9867,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005255370.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "n.419_421delGAG",
          "hgvs_p": null,
          "transcript": "ENST00000472577.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000472577.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MIR1225",
          "gene_hgnc_id": 33931,
          "hgvs_c": "n.-54_-52delGAG",
          "hgvs_p": null,
          "transcript": "ENST00000408729.1",
          "protein_id": null,
          "transcript_support_level": 6,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "miRNA",
          "feature": "ENST00000408729.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MIR1225",
          "gene_hgnc_id": 33931,
          "hgvs_c": "n.-54_-52delGAG",
          "hgvs_p": null,
          "transcript": "NR_030646.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_030646.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": null,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MIR1225",
          "gene_hgnc_id": 33931,
          "hgvs_c": "n.-122_-120delGAG",
          "hgvs_p": null,
          "transcript": "unassigned_transcript_2769",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "miRNA",
          "feature": "unassigned_transcript_2769"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": null,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MIR1225",
          "gene_hgnc_id": 33931,
          "hgvs_c": "n.-54_-52delGAG",
          "hgvs_p": null,
          "transcript": "unassigned_transcript_2770",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "miRNA",
          "feature": "unassigned_transcript_2770"
        }
      ],
      "gene_symbol": "PKD1",
      "gene_hgnc_id": 9008,
      "dbsnp": "rs1555444468",
      "frequency_reference_population": 6.8490897e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84909e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": null,
      "computational_prediction_selected": null,
      "computational_source_selected": null,
      "splice_score_selected": 0.3199999928474426,
      "splice_prediction_selected": "Uncertain_significance",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": 4.554,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.32,
      "spliceai_max_prediction": "Uncertain_significance",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM1,PM2,PM4_Supporting,PP5",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM1",
            "PM2",
            "PM4_Supporting",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_001009944.3",
          "gene_symbol": "PKD1",
          "hgnc_id": 9008,
          "effects": [
            "conservative_inframe_deletion"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.12391_12393delGAG",
          "hgvs_p": "p.Glu4131del"
        },
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NR_030646.1",
          "gene_symbol": "MIR1225",
          "hgnc_id": 33931,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.-54_-52delGAG",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Autosomal dominant polycystic kidney disease,Polycystic kidney disease,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "LP:2 US:1",
      "phenotype_combined": "Polycystic kidney disease|not provided|Autosomal dominant polycystic kidney disease",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}