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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-21111703-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=21111703&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 21111703,
      "ref": "A",
      "alt": "G",
      "effect": "synonymous_variant",
      "transcript": "NM_017539.2",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1842T>C",
          "hgvs_p": "p.Asp614Asp",
          "transcript": "NM_001347886.2",
          "protein_id": "NP_001334815.1",
          "transcript_support_level": null,
          "aa_start": 614,
          "aa_end": null,
          "aa_length": 4070,
          "cds_start": 1842,
          "cds_end": null,
          "cds_length": 12213,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000698260.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001347886.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1842T>C",
          "hgvs_p": "p.Asp614Asp",
          "transcript": "ENST00000698260.1",
          "protein_id": "ENSP00000513632.1",
          "transcript_support_level": null,
          "aa_start": 614,
          "aa_end": null,
          "aa_length": 4070,
          "cds_start": 1842,
          "cds_end": null,
          "cds_length": 12213,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001347886.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000698260.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.2022T>C",
          "hgvs_p": "p.Asp674Asp",
          "transcript": "ENST00000261383.3",
          "protein_id": "ENSP00000261383.3",
          "transcript_support_level": 1,
          "aa_start": 674,
          "aa_end": null,
          "aa_length": 4116,
          "cds_start": 2022,
          "cds_end": null,
          "cds_length": 12351,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000261383.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.2022T>C",
          "hgvs_p": "p.Asp674Asp",
          "transcript": "ENST00000685858.1",
          "protein_id": "ENSP00000508756.1",
          "transcript_support_level": null,
          "aa_start": 674,
          "aa_end": null,
          "aa_length": 4130,
          "cds_start": 2022,
          "cds_end": null,
          "cds_length": 12393,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000685858.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.2022T>C",
          "hgvs_p": "p.Asp674Asp",
          "transcript": "NM_017539.2",
          "protein_id": "NP_060009.1",
          "transcript_support_level": null,
          "aa_start": 674,
          "aa_end": null,
          "aa_length": 4116,
          "cds_start": 2022,
          "cds_end": null,
          "cds_length": 12351,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017539.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1842T>C",
          "hgvs_p": "p.Asp614Asp",
          "transcript": "NM_001394581.1",
          "protein_id": "NP_001381510.1",
          "transcript_support_level": null,
          "aa_start": 614,
          "aa_end": null,
          "aa_length": 753,
          "cds_start": 1842,
          "cds_end": null,
          "cds_length": 2262,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001394581.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.2022T>C",
          "hgvs_p": "p.Asp674Asp",
          "transcript": "XM_017023432.2",
          "protein_id": "XP_016878921.1",
          "transcript_support_level": null,
          "aa_start": 674,
          "aa_end": null,
          "aa_length": 4130,
          "cds_start": 2022,
          "cds_end": null,
          "cds_length": 12393,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023432.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 63,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1863T>C",
          "hgvs_p": "p.Asp621Asp",
          "transcript": "XM_011545882.3",
          "protein_id": "XP_011544184.1",
          "transcript_support_level": null,
          "aa_start": 621,
          "aa_end": null,
          "aa_length": 4077,
          "cds_start": 1863,
          "cds_end": null,
          "cds_length": 12234,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011545882.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 63,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1863T>C",
          "hgvs_p": "p.Asp621Asp",
          "transcript": "XM_011545883.1",
          "protein_id": "XP_011544185.1",
          "transcript_support_level": null,
          "aa_start": 621,
          "aa_end": null,
          "aa_length": 4077,
          "cds_start": 1863,
          "cds_end": null,
          "cds_length": 12234,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011545883.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1842T>C",
          "hgvs_p": "p.Asp614Asp",
          "transcript": "XM_047434348.1",
          "protein_id": "XP_047290304.1",
          "transcript_support_level": null,
          "aa_start": 614,
          "aa_end": null,
          "aa_length": 4070,
          "cds_start": 1842,
          "cds_end": null,
          "cds_length": 12213,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434348.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1863T>C",
          "hgvs_p": "p.Asp621Asp",
          "transcript": "XM_017023426.2",
          "protein_id": "XP_016878915.1",
          "transcript_support_level": null,
          "aa_start": 621,
          "aa_end": null,
          "aa_length": 4032,
          "cds_start": 1863,
          "cds_end": null,
          "cds_length": 12099,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023426.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 61,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1620T>C",
          "hgvs_p": "p.Asp540Asp",
          "transcript": "XM_017023427.2",
          "protein_id": "XP_016878916.1",
          "transcript_support_level": null,
          "aa_start": 540,
          "aa_end": null,
          "aa_length": 3996,
          "cds_start": 1620,
          "cds_end": null,
          "cds_length": 11991,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023427.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 60,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1599T>C",
          "hgvs_p": "p.Asp533Asp",
          "transcript": "XM_047434349.1",
          "protein_id": "XP_047290305.1",
          "transcript_support_level": null,
          "aa_start": 533,
          "aa_end": null,
          "aa_length": 3989,
          "cds_start": 1599,
          "cds_end": null,
          "cds_length": 11970,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434349.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 60,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1863T>C",
          "hgvs_p": "p.Asp621Asp",
          "transcript": "XM_017023428.2",
          "protein_id": "XP_016878917.1",
          "transcript_support_level": null,
          "aa_start": 621,
          "aa_end": null,
          "aa_length": 3884,
          "cds_start": 1863,
          "cds_end": null,
          "cds_length": 11655,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023428.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 60,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1863T>C",
          "hgvs_p": "p.Asp621Asp",
          "transcript": "XM_011545885.4",
          "protein_id": "XP_011544187.1",
          "transcript_support_level": null,
          "aa_start": 621,
          "aa_end": null,
          "aa_length": 3771,
          "cds_start": 1863,
          "cds_end": null,
          "cds_length": 11316,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011545885.4"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 55,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.852T>C",
          "hgvs_p": "p.Asp284Asp",
          "transcript": "XM_011545886.3",
          "protein_id": "XP_011544188.1",
          "transcript_support_level": null,
          "aa_start": 284,
          "aa_end": null,
          "aa_length": 3740,
          "cds_start": 852,
          "cds_end": null,
          "cds_length": 11223,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011545886.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 49,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1863T>C",
          "hgvs_p": "p.Asp621Asp",
          "transcript": "XM_017023429.2",
          "protein_id": "XP_016878918.1",
          "transcript_support_level": null,
          "aa_start": 621,
          "aa_end": null,
          "aa_length": 2324,
          "cds_start": 1863,
          "cds_end": null,
          "cds_length": 6975,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023429.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 41,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "c.1863T>C",
          "hgvs_p": "p.Asp621Asp",
          "transcript": "XM_017023431.2",
          "protein_id": "XP_016878920.1",
          "transcript_support_level": null,
          "aa_start": 621,
          "aa_end": null,
          "aa_length": 1848,
          "cds_start": 1863,
          "cds_end": null,
          "cds_length": 5547,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023431.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAH3",
          "gene_hgnc_id": 2949,
          "hgvs_c": "n.2118T>C",
          "hgvs_p": null,
          "transcript": "ENST00000396036.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000396036.5"
        }
      ],
      "gene_symbol": "DNAH3",
      "gene_hgnc_id": 2949,
      "dbsnp": "rs144106056",
      "frequency_reference_population": 0.0006611109,
      "hom_count_reference_population": 7,
      "allele_count_reference_population": 1067,
      "gnomad_exomes_af": 0.000663571,
      "gnomad_genomes_af": 0.000637478,
      "gnomad_exomes_ac": 970,
      "gnomad_genomes_ac": 97,
      "gnomad_exomes_homalt": 7,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.6200000047683716,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.62,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -1.151,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -17,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BP7,BS2",
      "acmg_by_gene": [
        {
          "score": -17,
          "benign_score": 17,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BP7",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_017539.2",
          "gene_symbol": "DNAH3",
          "hgnc_id": 2949,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2022T>C",
          "hgvs_p": "p.Asp674Asp"
        }
      ],
      "clinvar_disease": "not provided,not specified",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:2",
      "phenotype_combined": "not specified|not provided",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}