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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-2100470-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=2100470&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 2100470,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001009944.3",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9494T>C",
          "hgvs_p": "p.Leu3165Pro",
          "transcript": "NM_001009944.3",
          "protein_id": "NP_001009944.3",
          "transcript_support_level": null,
          "aa_start": 3165,
          "aa_end": null,
          "aa_length": 4303,
          "cds_start": 9494,
          "cds_end": null,
          "cds_length": 12912,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000262304.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001009944.3"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9494T>C",
          "hgvs_p": "p.Leu3165Pro",
          "transcript": "ENST00000262304.9",
          "protein_id": "ENSP00000262304.4",
          "transcript_support_level": 1,
          "aa_start": 3165,
          "aa_end": null,
          "aa_length": 4303,
          "cds_start": 9494,
          "cds_end": null,
          "cds_length": 12912,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001009944.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000262304.9"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9494T>C",
          "hgvs_p": "p.Leu3165Pro",
          "transcript": "ENST00000423118.5",
          "protein_id": "ENSP00000399501.1",
          "transcript_support_level": 1,
          "aa_start": 3165,
          "aa_end": null,
          "aa_length": 4302,
          "cds_start": 9494,
          "cds_end": null,
          "cds_length": 12909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000423118.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "n.1231T>C",
          "hgvs_p": null,
          "transcript": "ENST00000480227.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000480227.5"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9494T>C",
          "hgvs_p": "p.Leu3165Pro",
          "transcript": "NM_000296.4",
          "protein_id": "NP_000287.4",
          "transcript_support_level": null,
          "aa_start": 3165,
          "aa_end": null,
          "aa_length": 4302,
          "cds_start": 9494,
          "cds_end": null,
          "cds_length": 12909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000296.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.1094T>C",
          "hgvs_p": "p.Leu365Pro",
          "transcript": "ENST00000567946.1",
          "protein_id": "ENSP00000457984.1",
          "transcript_support_level": 5,
          "aa_start": 365,
          "aa_end": null,
          "aa_length": 605,
          "cds_start": 1094,
          "cds_end": null,
          "cds_length": 1820,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000567946.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9629T>C",
          "hgvs_p": "p.Leu3210Pro",
          "transcript": "XM_047434208.1",
          "protein_id": "XP_047290164.1",
          "transcript_support_level": null,
          "aa_start": 3210,
          "aa_end": null,
          "aa_length": 4348,
          "cds_start": 9629,
          "cds_end": null,
          "cds_length": 13047,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434208.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 47,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9557T>C",
          "hgvs_p": "p.Leu3186Pro",
          "transcript": "XM_047434209.1",
          "protein_id": "XP_047290165.1",
          "transcript_support_level": null,
          "aa_start": 3186,
          "aa_end": null,
          "aa_length": 4324,
          "cds_start": 9557,
          "cds_end": null,
          "cds_length": 12975,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434209.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 47,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9548T>C",
          "hgvs_p": "p.Leu3183Pro",
          "transcript": "XM_011522528.4",
          "protein_id": "XP_011520830.1",
          "transcript_support_level": null,
          "aa_start": 3183,
          "aa_end": null,
          "aa_length": 4321,
          "cds_start": 9548,
          "cds_end": null,
          "cds_length": 12966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011522528.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 47,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9548T>C",
          "hgvs_p": "p.Leu3183Pro",
          "transcript": "XM_011522529.3",
          "protein_id": "XP_011520831.1",
          "transcript_support_level": null,
          "aa_start": 3183,
          "aa_end": null,
          "aa_length": 4320,
          "cds_start": 9548,
          "cds_end": null,
          "cds_length": 12963,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011522529.3"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9476T>C",
          "hgvs_p": "p.Leu3159Pro",
          "transcript": "XM_047434210.1",
          "protein_id": "XP_047290166.1",
          "transcript_support_level": null,
          "aa_start": 3159,
          "aa_end": null,
          "aa_length": 4297,
          "cds_start": 9476,
          "cds_end": null,
          "cds_length": 12894,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434210.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.9419T>C",
          "hgvs_p": "p.Leu3140Pro",
          "transcript": "XM_047434211.1",
          "protein_id": "XP_047290167.1",
          "transcript_support_level": null,
          "aa_start": 3140,
          "aa_end": null,
          "aa_length": 4278,
          "cds_start": 9419,
          "cds_end": null,
          "cds_length": 12837,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434211.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 39,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.7589T>C",
          "hgvs_p": "p.Leu2530Pro",
          "transcript": "XM_047434212.1",
          "protein_id": "XP_047290168.1",
          "transcript_support_level": null,
          "aa_start": 2530,
          "aa_end": null,
          "aa_length": 3668,
          "cds_start": 7589,
          "cds_end": null,
          "cds_length": 11007,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434212.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.6572T>C",
          "hgvs_p": "p.Leu2191Pro",
          "transcript": "XM_011522537.2",
          "protein_id": "XP_011520839.1",
          "transcript_support_level": null,
          "aa_start": 2191,
          "aa_end": null,
          "aa_length": 3329,
          "cds_start": 6572,
          "cds_end": null,
          "cds_length": 9990,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011522537.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.6566T>C",
          "hgvs_p": "p.Leu2189Pro",
          "transcript": "XM_047434213.1",
          "protein_id": "XP_047290169.1",
          "transcript_support_level": null,
          "aa_start": 2189,
          "aa_end": null,
          "aa_length": 3327,
          "cds_start": 6566,
          "cds_end": null,
          "cds_length": 9984,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434213.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "c.6449T>C",
          "hgvs_p": "p.Leu2150Pro",
          "transcript": "XM_005255370.4",
          "protein_id": "XP_005255427.1",
          "transcript_support_level": null,
          "aa_start": 2150,
          "aa_end": null,
          "aa_length": 3288,
          "cds_start": 6449,
          "cds_end": null,
          "cds_length": 9867,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005255370.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "n.2573T>C",
          "hgvs_p": null,
          "transcript": "ENST00000415938.7",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000415938.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "n.217T>C",
          "hgvs_p": null,
          "transcript": "ENST00000469851.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000469851.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "n.1138T>C",
          "hgvs_p": null,
          "transcript": "ENST00000471603.6",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000471603.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PKD1",
          "gene_hgnc_id": 9008,
          "hgvs_c": "n.3380T>C",
          "hgvs_p": null,
          "transcript": "ENST00000483731.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
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        {
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          "transcript": "ENST00000570193.5",
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        {
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          "exon_count": 3,
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          "gene_symbol": "PKD1",
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          "hgvs_c": "n.-170T>C",
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          "transcript": "ENST00000570253.5",
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          "biotype": "retained_intron",
          "feature": "ENST00000570253.5"
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      ],
      "gene_symbol": "PKD1",
      "gene_hgnc_id": 9008,
      "dbsnp": "rs1555449597",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9651325941085815,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.776,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.8638,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.27,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 5.824,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 8,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM1,PM2,PP3_Strong",
      "acmg_by_gene": [
        {
          "score": 8,
          "benign_score": 0,
          "pathogenic_score": 8,
          "criteria": [
            "PM1",
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_001009944.3",
          "gene_symbol": "PKD1",
          "hgnc_id": 9008,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.9494T>C",
          "hgvs_p": "p.Leu3165Pro"
        }
      ],
      "clinvar_disease": " adult type,Polycystic kidney disease",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Polycystic kidney disease, adult type",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}