← Back to variant description

GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-20935410-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=20935410&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 20935410,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_017539.2",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 61,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11797G>A",
          "hgvs_p": "p.Val3933Ile",
          "transcript": "NM_001347886.2",
          "protein_id": "NP_001334815.1",
          "transcript_support_level": null,
          "aa_start": 3933,
          "aa_end": null,
          "aa_length": 4070,
          "cds_start": 11797,
          "cds_end": null,
          "cds_length": 12213,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000698260.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001347886.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 61,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11797G>A",
          "hgvs_p": "p.Val3933Ile",
          "transcript": "ENST00000698260.1",
          "protein_id": "ENSP00000513632.1",
          "transcript_support_level": null,
          "aa_start": 3933,
          "aa_end": null,
          "aa_length": 4070,
          "cds_start": 11797,
          "cds_end": null,
          "cds_length": 12213,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001347886.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000698260.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 61,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11935G>A",
          "hgvs_p": "p.Val3979Ile",
          "transcript": "ENST00000261383.3",
          "protein_id": "ENSP00000261383.3",
          "transcript_support_level": 1,
          "aa_start": 3979,
          "aa_end": null,
          "aa_length": 4116,
          "cds_start": 11935,
          "cds_end": null,
          "cds_length": 12351,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000261383.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 61,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11977G>A",
          "hgvs_p": "p.Val3993Ile",
          "transcript": "ENST00000685858.1",
          "protein_id": "ENSP00000508756.1",
          "transcript_support_level": null,
          "aa_start": 3993,
          "aa_end": null,
          "aa_length": 4130,
          "cds_start": 11977,
          "cds_end": null,
          "cds_length": 12393,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000685858.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 61,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11935G>A",
          "hgvs_p": "p.Val3979Ile",
          "transcript": "NM_017539.2",
          "protein_id": "NP_060009.1",
          "transcript_support_level": null,
          "aa_start": 3979,
          "aa_end": null,
          "aa_length": 4116,
          "cds_start": 11935,
          "cds_end": null,
          "cds_length": 12351,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017539.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 61,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11977G>A",
          "hgvs_p": "p.Val3993Ile",
          "transcript": "XM_017023432.2",
          "protein_id": "XP_016878921.1",
          "transcript_support_level": null,
          "aa_start": 3993,
          "aa_end": null,
          "aa_length": 4130,
          "cds_start": 11977,
          "cds_end": null,
          "cds_length": 12393,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023432.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 62,
          "exon_rank_end": null,
          "exon_count": 63,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11818G>A",
          "hgvs_p": "p.Val3940Ile",
          "transcript": "XM_011545882.3",
          "protein_id": "XP_011544184.1",
          "transcript_support_level": null,
          "aa_start": 3940,
          "aa_end": null,
          "aa_length": 4077,
          "cds_start": 11818,
          "cds_end": null,
          "cds_length": 12234,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011545882.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 62,
          "exon_rank_end": null,
          "exon_count": 63,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11818G>A",
          "hgvs_p": "p.Val3940Ile",
          "transcript": "XM_011545883.1",
          "protein_id": "XP_011544185.1",
          "transcript_support_level": null,
          "aa_start": 3940,
          "aa_end": null,
          "aa_length": 4077,
          "cds_start": 11818,
          "cds_end": null,
          "cds_length": 12234,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011545883.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 61,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11797G>A",
          "hgvs_p": "p.Val3933Ile",
          "transcript": "XM_047434348.1",
          "protein_id": "XP_047290304.1",
          "transcript_support_level": null,
          "aa_start": 3933,
          "aa_end": null,
          "aa_length": 4070,
          "cds_start": 11797,
          "cds_end": null,
          "cds_length": 12213,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434348.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 61,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11683G>A",
          "hgvs_p": "p.Val3895Ile",
          "transcript": "XM_017023426.2",
          "protein_id": "XP_016878915.1",
          "transcript_support_level": null,
          "aa_start": 3895,
          "aa_end": null,
          "aa_length": 4032,
          "cds_start": 11683,
          "cds_end": null,
          "cds_length": 12099,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023426.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 60,
          "exon_rank_end": null,
          "exon_count": 61,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11575G>A",
          "hgvs_p": "p.Val3859Ile",
          "transcript": "XM_017023427.2",
          "protein_id": "XP_016878916.1",
          "transcript_support_level": null,
          "aa_start": 3859,
          "aa_end": null,
          "aa_length": 3996,
          "cds_start": 11575,
          "cds_end": null,
          "cds_length": 11991,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023427.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 59,
          "exon_rank_end": null,
          "exon_count": 60,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11554G>A",
          "hgvs_p": "p.Val3852Ile",
          "transcript": "XM_047434349.1",
          "protein_id": "XP_047290305.1",
          "transcript_support_level": null,
          "aa_start": 3852,
          "aa_end": null,
          "aa_length": 3989,
          "cds_start": 11554,
          "cds_end": null,
          "cds_length": 11970,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434349.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 59,
          "exon_rank_end": null,
          "exon_count": 60,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.11239G>A",
          "hgvs_p": "p.Val3747Ile",
          "transcript": "XM_017023428.2",
          "protein_id": "XP_016878917.1",
          "transcript_support_level": null,
          "aa_start": 3747,
          "aa_end": null,
          "aa_length": 3884,
          "cds_start": 11239,
          "cds_end": null,
          "cds_length": 11655,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023428.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 54,
          "exon_rank_end": null,
          "exon_count": 55,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.10807G>A",
          "hgvs_p": "p.Val3603Ile",
          "transcript": "XM_011545886.3",
          "protein_id": "XP_011544188.1",
          "transcript_support_level": null,
          "aa_start": 3603,
          "aa_end": null,
          "aa_length": 3740,
          "cds_start": 10807,
          "cds_end": null,
          "cds_length": 11223,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011545886.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 44,
          "exon_rank_end": null,
          "exon_count": 45,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.9220G>A",
          "hgvs_p": "p.Val3074Ile",
          "transcript": "XM_011545888.2",
          "protein_id": "XP_011544190.1",
          "transcript_support_level": null,
          "aa_start": 3074,
          "aa_end": null,
          "aa_length": 3211,
          "cds_start": 9220,
          "cds_end": null,
          "cds_length": 9636,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011545888.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 44,
          "exon_rank_end": null,
          "exon_count": 45,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.9220G>A",
          "hgvs_p": "p.Val3074Ile",
          "transcript": "XM_011545889.2",
          "protein_id": "XP_011544191.1",
          "transcript_support_level": null,
          "aa_start": 3074,
          "aa_end": null,
          "aa_length": 3211,
          "cds_start": 9220,
          "cds_end": null,
          "cds_length": 9636,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011545889.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.5872G>A",
          "hgvs_p": "p.Val1958Ile",
          "transcript": "XM_017023430.2",
          "protein_id": "XP_016878919.1",
          "transcript_support_level": null,
          "aa_start": 1958,
          "aa_end": null,
          "aa_length": 2095,
          "cds_start": 5872,
          "cds_end": null,
          "cds_length": 6288,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023430.2"
        }
      ],
      "gene_symbol": "DNAH3",
      "gene_hgnc_id": 2949,
      "dbsnp": "rs377222116",
      "frequency_reference_population": 0.00003659033,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 59,
      "gnomad_exomes_af": 0.0000390264,
      "gnomad_genomes_af": 0.0000131667,
      "gnomad_exomes_ac": 57,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.10068288445472717,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.027,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0857,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.7,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.83,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_017539.2",
          "gene_symbol": "DNAH3",
          "hgnc_id": 2949,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.11935G>A",
          "hgvs_p": "p.Val3979Ile"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}