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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-2152860-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=2152860&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 2152860,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_014353.5",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "c.509T>C",
          "hgvs_p": "p.Val170Ala",
          "transcript": "NM_014353.5",
          "protein_id": "NP_055168.2",
          "transcript_support_level": null,
          "aa_start": 170,
          "aa_end": null,
          "aa_length": 256,
          "cds_start": 509,
          "cds_end": null,
          "cds_length": 771,
          "cdna_start": 668,
          "cdna_end": null,
          "cdna_length": 1674,
          "mane_select": "ENST00000210187.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_014353.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "c.509T>C",
          "hgvs_p": "p.Val170Ala",
          "transcript": "ENST00000210187.11",
          "protein_id": "ENSP00000210187.6",
          "transcript_support_level": 1,
          "aa_start": 170,
          "aa_end": null,
          "aa_length": 256,
          "cds_start": 509,
          "cds_end": null,
          "cds_length": 771,
          "cdna_start": 668,
          "cdna_end": null,
          "cdna_length": 1674,
          "mane_select": "NM_014353.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000210187.11"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "c.311T>C",
          "hgvs_p": "p.Val104Ala",
          "transcript": "ENST00000541451.5",
          "protein_id": "ENSP00000441580.1",
          "transcript_support_level": 1,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 190,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 573,
          "cdna_start": 682,
          "cdna_end": null,
          "cdna_length": 944,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000541451.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "n.739T>C",
          "hgvs_p": null,
          "transcript": "ENST00000564426.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1922,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000564426.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "n.*302T>C",
          "hgvs_p": null,
          "transcript": "ENST00000565592.5",
          "protein_id": "ENSP00000454373.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 857,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000565592.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "n.*302T>C",
          "hgvs_p": null,
          "transcript": "ENST00000565592.5",
          "protein_id": "ENSP00000454373.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 857,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000565592.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "c.509T>C",
          "hgvs_p": "p.Val170Ala",
          "transcript": "ENST00000880064.1",
          "protein_id": "ENSP00000550123.1",
          "transcript_support_level": null,
          "aa_start": 170,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 509,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": 586,
          "cdna_end": null,
          "cdna_length": 1582,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000880064.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "c.509T>C",
          "hgvs_p": "p.Val170Ala",
          "transcript": "ENST00000880062.1",
          "protein_id": "ENSP00000550121.1",
          "transcript_support_level": null,
          "aa_start": 170,
          "aa_end": null,
          "aa_length": 248,
          "cds_start": 509,
          "cds_end": null,
          "cds_length": 747,
          "cdna_start": 666,
          "cdna_end": null,
          "cdna_length": 1622,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000880062.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "c.467T>C",
          "hgvs_p": "p.Val156Ala",
          "transcript": "ENST00000927207.1",
          "protein_id": "ENSP00000597266.1",
          "transcript_support_level": null,
          "aa_start": 156,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 467,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": 540,
          "cdna_end": null,
          "cdna_length": 1496,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000927207.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "c.398T>C",
          "hgvs_p": "p.Val133Ala",
          "transcript": "ENST00000880063.1",
          "protein_id": "ENSP00000550122.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 219,
          "cds_start": 398,
          "cds_end": null,
          "cds_length": 660,
          "cdna_start": 544,
          "cdna_end": null,
          "cdna_length": 1526,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000880063.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "c.311T>C",
          "hgvs_p": "p.Val104Ala",
          "transcript": "NM_001308053.1",
          "protein_id": "NP_001294982.1",
          "transcript_support_level": null,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 190,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 573,
          "cdna_start": 682,
          "cdna_end": null,
          "cdna_length": 1688,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001308053.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "n.273T>C",
          "hgvs_p": null,
          "transcript": "ENST00000561600.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 699,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000561600.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "n.669T>C",
          "hgvs_p": null,
          "transcript": "ENST00000562735.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1720,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000562735.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "n.196T>C",
          "hgvs_p": null,
          "transcript": "ENST00000567145.2",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 609,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000567145.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SNHG19",
          "gene_hgnc_id": 49574,
          "hgvs_c": "n.217-939A>G",
          "hgvs_p": null,
          "transcript": "ENST00000720507.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 552,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000720507.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SNHG19",
          "gene_hgnc_id": 49574,
          "hgvs_c": "n.280-939A>G",
          "hgvs_p": null,
          "transcript": "ENST00000720508.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 611,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000720508.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAB26",
          "gene_hgnc_id": 14259,
          "hgvs_c": "c.-183T>C",
          "hgvs_p": null,
          "transcript": "ENST00000566724.1",
          "protein_id": "ENSP00000455675.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 135,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 644,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000566724.1"
        }
      ],
      "gene_symbol": "RAB26",
      "gene_hgnc_id": 14259,
      "dbsnp": "rs776309083",
      "frequency_reference_population": 0.0000013741757,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.00000137418,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7196350693702698,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.755,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.2307,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.02,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 2.945,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_014353.5",
          "gene_symbol": "RAB26",
          "hgnc_id": 14259,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.509T>C",
          "hgvs_p": "p.Val170Ala"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000720507.1",
          "gene_symbol": "SNHG19",
          "hgnc_id": 49574,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.217-939A>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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