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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-30721227-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=30721227&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 30721227,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000262518.9",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SRCAP",
          "gene_hgnc_id": 16974,
          "hgvs_c": "c.3292C>T",
          "hgvs_p": "p.Arg1098Trp",
          "transcript": "NM_006662.3",
          "protein_id": "NP_006653.2",
          "transcript_support_level": null,
          "aa_start": 1098,
          "aa_end": null,
          "aa_length": 3230,
          "cds_start": 3292,
          "cds_end": null,
          "cds_length": 9693,
          "cdna_start": 3647,
          "cdna_end": null,
          "cdna_length": 11724,
          "mane_select": "ENST00000262518.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SRCAP",
          "gene_hgnc_id": 16974,
          "hgvs_c": "c.3292C>T",
          "hgvs_p": "p.Arg1098Trp",
          "transcript": "ENST00000262518.9",
          "protein_id": "ENSP00000262518.4",
          "transcript_support_level": 2,
          "aa_start": 1098,
          "aa_end": null,
          "aa_length": 3230,
          "cds_start": 3292,
          "cds_end": null,
          "cds_length": 9693,
          "cdna_start": 3647,
          "cdna_end": null,
          "cdna_length": 11724,
          "mane_select": "NM_006662.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": 17,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000282034",
          "gene_hgnc_id": null,
          "hgvs_c": "n.3196+249C>T",
          "hgvs_p": null,
          "transcript": "ENST00000380361.7",
          "protein_id": "ENSP00000369719.3",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 11692,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SRCAP",
          "gene_hgnc_id": 16974,
          "hgvs_c": "c.3292C>T",
          "hgvs_p": "p.Arg1098Trp",
          "transcript": "ENST00000411466.7",
          "protein_id": "ENSP00000405186.3",
          "transcript_support_level": 3,
          "aa_start": 1098,
          "aa_end": null,
          "aa_length": 3230,
          "cds_start": 3292,
          "cds_end": null,
          "cds_length": 9693,
          "cdna_start": 3828,
          "cdna_end": null,
          "cdna_length": 11905,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SRCAP",
          "gene_hgnc_id": 16974,
          "hgvs_c": "c.3292C>T",
          "hgvs_p": "p.Arg1098Trp",
          "transcript": "ENST00000706321.1",
          "protein_id": "ENSP00000516346.1",
          "transcript_support_level": null,
          "aa_start": 1098,
          "aa_end": null,
          "aa_length": 3230,
          "cds_start": 3292,
          "cds_end": null,
          "cds_length": 9693,
          "cdna_start": 3794,
          "cdna_end": null,
          "cdna_length": 11871,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SRCAP",
          "gene_hgnc_id": 16974,
          "hgvs_c": "c.2389C>T",
          "hgvs_p": "p.Arg797Trp",
          "transcript": "ENST00000483083.3",
          "protein_id": "ENSP00000483329.1",
          "transcript_support_level": 2,
          "aa_start": 797,
          "aa_end": null,
          "aa_length": 1609,
          "cds_start": 2389,
          "cds_end": null,
          "cds_length": 4830,
          "cdna_start": 2391,
          "cdna_end": null,
          "cdna_length": 5937,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "SRCAP",
      "gene_hgnc_id": 16974,
      "dbsnp": "rs556230791",
      "frequency_reference_population": 0.000062609724,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 101,
      "gnomad_exomes_af": 0.0000670774,
      "gnomad_genomes_af": 0.0000197148,
      "gnomad_exomes_ac": 98,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.19920867681503296,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.37,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1861,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.05,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 0.351,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -7,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Moderate,BP6,BS2",
      "acmg_by_gene": [
        {
          "score": -7,
          "benign_score": 7,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000262518.9",
          "gene_symbol": "SRCAP",
          "hgnc_id": 16974,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.3292C>T",
          "hgvs_p": "p.Arg1098Trp"
        },
        {
          "score": -1,
          "benign_score": 3,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000380361.7",
          "gene_symbol": "ENSG00000282034",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.3196+249C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " and behavioral abnormalities, hypotonia, musculoskeletal defects,Developmental delay,Floating-Harbor syndrome,not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:1 LB:1 B:1",
      "phenotype_combined": "not specified|Floating-Harbor syndrome;Developmental delay, hypotonia, musculoskeletal defects, and behavioral abnormalities|not provided",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}